FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034309853> ?p ?o ?g. }

• W2034309853 endingPage "1488" @default.
• W2034309853 startingPage "1474" @default.
• W2034309853 abstract "Gene delivery from a substrate depends, in part, on the vector-nucleic acid complex that is bound to the surface and the cell adhesive properties of the surface. Here, we present a method to deliver patterns of small interfering RNA (siRNA) that capitalize on a forward transfection method (transfection by introducing siRNA transfection reagent complexes onto plated cells); herein denoted as multilayer mediated forward transfection (MFT). This method separates the substrate-mediated delivery from the cell adhesive properties of the surface. pH responsive layer-by-layer (LbL) assembled multilayers were used as the delivery platform and microcontact printing technique (microCP) was used to pattern nanoparticles of transfection reagent-siRNA complexes onto degradable multilayers. Efficient MFT depend on optimal formulation of the nanoparticles. 25 kDa linear polyethylenimine (LPEI) was optimized as the siRNA transfection reagent for normal forward transfection (NFT) of the nanoparticles. A broad range of LPEI-siRNA nitrogen/phosphate (N/P) ratios (ranging from 5 to 90) was evaluated for the relative amounts of siRNA incorporated into the nanoparticles, nanoparticle size and NFT efficiencies. All the siRNA was incorporated into the nanoparticles at N/P ratio near 90. Increasing the amount of siRNA incorporated into the nanoparticles, with increasing N/P ratio correlated with a linear blue shift in the ultraviolet/visible (UV/vis) absorbance spectrum of the LPEI-siRNA nanoparticles. NFT efficiency greater than 80% was achieved with minimal cytotoxicity at N/P ratio of 30 and siRNA concentration of 200 nM. Similarly, MFT efficiency 60% was achieved for LPEI-siRNA nanoparticles at N/P ratios greater than 30." @default.
• W2034309853 created "2016-06-24" @default.
• W2034309853 creator A5008332310 @default.
• W2034309853 creator A5035466229 @default.
• W2034309853 creator A5041822368 @default.
• W2034309853 date "2009-06-01" @default.
• W2034309853 modified "2023-10-14" @default.
• W2034309853 title "Multilayer mediated forward and patterned siRNA transfection using linear-PEI at extended N/P ratios" @default.
• W2034309853 cites W1536781994 @default.
• W2034309853 cites W1611419331 @default.
• W2034309853 cites W1647075334 @default.
• W2034309853 cites W1669562987 @default.
• W2034309853 cites W1969233927 @default.
• W2034309853 cites W1970594627 @default.
• W2034309853 cites W1973458116 @default.
• W2034309853 cites W1976600729 @default.
• W2034309853 cites W1979181108 @default.
• W2034309853 cites W1979519758 @default.
• W2034309853 cites W1984568212 @default.
• W2034309853 cites W1984739176 @default.
• W2034309853 cites W1985309114 @default.
• W2034309853 cites W1994070458 @default.
• W2034309853 cites W1997523705 @default.
• W2034309853 cites W1997993853 @default.
• W2034309853 cites W1997998868 @default.
• W2034309853 cites W2004786617 @default.
• W2034309853 cites W2008317773 @default.
• W2034309853 cites W2009842679 @default.
• W2034309853 cites W2010655741 @default.
• W2034309853 cites W2032036225 @default.
• W2034309853 cites W2032576390 @default.
• W2034309853 cites W2043996751 @default.
• W2034309853 cites W2048928172 @default.
• W2034309853 cites W2049404770 @default.
• W2034309853 cites W2052431279 @default.
• W2034309853 cites W2055620528 @default.
• W2034309853 cites W2056145482 @default.
• W2034309853 cites W2056579820 @default.
• W2034309853 cites W2056618936 @default.
• W2034309853 cites W2063699230 @default.
• W2034309853 cites W2069442173 @default.
• W2034309853 cites W2074870430 @default.
• W2034309853 cites W2077112895 @default.
• W2034309853 cites W2077139193 @default.
• W2034309853 cites W2077520283 @default.
• W2034309853 cites W2078377919 @default.
• W2034309853 cites W2091211551 @default.
• W2034309853 cites W2092049057 @default.
• W2034309853 cites W2099113192 @default.
• W2034309853 cites W2101784587 @default.
• W2034309853 cites W2109749144 @default.
• W2034309853 cites W2110385304 @default.
• W2034309853 cites W2116150864 @default.
• W2034309853 cites W2117208350 @default.
• W2034309853 cites W2121617922 @default.
• W2034309853 cites W2124229990 @default.
• W2034309853 cites W2150941172 @default.
• W2034309853 cites W2158395637 @default.
• W2034309853 cites W2163921171 @default.
• W2034309853 cites W4248033625 @default.
• W2034309853 doi "https://doi.org/10.1016/j.actbio.2009.01.004" @default.
• W2034309853 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2677632" @default.
• W2034309853 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19217360" @default.
• W2034309853 hasPublicationYear "2009" @default.
• W2034309853 type Work @default.
• W2034309853 sameAs 2034309853 @default.
• W2034309853 citedByCount "55" @default.
• W2034309853 countsByYear W20343098532012 @default.
• W2034309853 countsByYear W20343098532013 @default.
• W2034309853 countsByYear W20343098532014 @default.
• W2034309853 countsByYear W20343098532015 @default.
• W2034309853 countsByYear W20343098532016 @default.
• W2034309853 countsByYear W20343098532017 @default.
• W2034309853 countsByYear W20343098532018 @default.
• W2034309853 countsByYear W20343098532019 @default.
• W2034309853 countsByYear W20343098532020 @default.
• W2034309853 countsByYear W20343098532021 @default.
• W2034309853 countsByYear W20343098532022 @default.
• W2034309853 crossrefType "journal-article" @default.
• W2034309853 hasAuthorship W2034309853A5008332310 @default.
• W2034309853 hasAuthorship W2034309853A5035466229 @default.
• W2034309853 hasAuthorship W2034309853A5041822368 @default.
• W2034309853 hasBestOaLocation W20343098532 @default.
• W2034309853 hasConcept C104317684 @default.
• W2034309853 hasConcept C109316439 @default.
• W2034309853 hasConcept C12554922 @default.
• W2034309853 hasConcept C155672457 @default.
• W2034309853 hasConcept C171250308 @default.
• W2034309853 hasConcept C185592680 @default.
• W2034309853 hasConcept C192562407 @default.
• W2034309853 hasConcept C202751555 @default.
• W2034309853 hasConcept C22615655 @default.
• W2034309853 hasConcept C24107716 @default.
• W2034309853 hasConcept C2775925850 @default.
• W2034309853 hasConcept C43617362 @default.
• W2034309853 hasConcept C54009773 @default.
• W2034309853 hasConcept C55493867 @default.
• W2034309853 hasConcept C86803240 @default.

• next