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• W2034315297 abstract "In this study, the 23S rRNA genes of nine different Pediococcus type strains were sequenced. By using a multiple sequence alignment with 23S rDNA sequences of related lactic acid bacteria two primer pairs were constructed, one for the general identification of the genus Pediococcus and one for the identification of the atypical species, P. dextrinicus. Furthermore, a primer set for a rapid multiplex PCR identification of the eight typical Pediococcus species was developed. With this technique, the species P. damnosus, P. parvulus, P. inopinatus, P. cellicola, P. pentosaceus, P. acidilactici, P. claussenii, and P. stilesii could be discriminated simultaneously in a single PCR. Experiments with inoculated grape musts showed that the detection limit was 10 cells ml− 1. The multiplex PCR assay was tested by the usage of 62 Pediococcus strains from different culture collections and 47 strains recently isolated from German wines and musts. In addition, contaminations with P. parvulus and P. damnosus could be detected after purification of DNA from spoilt wine samples. The method demonstrates a rapid and easy to handle tool for the species affiliation of pediococci in beverages and food samples." @default.
• W2034315297 created "2016-06-24" @default.
• W2034315297 creator A5039491799 @default.
• W2034315297 creator A5087353880 @default.
• W2034315297 date "2008-12-01" @default.
• W2034315297 modified "2023-10-14" @default.
• W2034315297 title "Use of a species-specific multiplex PCR for the identification of pediococci" @default.
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• W2034315297 doi "https://doi.org/10.1016/j.ijfoodmicro.2008.08.019" @default.
• W2034315297 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18835501" @default.
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