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- W2034330286 abstract "We investigated the effect of a finite-sized confocal pinhole on the performance of nonlinear optical microscopes based on two-photon excited fluorescence and second-harmonic generation. These techniques were implemented using a modified inverted commercial confocal microscope coupled to a femtosecond Ti:sapphire laser. Both the transverse and axial resolutions are improved when the confocal pinhole is used, albeit at the expense of the signal level. Therefore, the routine use of a confocal pinhole of optimized size is recommended for two-photon microscopy wherever the fluorescence or harmonic signals are large." @default.
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- W2034330286 date "1999-11-01" @default.
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- W2034330286 title "Effect of a confocal pinhole in two-photon microscopy" @default.
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- W2034330286 doi "https://doi.org/10.1002/(sici)1097-0029(19991101)47:3<210::aid-jemt7>3.0.co;2-h" @default.
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