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- W2034331960 abstract "Maxicircle DNA has been isolated from the kinetoplast DNA of Leishmania tarentolae culture forms by bouyant separation in CsCl in the presence of the A + T-binding dye Hoechst 33258, after liberation from the kinetoplast DNA network by cleavage with the single-hit restriction endonuclease EcoRI. The purified linearized maxicircle DNA has a density in CsCl of 1.681 g/cm3 (79% A + T) and a molecular weight of approximately 18-20 x 10(6). The maxicircle molecule exhibited intramolecular base composition heterogeneity ranging from 85% A + T to 74% A + T." @default.
- W2034331960 created "2016-06-24" @default.
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- W2034331960 date "1979-04-01" @default.
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- W2034331960 title "Isolation of maxicircle component of kinetoplast DNA from hemoflagellate protozoa." @default.
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- W2034331960 doi "https://doi.org/10.1073/pnas.76.4.1585" @default.
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