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• W2034338541 abstract "BACKGROUND: The clinical application of flow cytometric direct antiglobulin test (FC‐DAT) has rarely been evaluated for patients with various diseases including immune and nonimmune hemolytic anemia. STUDY DESIGN AND METHODS: Blood samples from 380 patients with a variety of diseases were studied using the tube direct DAT and FC‐DAT. The results of tube DAT and FC‐DAT were compared. The predictive values of DAT for hemolysis were evaluated. RESULTS: Of 57 patients with autoimmune hemolytic anemia (AIHA), 6 of the 17 with a negative tube DAT (immunoglobulin G [IgG]) had a positive FC‐DAT (IgG) and 23 of the 36 patients with a negative tube DAT (complement 3d [C3d]) had a positive FC‐DAT (C3d). In 57 patients with AIHA, the incidence of positive results of FC‐DAT (IgG) and tube DAT (IgG) were similar (42 positive vs. 40 positive); but in 323 patients without AIHA, the incidence of positive FC‐DATs (IgG) was higher than that of tube DAT (IgG; 47 positive vs. 9 positive). The higher incidence of positive FC‐DAT (C3d) than that of tube DAT (C3d) was seen in patients with AIHA (42 positive vs. 21 positive) as well as in patients without AIHA (61 positive vs. 5 positive). Both DAT (IgG) and DAT (C3d) positive has highest positive predictive value for hemolysis, followed by DAT (IgG) alone positive and DAT (C3d) alone positive. CONCLUSIONS: FC‐DAT is a complementary test for diagnosing AIHA. There is a synergistic effect of the red blood cell–bound IgG and complement in predicting hemolysis." @default.
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• W2034338541 date "2009-07-01" @default.
• W2034338541 modified "2023-10-16" @default.
• W2034338541 title "Clinical application of a flow cytometric direct antiglobulin test" @default.
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• W2034338541 doi "https://doi.org/10.1111/j.1537-2995.2009.02130.x" @default.
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