FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034339355> ?p ?o ?g. }

• W2034339355 endingPage "938" @default.
• W2034339355 startingPage "930" @default.
• W2034339355 abstract "Air pollution with fine particulates (PM(10) and PM(2.5)) is associated with an increased incidence of cardiovascular events. The proposed mechanisms include indirect proinflammatory and procoagulant reactions involving activation of pulmonary macrophages, endothelial cells and the TNF/TF pathway, or direct procoagulant effects. Our laboratory has observed a reduction of the platelet responsiveness to collagen after exposure to diesel exhaust particles (DEP).DEP directly interfere with platelet-collagen interactions by selectively inducing the shedding of platelet signaling receptors via metalloproteinases, which would represent a novel mechanism for DEP action on platelets.Citrated blood from healthy volunteers was exposed to highly standardized DEP at concentrations of 0.1, 2.5 and 5.0μg/ml or ultrafine carbon black (ufCB, 0.1μg/ml) and the plasmatic and platelet response was analysed. The closure times with the PFA-100 device and the platelet aggregation in response to a variety of agonists were monitored. Interleukins (IL)-1β and IL-8 levels were determined by ELISA and soluble P-selectin by the Luminex bead assay. Thrombin activity was measured as the endogenous thrombin potential (ETP) by fluorescence spectrometry. Soluble GPVI and GPIbα (glycocalicin) ectodomain fragments were measured by ELISA. ADAMTS13 activity was determined by a FRETS based assay and plasmin activity with Spectrozyme PL.Aggregation assays where platelets were treated with low dose DEP or ultrafine carbon black (ufCB) revealed a significantly increased response to low doses of collagen (p<0.05, n=5). At higher doses, however, collagen induced aggregation was suppressed by DEP treatment: at 2.5μg/ml, the inhibition was 34±12% (p<0.01, n=10). Aggregations with cross-linked collagen related peptide (CRPxl), convulxin and with the monoclonal antibody 9O12.2 (all known to specifically bind to and activate GPVI) were also diminished. Ristocetin, arachidonic acid and ADP responses were normal at all DEP concentrations. No cleavage of GPVI ectodomain was detected (soluble GPVI 27.8±3 vs. 28±4μg/ml mean±SEM, n=10); however increased plasma glycocalicin (GPIbα ectodomain) was detected upon diesel exposure (2.58±0.11 vs. 2.28±0.03μg/ml p<0.01, n=10). ADAMTS13 and plasmin activity remained unaffected by DEP under the conditions tested. Platelets were not activated by either DEP or ufCB as soluble P-selectin was insensitive to these.DEP specifically and directly interferes with platelet-collagen interactions. The functional consequences are biphasic and include enhance platelet aggregation at lower DEP concentrations and inhibition at a higher dose. Our data indicate that this interaction does not involve P-selectin or GPVI shedding. It is however associated with an increase in GPIb cleavage." @default.
• W2034339355 created "2016-06-24" @default.
• W2034339355 creator A5000218370 @default.
• W2034339355 creator A5007424744 @default.
• W2034339355 creator A5019077055 @default.
• W2034339355 creator A5072400072 @default.
• W2034339355 creator A5074030410 @default.
• W2034339355 creator A5082733512 @default.
• W2034339355 date "2012-09-01" @default.
• W2034339355 modified "2023-09-25" @default.
• W2034339355 title "Diesel exhaust particles impair platelet response to collagen and are associated with GPIbα shedding" @default.
• W2034339355 cites W112829769 @default.
• W2034339355 cites W1535628758 @default.
• W2034339355 cites W1578041684 @default.
• W2034339355 cites W1585648374 @default.
• W2034339355 cites W1894101376 @default.
• W2034339355 cites W1968652207 @default.
• W2034339355 cites W1968715623 @default.
• W2034339355 cites W1973804775 @default.
• W2034339355 cites W1991880580 @default.
• W2034339355 cites W1994860164 @default.
• W2034339355 cites W1997269140 @default.
• W2034339355 cites W1998726594 @default.
• W2034339355 cites W2012974033 @default.
• W2034339355 cites W2022014262 @default.
• W2034339355 cites W2029575669 @default.
• W2034339355 cites W2030337128 @default.
• W2034339355 cites W2032026482 @default.
• W2034339355 cites W2033536448 @default.
• W2034339355 cites W2035510249 @default.
• W2034339355 cites W2039998479 @default.
• W2034339355 cites W2054806718 @default.
• W2034339355 cites W2058788873 @default.
• W2034339355 cites W2061400583 @default.
• W2034339355 cites W2066878158 @default.
• W2034339355 cites W2070976367 @default.
• W2034339355 cites W2087560920 @default.
• W2034339355 cites W2088518336 @default.
• W2034339355 cites W2104761649 @default.
• W2034339355 cites W2106344630 @default.
• W2034339355 cites W2107115075 @default.
• W2034339355 cites W2109421983 @default.
• W2034339355 cites W2112314423 @default.
• W2034339355 cites W2117196934 @default.
• W2034339355 cites W2120823353 @default.
• W2034339355 cites W2124700528 @default.
• W2034339355 cites W2135486988 @default.
• W2034339355 cites W2138211885 @default.
• W2034339355 cites W2140639765 @default.
• W2034339355 cites W2141905650 @default.
• W2034339355 cites W2144344073 @default.
• W2034339355 cites W2145657011 @default.
• W2034339355 cites W2146532249 @default.
• W2034339355 cites W2146548391 @default.
• W2034339355 cites W2147152693 @default.
• W2034339355 cites W2155133994 @default.
• W2034339355 cites W2156573675 @default.
• W2034339355 cites W2167905528 @default.
• W2034339355 cites W2170614009 @default.
• W2034339355 cites W2316074233 @default.
• W2034339355 cites W2558772394 @default.
• W2034339355 cites W27185196 @default.
• W2034339355 cites W4250054871 @default.
• W2034339355 doi "https://doi.org/10.1016/j.tiv.2012.04.009" @default.
• W2034339355 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22542759" @default.
• W2034339355 hasPublicationYear "2012" @default.
• W2034339355 type Work @default.
• W2034339355 sameAs 2034339355 @default.
• W2034339355 citedByCount "13" @default.
• W2034339355 countsByYear W20343393552013 @default.
• W2034339355 countsByYear W20343393552014 @default.
• W2034339355 countsByYear W20343393552015 @default.
• W2034339355 countsByYear W20343393552016 @default.
• W2034339355 countsByYear W20343393552017 @default.
• W2034339355 countsByYear W20343393552018 @default.
• W2034339355 countsByYear W20343393552020 @default.
• W2034339355 countsByYear W20343393552021 @default.
• W2034339355 crossrefType "journal-article" @default.
• W2034339355 hasAuthorship W2034339355A5000218370 @default.
• W2034339355 hasAuthorship W2034339355A5007424744 @default.
• W2034339355 hasAuthorship W2034339355A5019077055 @default.
• W2034339355 hasAuthorship W2034339355A5072400072 @default.
• W2034339355 hasAuthorship W2034339355A5074030410 @default.
• W2034339355 hasAuthorship W2034339355A5082733512 @default.
• W2034339355 hasConcept C12554922 @default.
• W2034339355 hasConcept C164027704 @default.
• W2034339355 hasConcept C185592680 @default.
• W2034339355 hasConcept C203014093 @default.
• W2034339355 hasConcept C2776914184 @default.
• W2034339355 hasConcept C2777292125 @default.
• W2034339355 hasConcept C2780573682 @default.
• W2034339355 hasConcept C3018697912 @default.
• W2034339355 hasConcept C71924100 @default.
• W2034339355 hasConcept C86803240 @default.
• W2034339355 hasConcept C89560881 @default.
• W2034339355 hasConcept C98274493 @default.
• W2034339355 hasConceptScore W2034339355C12554922 @default.
• W2034339355 hasConceptScore W2034339355C164027704 @default.

• next