FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034342274> ?p ?o ?g. }

• W2034342274 endingPage "44" @default.
• W2034342274 startingPage "33" @default.
• W2034342274 abstract "As the increasing demand from both chemical and fuel markets, the interest in producing n-butanol using biological route has been rejuvenated to engineer an economical fermentation process, competing with the currently-dominant chemical synthesis. n-Butanol has been traditionally produced from the ABE fermentation of Clostridium acetobutylicum. This system, however, is not economically feasible due to its limited efficiency and the lack of genetic modification tools for further improvements. Alternatively, n-butanol synthesis pathway was successfully transferred into Escherichia coli and rapidly improved to reach a level of production comparable to the native producer. Nevertheless, the toxicity of n-butanol has become a common issue that either approach has to deal with. Previously, we reported our success in improving n-butanol tolerance in E. coli by engineering an Artificial Transcription Factor (ATF) that can modify the expression level of multiple targets simultaneously and improved the n-butanol tolerance of MG1655 strain to 1.5% (vol/vol) n-butanol. However, it was observed that some possible n-butanol tolerance mechanisms did not occurred upon the ATF expression, especially the membrane-related functions such as the homeoviscous adaptation, iron uptaking system, and efflux pump system. In this work, we attempted to enhance the n-butanol tolerance associated with the ATF by combining it with the membrane-related functions in E. coli, including the overexpression of fatty acid synthesis genes, iron-uptaking protein FeoA, and introducing a SrpABC efflux pump from Pseudomonas putida into E. coli. The synergistic effect of this combinatorial approach led to 4, 5, and 9-fold improved growths in the cultures containing 1, 1.5, and 2% (vol/vol) n-butanol, respectively, of an MG1655 knockout strain engineered for n-butanol production, and expanded the tolerance limit to 2% (vol/vol) n-butanol." @default.
• W2034342274 created "2016-06-24" @default.
• W2034342274 creator A5017735053 @default.
• W2034342274 creator A5024505376 @default.
• W2034342274 creator A5049367336 @default.
• W2034342274 creator A5054497310 @default.
• W2034342274 creator A5082154194 @default.
• W2034342274 creator A5086006406 @default.
• W2034342274 date "2015-06-01" @default.
• W2034342274 modified "2023-10-02" @default.
• W2034342274 title "Improved n-butanol tolerance in Escherichia coli by controlling membrane related functions" @default.
• W2034342274 cites W1560135653 @default.
• W2034342274 cites W1560754586 @default.
• W2034342274 cites W1621330152 @default.
• W2034342274 cites W1957671946 @default.
• W2034342274 cites W1969975379 @default.
• W2034342274 cites W1972549326 @default.
• W2034342274 cites W1975276775 @default.
• W2034342274 cites W1976685082 @default.
• W2034342274 cites W1977513597 @default.
• W2034342274 cites W1979041750 @default.
• W2034342274 cites W1981717477 @default.
• W2034342274 cites W1999912067 @default.
• W2034342274 cites W2000427711 @default.
• W2034342274 cites W2020366340 @default.
• W2034342274 cites W2025311938 @default.
• W2034342274 cites W2033641514 @default.
• W2034342274 cites W2042049515 @default.
• W2034342274 cites W2043105926 @default.
• W2034342274 cites W2052461285 @default.
• W2034342274 cites W2053112566 @default.
• W2034342274 cites W2057798220 @default.
• W2034342274 cites W2067015179 @default.
• W2034342274 cites W2069425295 @default.
• W2034342274 cites W2070851723 @default.
• W2034342274 cites W2079802325 @default.
• W2034342274 cites W2087561640 @default.
• W2034342274 cites W2088363265 @default.
• W2034342274 cites W2090107871 @default.
• W2034342274 cites W2091342962 @default.
• W2034342274 cites W2099065852 @default.
• W2034342274 cites W2106000224 @default.
• W2034342274 cites W2111967267 @default.
• W2034342274 cites W2116513894 @default.
• W2034342274 cites W2124078475 @default.
• W2034342274 cites W2128363716 @default.
• W2034342274 cites W2129026291 @default.
• W2034342274 cites W2129858956 @default.
• W2034342274 cites W2132202488 @default.
• W2034342274 cites W2132892146 @default.
• W2034342274 cites W2134153749 @default.
• W2034342274 cites W2138231210 @default.
• W2034342274 cites W2142251632 @default.
• W2034342274 cites W2148590972 @default.
• W2034342274 cites W2155866836 @default.
• W2034342274 cites W2158571208 @default.
• W2034342274 cites W2160944601 @default.
• W2034342274 cites W2170466099 @default.
• W2034342274 cites W24509859 @default.
• W2034342274 doi "https://doi.org/10.1016/j.jbiotec.2015.03.025" @default.
• W2034342274 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25858152" @default.
• W2034342274 hasPublicationYear "2015" @default.
• W2034342274 type Work @default.
• W2034342274 sameAs 2034342274 @default.
• W2034342274 citedByCount "44" @default.
• W2034342274 countsByYear W20343422742015 @default.
• W2034342274 countsByYear W20343422742016 @default.
• W2034342274 countsByYear W20343422742017 @default.
• W2034342274 countsByYear W20343422742018 @default.
• W2034342274 countsByYear W20343422742019 @default.
• W2034342274 countsByYear W20343422742020 @default.
• W2034342274 countsByYear W20343422742021 @default.
• W2034342274 countsByYear W20343422742022 @default.
• W2034342274 countsByYear W20343422742023 @default.
• W2034342274 crossrefType "journal-article" @default.
• W2034342274 hasAuthorship W2034342274A5017735053 @default.
• W2034342274 hasAuthorship W2034342274A5024505376 @default.
• W2034342274 hasAuthorship W2034342274A5049367336 @default.
• W2034342274 hasAuthorship W2034342274A5054497310 @default.
• W2034342274 hasAuthorship W2034342274A5082154194 @default.
• W2034342274 hasAuthorship W2034342274A5086006406 @default.
• W2034342274 hasBestOaLocation W20343422741 @default.
• W2034342274 hasConcept C100544194 @default.
• W2034342274 hasConcept C104317684 @default.
• W2034342274 hasConcept C185592680 @default.
• W2034342274 hasConcept C200082930 @default.
• W2034342274 hasConcept C2776928978 @default.
• W2034342274 hasConcept C2777272437 @default.
• W2034342274 hasConcept C2777713963 @default.
• W2034342274 hasConcept C2780161600 @default.
• W2034342274 hasConcept C2780543182 @default.
• W2034342274 hasConcept C523546767 @default.
• W2034342274 hasConcept C54355233 @default.
• W2034342274 hasConcept C547475151 @default.
• W2034342274 hasConcept C55493867 @default.
• W2034342274 hasConcept C6350086 @default.
• W2034342274 hasConcept C86803240 @default.
• W2034342274 hasConceptScore W2034342274C100544194 @default.

• next