FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034343384> ?p ?o ?g. }

• W2034343384 endingPage "145" @default.
• W2034343384 startingPage "135" @default.
• W2034343384 abstract "Summary The oocyte is known from recent studies in the mouse, cow, sheep and human to be a central regulator of follicular cell function. However, in the pig, little information is known about the regulation of cumulus expansion by oocyte-secreted factors and oocyte quality. We investigated the possible effects of oocyte-secreted factors during in vitro maturation on cumulus expansion and on porcine oocytes as judged by subsequent embryonic development after parthenogenetic activation. Cumulus–oocyte complexes (COC) from antral follicles of pig ovaries collected from a local abattoir were divided into control and treatment groups and were cultured in tissue culture medium 199 supplemented with follicle-stimulating hormone. Treatment groups consisted of increasing numbers of denuded oocytes (DO) co-cultured with COC (at ratios of COC to DO of 1:1, 1:2, 1:3, 1:4 and 1:5). After incubation for 44 h, cumulus expansion and maturation rates were assessed and oocytes were activated parthenogenetically. Cumulus expansion in the 1 COC:4 DO and 1 COC:5 DO groups was low and altered because full dispersion of the outer layer did not occur. Cell viability was not affected, as measured by the automated cell counter, but scanning electron microscopy revealed only a scanty extracellular matrix. Blastocyst rate was significantly higher in the 1 COC:4 DO (34.4%) and in the 1 COC:5 DO (34.9%) groups ( p < 0.05) when compared with other groups. Maturation rate, cleavage rate and total cell number showed no significant difference between control and treatment groups. Amplification by reverse transcription polymerase chain reaction (RT-PCR) showed up-regulation of growth differentiation factor 9 (GDF9) in the cumulus cells in the 1 COC:4 DO group at 44 h. We conclude that denuded porcine oocytes could improve the maturation of COC as evidenced by increased blastocyst development in the 1 COC:4 DO, even though cumulus expansion was poor. This improvement could be a result of the GDF9 up-regulation." @default.
• W2034343384 created "2016-06-24" @default.
• W2034343384 creator A5018791841 @default.
• W2034343384 creator A5025152012 @default.
• W2034343384 creator A5032322155 @default.
• W2034343384 creator A5041650772 @default.
• W2034343384 creator A5042921298 @default.
• W2034343384 creator A5052398257 @default.
• W2034343384 creator A5054305609 @default.
• W2034343384 creator A5063668393 @default.
• W2034343384 creator A5065490021 @default.
• W2034343384 creator A5072124738 @default.
• W2034343384 date "2011-07-27" @default.
• W2034343384 modified "2023-09-27" @default.
• W2034343384 title "Effect of oocyte-secreted factors on porcine<i>in vitro</i>maturation, cumulus expansion and developmental competence of parthenotes" @default.
• W2034343384 cites W1501791679 @default.
• W2034343384 cites W1551944091 @default.
• W2034343384 cites W1968945784 @default.
• W2034343384 cites W1972324826 @default.
• W2034343384 cites W1973628165 @default.
• W2034343384 cites W1978258909 @default.
• W2034343384 cites W1980102452 @default.
• W2034343384 cites W1987610083 @default.
• W2034343384 cites W1991109392 @default.
• W2034343384 cites W1997492405 @default.
• W2034343384 cites W1999470156 @default.
• W2034343384 cites W2008927386 @default.
• W2034343384 cites W2016570571 @default.
• W2034343384 cites W2018615497 @default.
• W2034343384 cites W2018831893 @default.
• W2034343384 cites W2020765273 @default.
• W2034343384 cites W2035048391 @default.
• W2034343384 cites W2041944050 @default.
• W2034343384 cites W2042292319 @default.
• W2034343384 cites W2044933887 @default.
• W2034343384 cites W2056248345 @default.
• W2034343384 cites W2057336811 @default.
• W2034343384 cites W2058732512 @default.
• W2034343384 cites W2061026525 @default.
• W2034343384 cites W2072439295 @default.
• W2034343384 cites W2076565229 @default.
• W2034343384 cites W2077243848 @default.
• W2034343384 cites W2088235456 @default.
• W2034343384 cites W2091319584 @default.
• W2034343384 cites W2097665482 @default.
• W2034343384 cites W2098519379 @default.
• W2034343384 cites W2100729936 @default.
• W2034343384 cites W2106487699 @default.
• W2034343384 cites W2110553915 @default.
• W2034343384 cites W2126722856 @default.
• W2034343384 cites W2129012279 @default.
• W2034343384 cites W2129585892 @default.
• W2034343384 cites W2129868058 @default.
• W2034343384 cites W2130897208 @default.
• W2034343384 cites W2131311271 @default.
• W2034343384 cites W2135104064 @default.
• W2034343384 cites W2135110241 @default.
• W2034343384 cites W2139624389 @default.
• W2034343384 cites W2149467232 @default.
• W2034343384 cites W2153323265 @default.
• W2034343384 cites W2155970941 @default.
• W2034343384 cites W2161054992 @default.
• W2034343384 cites W2161219665 @default.
• W2034343384 cites W2168542793 @default.
• W2034343384 cites W2170707255 @default.
• W2034343384 cites W2237969214 @default.
• W2034343384 doi "https://doi.org/10.1017/s0967199411000256" @default.
• W2034343384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21791167" @default.
• W2034343384 hasPublicationYear "2011" @default.
• W2034343384 type Work @default.
• W2034343384 sameAs 2034343384 @default.
• W2034343384 citedByCount "27" @default.
• W2034343384 countsByYear W20343433842012 @default.
• W2034343384 countsByYear W20343433842013 @default.
• W2034343384 countsByYear W20343433842014 @default.
• W2034343384 countsByYear W20343433842015 @default.
• W2034343384 countsByYear W20343433842016 @default.
• W2034343384 countsByYear W20343433842017 @default.
• W2034343384 countsByYear W20343433842018 @default.
• W2034343384 countsByYear W20343433842023 @default.
• W2034343384 crossrefType "journal-article" @default.
• W2034343384 hasAuthorship W2034343384A5018791841 @default.
• W2034343384 hasAuthorship W2034343384A5025152012 @default.
• W2034343384 hasAuthorship W2034343384A5032322155 @default.
• W2034343384 hasAuthorship W2034343384A5041650772 @default.
• W2034343384 hasAuthorship W2034343384A5042921298 @default.
• W2034343384 hasAuthorship W2034343384A5052398257 @default.
• W2034343384 hasAuthorship W2034343384A5054305609 @default.
• W2034343384 hasAuthorship W2034343384A5063668393 @default.
• W2034343384 hasAuthorship W2034343384A5065490021 @default.
• W2034343384 hasAuthorship W2034343384A5072124738 @default.
• W2034343384 hasConcept C134018914 @default.
• W2034343384 hasConcept C16685009 @default.
• W2034343384 hasConcept C185592680 @default.
• W2034343384 hasConcept C187785154 @default.
• W2034343384 hasConcept C196843134 @default.
• W2034343384 hasConcept C2775966511 @default.
• W2034343384 hasConcept C2776690073 @default.

• next