FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034347824> ?p ?o ?g. }

• W2034347824 abstract "Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful method for the analysis of gene expression. Target gene expression levels are usually normalized to a consistently expressed reference gene also known as internal standard, in the same sample. However, much effort has not been expended thus far in the search for reference genes suitable for the study of stomach cancer using RT-qPCR, although selection of optimal reference genes is critical for interpretation of results.We assessed the suitability of six possible reference genes, beta-actin (ACTB), glyceraldehydes-3-phosphate dehydrogenase (GAPDH), hypoxanthine phosphoribosyl transferase 1 (HPRT1), beta-2-microglobulin (B2M), ribosomal subunit L29 (RPL29) and 18S ribosomal RNA (18S rRNA) in 20 normal and tumor stomach tissue pairs of stomach cancer patients and 6 stomach cancer cell lines, by RT-qPCR. Employing expression stability analyses using NormFinder and geNorm algorithms we determined the order of performance of these reference genes and their variation values.This RT-qPCR study showed that there are statistically significant (p < 0.05) differences in the expression levels of HPRT1 and 18S rRNA in 'normal-' versus 'tumor stomach tissues'. The stability analyses by geNorm suggest B2M-GAPDH, as best reference gene combination for 'stomach cancer cell lines'; RPL29-HPRT1, for 'all stomach tissues'; and ACTB-18S rRNA, for 'all stomach cell lines and tissues'. NormFinder also identified B2M as the best reference gene for 'stomach cancer cell lines', RPL29-B2M for 'all stomach tissues', and 18S rRNA-ACTB for 'all stomach cell lines and tissues'. The comparisons of normalized expression of the target gene, GPNMB, showed different interpretation of target gene expression depend on best single reference gene or combination.This study validated RPL29 and RPL29-B2M as the best single reference genes and combination, for RT-qPCR analysis of 'all stomach tissues', and B2M and B2M-GAPDH as the best single reference gene and combination, for 'stomach cancer cell lines'. Use of these validated reference genes should provide more exact interpretation of differential gene expressions at transcription level in stomach cancer." @default.
• W2034347824 created "2016-06-24" @default.
• W2034347824 creator A5000169747 @default.
• W2034347824 creator A5004383934 @default.
• W2034347824 creator A5004729412 @default.
• W2034347824 creator A5028915237 @default.
• W2034347824 creator A5049462378 @default.
• W2034347824 creator A5072924257 @default.
• W2034347824 date "2010-05-28" @default.
• W2034347824 modified "2023-10-10" @default.
• W2034347824 title "Identification of valid reference genes for gene expression studies of human stomach cancer by reverse transcription-qPCR" @default.
• W2034347824 cites W1577577364 @default.
• W2034347824 cites W1923010649 @default.
• W2034347824 cites W1964507233 @default.
• W2034347824 cites W1973666259 @default.
• W2034347824 cites W1983241347 @default.
• W2034347824 cites W1986080495 @default.
• W2034347824 cites W1991760543 @default.
• W2034347824 cites W2000944914 @default.
• W2034347824 cites W2009467250 @default.
• W2034347824 cites W2020830322 @default.
• W2034347824 cites W2027870335 @default.
• W2034347824 cites W2036060339 @default.
• W2034347824 cites W2049619530 @default.
• W2034347824 cites W2065270021 @default.
• W2034347824 cites W2068663152 @default.
• W2034347824 cites W2088494800 @default.
• W2034347824 cites W2099824652 @default.
• W2034347824 cites W2105423509 @default.
• W2034347824 cites W2120557839 @default.
• W2034347824 cites W2130805194 @default.
• W2034347824 cites W2133585947 @default.
• W2034347824 cites W2134373876 @default.
• W2034347824 cites W2145611583 @default.
• W2034347824 cites W2145879504 @default.
• W2034347824 cites W2154273881 @default.
• W2034347824 cites W2156664292 @default.
• W2034347824 cites W2158525886 @default.
• W2034347824 cites W2163304390 @default.
• W2034347824 cites W2168420558 @default.
• W2034347824 doi "https://doi.org/10.1186/1471-2407-10-240" @default.
• W2034347824 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2887403" @default.
• W2034347824 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20507635" @default.
• W2034347824 hasPublicationYear "2010" @default.
• W2034347824 type Work @default.
• W2034347824 sameAs 2034347824 @default.
• W2034347824 citedByCount "81" @default.
• W2034347824 countsByYear W20343478242012 @default.
• W2034347824 countsByYear W20343478242013 @default.
• W2034347824 countsByYear W20343478242014 @default.
• W2034347824 countsByYear W20343478242015 @default.
• W2034347824 countsByYear W20343478242016 @default.
• W2034347824 countsByYear W20343478242017 @default.
• W2034347824 countsByYear W20343478242018 @default.
• W2034347824 countsByYear W20343478242019 @default.
• W2034347824 countsByYear W20343478242020 @default.
• W2034347824 countsByYear W20343478242021 @default.
• W2034347824 countsByYear W20343478242022 @default.
• W2034347824 countsByYear W20343478242023 @default.
• W2034347824 crossrefType "journal-article" @default.
• W2034347824 hasAuthorship W2034347824A5000169747 @default.
• W2034347824 hasAuthorship W2034347824A5004383934 @default.
• W2034347824 hasAuthorship W2034347824A5004729412 @default.
• W2034347824 hasAuthorship W2034347824A5028915237 @default.
• W2034347824 hasAuthorship W2034347824A5049462378 @default.
• W2034347824 hasAuthorship W2034347824A5072924257 @default.
• W2034347824 hasBestOaLocation W20343478241 @default.
• W2034347824 hasConcept C104317684 @default.
• W2034347824 hasConcept C134792803 @default.
• W2034347824 hasConcept C150194340 @default.
• W2034347824 hasConcept C153911025 @default.
• W2034347824 hasConcept C187882448 @default.
• W2034347824 hasConcept C2779148672 @default.
• W2034347824 hasConcept C2779422922 @default.
• W2034347824 hasConcept C2779454504 @default.
• W2034347824 hasConcept C33922130 @default.
• W2034347824 hasConcept C4692481 @default.
• W2034347824 hasConcept C47698986 @default.
• W2034347824 hasConcept C48023723 @default.
• W2034347824 hasConcept C54355233 @default.
• W2034347824 hasConcept C55493867 @default.
• W2034347824 hasConcept C67905577 @default.
• W2034347824 hasConcept C86803240 @default.
• W2034347824 hasConceptScore W2034347824C104317684 @default.
• W2034347824 hasConceptScore W2034347824C134792803 @default.
• W2034347824 hasConceptScore W2034347824C150194340 @default.
• W2034347824 hasConceptScore W2034347824C153911025 @default.
• W2034347824 hasConceptScore W2034347824C187882448 @default.
• W2034347824 hasConceptScore W2034347824C2779148672 @default.
• W2034347824 hasConceptScore W2034347824C2779422922 @default.
• W2034347824 hasConceptScore W2034347824C2779454504 @default.
• W2034347824 hasConceptScore W2034347824C33922130 @default.
• W2034347824 hasConceptScore W2034347824C4692481 @default.
• W2034347824 hasConceptScore W2034347824C47698986 @default.
• W2034347824 hasConceptScore W2034347824C48023723 @default.
• W2034347824 hasConceptScore W2034347824C54355233 @default.
• W2034347824 hasConceptScore W2034347824C55493867 @default.
• W2034347824 hasConceptScore W2034347824C67905577 @default.
• W2034347824 hasConceptScore W2034347824C86803240 @default.
• W2034347824 hasIssue "1" @default.

• next