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• W2034355138 abstract "A structure-switching DNA optical biosensor for rapid on-site/in situ detection of heavy metal ions is reported. Mercury ions (Hg²⁺), highly toxic and ubiquitous pollutants, were selected as model target. In this system, fluorescence-labeled DNA containing T-T mismatch structure was introduced to bind with DNA probes immobilized onto the sensor surface. In the presence of Hg²⁺, some of the fluorescence-labeled DNAs bind with Hg²⁺ to form T-Hg²⁺-T complexes through the folding of themselves into a hairpin structure and dehybridization from the sensor surface, which leads to decrease in fluorescence signal. The total analysis time for a single sample was less than 10 min with detection limit of 1.2 nM. The rapid on-site/in situ determination of Hg²⁺ was readily performed in natural water. This sensing strategy can be extended in principle to other metal ions by substituting the T-Hg²⁺-T complexes with other specificity structures that selectively bind to other analytes." @default.
• W2034355138 created "2016-06-24" @default.
• W2034355138 creator A5008526861 @default.
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• W2034355138 date "2013-07-29" @default.
• W2034355138 modified "2023-10-17" @default.
• W2034355138 title "Rapid on-site/in-situ detection of heavy metal ions in environmental water using a structure-switching DNA optical biosensor" @default.
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• W2034355138 doi "https://doi.org/10.1038/srep02308" @default.
• W2034355138 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3725506" @default.
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