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- W2034360934 abstract "Interaction of endothelial-lineage cells with three-dimensional substrates was much less studied than that with flat culture surfaces. We investigated the in vitro attachment of both mature endothelial cells (ECs) and of less differentiated EC colony-forming cells to poly-ε-capro-lactone (PCL) fibers with diameters in 5-20 μm range ('scaffold microfibers', SMFs). We found that notwithstanding the poor intrinsic adhesiveness to PCL, both cell types completely wrapped the SMFs after long-term cultivation, thus attaining a cylindrical morphology. In this system, both EC types grew vigorously for more than a week and became increasingly more differentiated, as shown by multiplexed gene expression. Three-dimensional reconstructions from multiphoton confocal microscopy images using custom software showed that the filamentous (F) actin bundles took a conspicuous ring-like organization around the SMFs. Unlike the classical F-actin-containing stress fibers, these rings were not associated with either focal adhesions or intermediate filaments. We also demonstrated that plasma membrane boundaries adjacent to these circular cytoskeletal structures were tightly yet dynamically apposed to the SMFs, for which reason we suggest to call them 'actin grips'. In conclusion, we describe a particular form of F-actin assembly with relevance for cytoskeletal organization in response to biomaterials, for endothelial-specific cell behavior in vitro and in vivo, and for tissue engineering." @default.
- W2034360934 created "2016-06-24" @default.
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- W2034360934 date "2015-06-01" @default.
- W2034360934 modified "2023-09-23" @default.
- W2034360934 title "Actin grips: Circular actin-rich cytoskeletal structures that mediate the wrapping of polymeric microfibers by endothelial cells" @default.
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- W2034360934 cites W1972480801 @default.
- W2034360934 cites W1976860485 @default.
- W2034360934 cites W1983882645 @default.
- W2034360934 cites W1996989763 @default.
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- W2034360934 cites W2022363275 @default.
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- W2034360934 cites W2026742692 @default.
- W2034360934 cites W2028013007 @default.
- W2034360934 cites W2028376002 @default.
- W2034360934 cites W2030763568 @default.
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- W2034360934 cites W2039205173 @default.
- W2034360934 cites W2048366073 @default.
- W2034360934 cites W2049287425 @default.
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- W2034360934 cites W2065775451 @default.
- W2034360934 cites W2068523492 @default.
- W2034360934 cites W2074259144 @default.
- W2034360934 cites W2075755310 @default.
- W2034360934 cites W2078684403 @default.
- W2034360934 cites W2082013410 @default.
- W2034360934 cites W2082067280 @default.
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- W2034360934 cites W2085244040 @default.
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- W2034360934 cites W2103406964 @default.
- W2034360934 cites W2107144573 @default.
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- W2034360934 cites W2117535099 @default.
- W2034360934 cites W2120608193 @default.
- W2034360934 cites W2121621281 @default.
- W2034360934 cites W2123735680 @default.
- W2034360934 cites W2125062833 @default.
- W2034360934 cites W2125801586 @default.
- W2034360934 cites W2129962922 @default.
- W2034360934 cites W2137354732 @default.
- W2034360934 cites W2139027064 @default.
- W2034360934 cites W2139123746 @default.
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- W2034360934 doi "https://doi.org/10.1016/j.biomaterials.2015.02.034" @default.
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