FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034383732> ?p ?o ?g. }

• W2034383732 endingPage "852" @default.
• W2034383732 startingPage "842" @default.
• W2034383732 abstract "Nisin is an antimicrobial peptide used as food preservative. To gain some insights into the hypothesis that its bactericidal activity is due to the perturbation of the lipid fraction of the bacterial plasmic membrane, we have investigated the effect of nisin on model phosphatidylcholine (PC) membranes. We show that nisin affects the PC membrane permeability, and this perturbation is modulated by the lipid composition. Nisin-induced leakage from PC vesicles is inhibited by the presence of cholesterol. This inhibition is associated with the formation of a liquid ordered phase in the presence of cholesterol, which most likely reduces nisin affinity for the membrane. Conversely, phosphatidylglycerol (PG), an anionic lipid, promotes nisin-induced leakage, and this promotion is associated with an increased affinity of the peptide for the bilayer because nisin is a cationic peptide. When the electrostatic interactions are encouraged by the presence of 70 mol% PG in PC, the inhibitory effect of cholesterol is not observed anymore. Nisin drastically modifies the morphology of the dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) multilamellar dispersion without causing a significant change in the gel-to-liquid crystalline phase transition of the lipid. The morphological changes are observed from (31)P and (2)H NMR and cryo-electron microscopy. From the NMR point of view, the interactions giving rise to a broad signal (quadrupolar interactions and chemical shift anisotropy for (2)H NMR and (31)P NMR, respectively) are partly averaged out in the presence of nisin. This phenomenon is interpreted by the formation of curved lipid planes that lead to the lipid lateral diffusion occurring in the intermediate motional regime. By cryo-electron microscopy, large amorphous aggregates containing small dense globular particles are observed for samples quenched from 25 and 50 degrees C. Long thread-like structures are also observed in the fluid phase. A structural description of DPPC/nisin complex, consistent with the experimental observation, is proposed. The presence of 30 mol% cholesterol in DPPC completely inhibits the morphological changes induced by nisin. Therefore, it is concluded that nisin can significantly perturb PC bilayers from both the permeability and the structural points of view, and these perturbations are modulated by the lipidic species in the bilayer." @default.
• W2034383732 created "2016-06-24" @default.
• W2034383732 creator A5040038736 @default.
• W2034383732 creator A5058135197 @default.
• W2034383732 creator A5068959766 @default.
• W2034383732 date "1999-08-01" @default.
• W2034383732 modified "2023-10-10" @default.
• W2034383732 title "Characterization of Permeability and Morphological Perturbations Induced by Nisin on Phosphatidylcholine Membranes" @default.
• W2034383732 cites W1585996643 @default.
• W2034383732 cites W1969650220 @default.
• W2034383732 cites W1981466058 @default.
• W2034383732 cites W1993791807 @default.
• W2034383732 cites W1993927691 @default.
• W2034383732 cites W1994495883 @default.
• W2034383732 cites W1999481322 @default.
• W2034383732 cites W2008006633 @default.
• W2034383732 cites W2013105141 @default.
• W2034383732 cites W2016971745 @default.
• W2034383732 cites W2034191594 @default.
• W2034383732 cites W2035977517 @default.
• W2034383732 cites W2041132328 @default.
• W2034383732 cites W2051158595 @default.
• W2034383732 cites W2051732765 @default.
• W2034383732 cites W2055134616 @default.
• W2034383732 cites W2057901960 @default.
• W2034383732 cites W2061813654 @default.
• W2034383732 cites W2067129147 @default.
• W2034383732 cites W2075173556 @default.
• W2034383732 cites W2085183163 @default.
• W2034383732 cites W2087741554 @default.
• W2034383732 cites W2088998395 @default.
• W2034383732 cites W2089792637 @default.
• W2034383732 cites W2094297594 @default.
• W2034383732 cites W2099135211 @default.
• W2034383732 cites W2100277556 @default.
• W2034383732 cites W2100706403 @default.
• W2034383732 cites W2106378767 @default.
• W2034383732 cites W2119569975 @default.
• W2034383732 cites W2123661721 @default.
• W2034383732 cites W2151519197 @default.
• W2034383732 cites W2156478040 @default.
• W2034383732 cites W2160564346 @default.
• W2034383732 cites W2172156581 @default.
• W2034383732 cites W81206062 @default.
• W2034383732 doi "https://doi.org/10.1016/s0006-3495(99)76936-5" @default.
• W2034383732 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1300376" @default.
• W2034383732 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10423430" @default.
• W2034383732 hasPublicationYear "1999" @default.
• W2034383732 type Work @default.
• W2034383732 sameAs 2034383732 @default.
• W2034383732 citedByCount "66" @default.
• W2034383732 countsByYear W20343837322012 @default.
• W2034383732 countsByYear W20343837322014 @default.
• W2034383732 countsByYear W20343837322015 @default.
• W2034383732 countsByYear W20343837322016 @default.
• W2034383732 countsByYear W20343837322017 @default.
• W2034383732 countsByYear W20343837322018 @default.
• W2034383732 countsByYear W20343837322019 @default.
• W2034383732 countsByYear W20343837322020 @default.
• W2034383732 countsByYear W20343837322021 @default.
• W2034383732 countsByYear W20343837322022 @default.
• W2034383732 countsByYear W20343837322023 @default.
• W2034383732 crossrefType "journal-article" @default.
• W2034383732 hasAuthorship W2034383732A5040038736 @default.
• W2034383732 hasAuthorship W2034383732A5058135197 @default.
• W2034383732 hasAuthorship W2034383732A5068959766 @default.
• W2034383732 hasBestOaLocation W20343837321 @default.
• W2034383732 hasConcept C122398194 @default.
• W2034383732 hasConcept C125235067 @default.
• W2034383732 hasConcept C12554922 @default.
• W2034383732 hasConcept C130316041 @default.
• W2034383732 hasConcept C178790620 @default.
• W2034383732 hasConcept C185592680 @default.
• W2034383732 hasConcept C192157962 @default.
• W2034383732 hasConcept C2776330855 @default.
• W2034383732 hasConcept C2776634448 @default.
• W2034383732 hasConcept C2778457984 @default.
• W2034383732 hasConcept C2778918659 @default.
• W2034383732 hasConcept C2780183148 @default.
• W2034383732 hasConcept C2780726299 @default.
• W2034383732 hasConcept C39944091 @default.
• W2034383732 hasConcept C41625074 @default.
• W2034383732 hasConcept C4937899 @default.
• W2034383732 hasConcept C55493867 @default.
• W2034383732 hasConcept C86803240 @default.
• W2034383732 hasConceptScore W2034383732C122398194 @default.
• W2034383732 hasConceptScore W2034383732C125235067 @default.
• W2034383732 hasConceptScore W2034383732C12554922 @default.
• W2034383732 hasConceptScore W2034383732C130316041 @default.
• W2034383732 hasConceptScore W2034383732C178790620 @default.
• W2034383732 hasConceptScore W2034383732C185592680 @default.
• W2034383732 hasConceptScore W2034383732C192157962 @default.
• W2034383732 hasConceptScore W2034383732C2776330855 @default.
• W2034383732 hasConceptScore W2034383732C2776634448 @default.
• W2034383732 hasConceptScore W2034383732C2778457984 @default.
• W2034383732 hasConceptScore W2034383732C2778918659 @default.
• W2034383732 hasConceptScore W2034383732C2780183148 @default.
• W2034383732 hasConceptScore W2034383732C2780726299 @default.

• next