Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034385969> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2034385969 endingPage "6605" @default.
- W2034385969 startingPage "6596" @default.
- W2034385969 abstract "We have determined the key regions for protein foldability by creating multiple crossover libraries from two proteins that share similar fold but have low sequence identity and differ significantly in stability. One protein is the propeptide of a serine protease, subtilisin BPN', and the other is Pleurotus ostreatus proteinase A inhibitor 1 (POIA1). The propeptide has a compact structure when complexed with subtilisin but is unstructured when isolated, whereas POIA1 takes a stable structure. We selected four of the conserved amino acid residues for the boundaries of crossover sites and utilized these residues to make same cohesive-ends to assemble synthetic DNA fragments. Each segment has one or two secondary structure units, and the interchange of these structural elements produces 32 (= 2(5)) combinations, including the propeptide and POIA1. The stability of these mutants was first screened by formation of turbid zones on skim milk plates containing subtilisin BPN'. It was shown that six variants were foldable and structural units necessary for folding were identified. Further fragmentation and recombination of these mutants (the multisection method) revealed that two interactions between secondary structures are important; one is interaction between the loop-alpha1 and beta2-turn-beta3, and the other is hydrophobic interaction between the adjoining beta1 and beta4 strands. We were also able to specify the significant amino acid combinations for tolerance to proteolysis. These combinatorial methods not only elucidate how domains can be interchanged to make the whole protein foldable but also extract essential regions for the function, which is correlated with the instability of the molecule." @default.
- W2034385969 created "2016-06-24" @default.
- W2034385969 creator A5033895001 @default.
- W2034385969 creator A5068769101 @default.
- W2034385969 date "2004-06-01" @default.
- W2034385969 modified "2023-09-24" @default.
- W2034385969 title "Key Elements for Protein Foldability Revealed by a Combinatorial Approach among Similarly Folded but Distantly Related Proteins†" @default.
- W2034385969 cites W1591438776 @default.
- W2034385969 cites W1612480077 @default.
- W2034385969 cites W1950598798 @default.
- W2034385969 cites W1977101756 @default.
- W2034385969 cites W1990878089 @default.
- W2034385969 cites W2001952758 @default.
- W2034385969 cites W2002195659 @default.
- W2034385969 cites W2015611327 @default.
- W2034385969 cites W2046460151 @default.
- W2034385969 cites W2092320171 @default.
- W2034385969 cites W2146106056 @default.
- W2034385969 cites W2414435754 @default.
- W2034385969 cites W2949340687 @default.
- W2034385969 cites W2951476220 @default.
- W2034385969 doi "https://doi.org/10.1021/bi0302402" @default.
- W2034385969 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15157092" @default.
- W2034385969 hasPublicationYear "2004" @default.
- W2034385969 type Work @default.
- W2034385969 sameAs 2034385969 @default.
- W2034385969 citedByCount "4" @default.
- W2034385969 crossrefType "journal-article" @default.
- W2034385969 hasAuthorship W2034385969A5033895001 @default.
- W2034385969 hasAuthorship W2034385969A5068769101 @default.
- W2034385969 hasConcept C104317684 @default.
- W2034385969 hasConcept C143065580 @default.
- W2034385969 hasConcept C147816474 @default.
- W2034385969 hasConcept C167625842 @default.
- W2034385969 hasConcept C181199279 @default.
- W2034385969 hasConcept C182220744 @default.
- W2034385969 hasConcept C185592680 @default.
- W2034385969 hasConcept C204328495 @default.
- W2034385969 hasConcept C2776714187 @default.
- W2034385969 hasConcept C2779811187 @default.
- W2034385969 hasConcept C2781307694 @default.
- W2034385969 hasConcept C47701112 @default.
- W2034385969 hasConcept C515207424 @default.
- W2034385969 hasConcept C55493867 @default.
- W2034385969 hasConcept C62614982 @default.
- W2034385969 hasConcept C7860815 @default.
- W2034385969 hasConcept C86803240 @default.
- W2034385969 hasConceptScore W2034385969C104317684 @default.
- W2034385969 hasConceptScore W2034385969C143065580 @default.
- W2034385969 hasConceptScore W2034385969C147816474 @default.
- W2034385969 hasConceptScore W2034385969C167625842 @default.
- W2034385969 hasConceptScore W2034385969C181199279 @default.
- W2034385969 hasConceptScore W2034385969C182220744 @default.
- W2034385969 hasConceptScore W2034385969C185592680 @default.
- W2034385969 hasConceptScore W2034385969C204328495 @default.
- W2034385969 hasConceptScore W2034385969C2776714187 @default.
- W2034385969 hasConceptScore W2034385969C2779811187 @default.
- W2034385969 hasConceptScore W2034385969C2781307694 @default.
- W2034385969 hasConceptScore W2034385969C47701112 @default.
- W2034385969 hasConceptScore W2034385969C515207424 @default.
- W2034385969 hasConceptScore W2034385969C55493867 @default.
- W2034385969 hasConceptScore W2034385969C62614982 @default.
- W2034385969 hasConceptScore W2034385969C7860815 @default.
- W2034385969 hasConceptScore W2034385969C86803240 @default.
- W2034385969 hasIssue "21" @default.
- W2034385969 hasLocation W20343859691 @default.
- W2034385969 hasLocation W20343859692 @default.
- W2034385969 hasOpenAccess W2034385969 @default.
- W2034385969 hasPrimaryLocation W20343859691 @default.
- W2034385969 hasRelatedWork W1987958571 @default.
- W2034385969 hasRelatedWork W2026960302 @default.
- W2034385969 hasRelatedWork W2065384285 @default.
- W2034385969 hasRelatedWork W2094275533 @default.
- W2034385969 hasRelatedWork W2098450020 @default.
- W2034385969 hasRelatedWork W2114609271 @default.
- W2034385969 hasRelatedWork W2139138166 @default.
- W2034385969 hasRelatedWork W2509227587 @default.
- W2034385969 hasRelatedWork W2993740618 @default.
- W2034385969 hasRelatedWork W2106807130 @default.
- W2034385969 hasVolume "43" @default.
- W2034385969 isParatext "false" @default.
- W2034385969 isRetracted "false" @default.
- W2034385969 magId "2034385969" @default.
- W2034385969 workType "article" @default.