FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034386905> ?p ?o ?g. }

• W2034386905 endingPage "19248" @default.
• W2034386905 startingPage "19243" @default.
• W2034386905 abstract "High-throughput screening and optimization experiments are critical to a number of fields, including chemistry and structural and molecular biology. The separation of these two steps may introduce false negatives and a time delay between initial screening and subsequent optimization. Although a hybrid method combining both steps may address these problems, miniaturization is required to minimize sample consumption. This article reports a “hybrid” droplet-based microfluidic approach that combines the steps of screening and optimization into one simple experiment and uses nanoliter-sized plugs to minimize sample consumption. Many distinct reagents were sequentially introduced as ≈140-nl plugs into a microfluidic device and combined with a substrate and a diluting buffer. Tests were conducted in ≈10-nl plugs containing different concentrations of a reagent. Methods were developed to form plugs of controlled concentrations, index concentrations, and incubate thousands of plugs inexpensively and without evaporation. To validate the hybrid method and demonstrate its applicability to challenging problems, crystallization of model membrane proteins and handling of solutions of detergents and viscous precipitants were demonstrated. By using 10 μl of protein solution, ≈1,300 crystallization trials were set up within 20 min by one researcher. This method was compatible with growth, manipulation, and extraction of high-quality crystals of membrane proteins, demonstrated by obtaining high-resolution diffraction images and solving a crystal structure. This robust method requires inexpensive equipment and supplies, should be especially suitable for use in individual laboratories, and could find applications in a number of areas that require chemical, biochemical, and biological screening and optimization." @default.
• W2034386905 created "2016-06-24" @default.
• W2034386905 creator A5004700629 @default.
• W2034386905 creator A5008211866 @default.
• W2034386905 creator A5040072389 @default.
• W2034386905 creator A5053853885 @default.
• W2034386905 creator A5060148109 @default.
• W2034386905 creator A5082865512 @default.
• W2034386905 creator A5091057740 @default.
• W2034386905 date "2006-12-19" @default.
• W2034386905 modified "2023-10-04" @default.
• W2034386905 title "Nanoliter microfluidic hybrid method for simultaneous screening and optimization validated with crystallization of membrane proteins" @default.
• W2034386905 cites W1500418255 @default.
• W2034386905 cites W1560890112 @default.
• W2034386905 cites W1637799068 @default.
• W2034386905 cites W1964593962 @default.
• W2034386905 cites W1967193064 @default.
• W2034386905 cites W1967567333 @default.
• W2034386905 cites W1974247808 @default.
• W2034386905 cites W1975330940 @default.
• W2034386905 cites W1976275229 @default.
• W2034386905 cites W1995087505 @default.
• W2034386905 cites W1995089537 @default.
• W2034386905 cites W1995496955 @default.
• W2034386905 cites W1995980985 @default.
• W2034386905 cites W2005496553 @default.
• W2034386905 cites W2014088797 @default.
• W2034386905 cites W2014895510 @default.
• W2034386905 cites W2015982647 @default.
• W2034386905 cites W2020068704 @default.
• W2034386905 cites W2027148545 @default.
• W2034386905 cites W2028894110 @default.
• W2034386905 cites W2032734987 @default.
• W2034386905 cites W2041416107 @default.
• W2034386905 cites W2046413244 @default.
• W2034386905 cites W2051021490 @default.
• W2034386905 cites W2059883330 @default.
• W2034386905 cites W2077616900 @default.
• W2034386905 cites W2078863041 @default.
• W2034386905 cites W2079231898 @default.
• W2034386905 cites W2084292270 @default.
• W2034386905 cites W2084311198 @default.
• W2034386905 cites W2084914807 @default.
• W2034386905 cites W2089320738 @default.
• W2034386905 cites W2101475717 @default.
• W2034386905 cites W2103169680 @default.
• W2034386905 cites W2116117181 @default.
• W2034386905 cites W2128879247 @default.
• W2034386905 cites W2129848443 @default.
• W2034386905 cites W2141923642 @default.
• W2034386905 cites W2147869016 @default.
• W2034386905 cites W2149881082 @default.
• W2034386905 cites W2151248200 @default.
• W2034386905 cites W2151336130 @default.
• W2034386905 cites W2160569176 @default.
• W2034386905 cites W2161774073 @default.
• W2034386905 cites W2167093616 @default.
• W2034386905 doi "https://doi.org/10.1073/pnas.0607502103" @default.
• W2034386905 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1748211" @default.
• W2034386905 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17159147" @default.
• W2034386905 hasPublicationYear "2006" @default.
• W2034386905 type Work @default.
• W2034386905 sameAs 2034386905 @default.
• W2034386905 citedByCount "228" @default.
• W2034386905 countsByYear W20343869052012 @default.
• W2034386905 countsByYear W20343869052013 @default.
• W2034386905 countsByYear W20343869052014 @default.
• W2034386905 countsByYear W20343869052015 @default.
• W2034386905 countsByYear W20343869052016 @default.
• W2034386905 countsByYear W20343869052017 @default.
• W2034386905 countsByYear W20343869052018 @default.
• W2034386905 countsByYear W20343869052019 @default.
• W2034386905 countsByYear W20343869052020 @default.
• W2034386905 countsByYear W20343869052021 @default.
• W2034386905 countsByYear W20343869052022 @default.
• W2034386905 countsByYear W20343869052023 @default.
• W2034386905 crossrefType "journal-article" @default.
• W2034386905 hasAuthorship W2034386905A5004700629 @default.
• W2034386905 hasAuthorship W2034386905A5008211866 @default.
• W2034386905 hasAuthorship W2034386905A5040072389 @default.
• W2034386905 hasAuthorship W2034386905A5053853885 @default.
• W2034386905 hasAuthorship W2034386905A5060148109 @default.
• W2034386905 hasAuthorship W2034386905A5082865512 @default.
• W2034386905 hasAuthorship W2034386905A5091057740 @default.
• W2034386905 hasBestOaLocation W20343869051 @default.
• W2034386905 hasConcept C127413603 @default.
• W2034386905 hasConcept C147789679 @default.
• W2034386905 hasConcept C153064111 @default.
• W2034386905 hasConcept C157764524 @default.
• W2034386905 hasConcept C171250308 @default.
• W2034386905 hasConcept C185592680 @default.
• W2034386905 hasConcept C186060115 @default.
• W2034386905 hasConcept C192562407 @default.
• W2034386905 hasConcept C203036418 @default.
• W2034386905 hasConcept C21880701 @default.
• W2034386905 hasConcept C40875361 @default.
• W2034386905 hasConcept C41008148 @default.
• W2034386905 hasConcept C41625074 @default.

• next