Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034388289> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2034388289 abstract "Since the removal of only one of the two nucleic acids, ribonucleic and deoxyribonucleic, is often necessary in biological work, the optimum conditions for the specific removal of either nucleic acid by the corresponding enzyme were investigated. The specific removal of nucleic acids by enzyme treatment was tested by toluidine blue staining and by quantitative study of the radioautographic reactions produced by the labelled deoxyribonucleic acid and ribonucleic acid appearing in tissues of a cytidine-H 3 injected mouse. For the removal of ribonucleic acid by ribonuclease, the tissues must be fixed in freshly prepared Carnoy's solution for 24 hours, as a shorter fixation permits the loss of ribonucleic acid from control tissue sections. Ribonuclease extraction (1 mg crystallized salt-free Worthington ribonuclease per 1 ml of distilled water) is carried out for 4 hours at 40°C. For the removal of deoxyribonucleic acid by deoxyribonuclease, it was also necessary to use 24 hour fixation in freshly prepared Carnoy. A high concentration of magnesium was found to be necessary in the incubation solution (0.05 mg crystallized Worthington deoxyribonuclease per 1 ml of 0.2 M Mg-containing Gomori's Tris maleic acid buffer solution). The incubation was carried out for 24 hours at 37°C. The toluidine blue stained basophilic material and the radioautographic reactions in the tissues of the cytidine-H 3 injected mouse were lost following a double treatment with ribonuclease and deoxyribonuclease or by hot trichloracetic acid extraction. Therefore all the reactions appearing over the histological tissue sections are due to the tritium incorporated into deoxyribonucleic acid and ribonucleic acid. The basophilic material from cytoplasm and nucleolus was removed specifically and completely by ribonuclease treatment. The deoxyribonuclease treatment removed nuclear basophilia exclusively. However, controls of ribonuclease or deoxyribonuclease treatment showed no loss of basophilia, except on occasion in the pancreas. The radioautographic reactions found in the mouse tissues 24 hours after cytidine-H 3 injection were exclusively localized over the nuclear basophilia of some nuclei after ribonuclease treatment, but were largely over the cytoplasmic basophilia after deoxyribonuclease treatment. Quantitative radioautographic study showed that either enzyme treatment removed its specific substrate completely and exclusively without loss in control preparations." @default.
- W2034388289 created "2016-06-24" @default.
- W2034388289 creator A5031710163 @default.
- W2034388289 date "1962-03-01" @default.
- W2034388289 modified "2023-10-10" @default.
- W2034388289 title "IMPROVED TECHNIQUES FOR THE ENZYMATIC EXTRACTION OF NUCLEIC ACIDS FROM TISSUE SECTIONS" @default.
- W2034388289 doi "https://doi.org/10.1177/10.2.204" @default.
- W2034388289 hasPublicationYear "1962" @default.
- W2034388289 type Work @default.
- W2034388289 sameAs 2034388289 @default.
- W2034388289 citedByCount "59" @default.
- W2034388289 crossrefType "journal-article" @default.
- W2034388289 hasAuthorship W2034388289A5031710163 @default.
- W2034388289 hasBestOaLocation W20343882891 @default.
- W2034388289 hasConcept C104317684 @default.
- W2034388289 hasConcept C10879258 @default.
- W2034388289 hasConcept C111921641 @default.
- W2034388289 hasConcept C147789679 @default.
- W2034388289 hasConcept C181199279 @default.
- W2034388289 hasConcept C185592680 @default.
- W2034388289 hasConcept C24107716 @default.
- W2034388289 hasConcept C2778659741 @default.
- W2034388289 hasConcept C2778976237 @default.
- W2034388289 hasConcept C2779262481 @default.
- W2034388289 hasConcept C2779429693 @default.
- W2034388289 hasConcept C2780058249 @default.
- W2034388289 hasConcept C2781184954 @default.
- W2034388289 hasConcept C43617362 @default.
- W2034388289 hasConcept C54355233 @default.
- W2034388289 hasConcept C552990157 @default.
- W2034388289 hasConcept C55493867 @default.
- W2034388289 hasConcept C67705224 @default.
- W2034388289 hasConcept C74864618 @default.
- W2034388289 hasConcept C86803240 @default.
- W2034388289 hasConceptScore W2034388289C104317684 @default.
- W2034388289 hasConceptScore W2034388289C10879258 @default.
- W2034388289 hasConceptScore W2034388289C111921641 @default.
- W2034388289 hasConceptScore W2034388289C147789679 @default.
- W2034388289 hasConceptScore W2034388289C181199279 @default.
- W2034388289 hasConceptScore W2034388289C185592680 @default.
- W2034388289 hasConceptScore W2034388289C24107716 @default.
- W2034388289 hasConceptScore W2034388289C2778659741 @default.
- W2034388289 hasConceptScore W2034388289C2778976237 @default.
- W2034388289 hasConceptScore W2034388289C2779262481 @default.
- W2034388289 hasConceptScore W2034388289C2779429693 @default.
- W2034388289 hasConceptScore W2034388289C2780058249 @default.
- W2034388289 hasConceptScore W2034388289C2781184954 @default.
- W2034388289 hasConceptScore W2034388289C43617362 @default.
- W2034388289 hasConceptScore W2034388289C54355233 @default.
- W2034388289 hasConceptScore W2034388289C552990157 @default.
- W2034388289 hasConceptScore W2034388289C55493867 @default.
- W2034388289 hasConceptScore W2034388289C67705224 @default.
- W2034388289 hasConceptScore W2034388289C74864618 @default.
- W2034388289 hasConceptScore W2034388289C86803240 @default.
- W2034388289 hasLocation W20343882891 @default.
- W2034388289 hasOpenAccess W2034388289 @default.
- W2034388289 hasPrimaryLocation W20343882891 @default.
- W2034388289 hasRelatedWork W1855731855 @default.
- W2034388289 hasRelatedWork W1971350783 @default.
- W2034388289 hasRelatedWork W1973225384 @default.
- W2034388289 hasRelatedWork W1975322570 @default.
- W2034388289 hasRelatedWork W1990976908 @default.
- W2034388289 hasRelatedWork W1993164553 @default.
- W2034388289 hasRelatedWork W1993273127 @default.
- W2034388289 hasRelatedWork W1993291120 @default.
- W2034388289 hasRelatedWork W1994311035 @default.
- W2034388289 hasRelatedWork W2012852567 @default.
- W2034388289 hasRelatedWork W2013434091 @default.
- W2034388289 hasRelatedWork W2016029001 @default.
- W2034388289 hasRelatedWork W2019262596 @default.
- W2034388289 hasRelatedWork W2025594687 @default.
- W2034388289 hasRelatedWork W2028220949 @default.
- W2034388289 hasRelatedWork W2032258056 @default.
- W2034388289 hasRelatedWork W2073139781 @default.
- W2034388289 hasRelatedWork W2127896362 @default.
- W2034388289 hasRelatedWork W2334521023 @default.
- W2034388289 hasRelatedWork W2768161102 @default.
- W2034388289 isParatext "false" @default.
- W2034388289 isRetracted "false" @default.
- W2034388289 magId "2034388289" @default.
- W2034388289 workType "article" @default.