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- W2034392008 abstract "Abstract Free fatty acids from fish oil were prepared by saponification of menhaden oil. The resulting mixture of fatty acids contained ca . 15% eicosapentaenoic acid (EPA) and 10% docosahexaenoic acid (DHA), together with other saturated and monounsaturated fatty acids. Four commercial lipases (PS from Pseudomonas cepacia , G from Penicillium camemberti , L2 from Candida antarctica fraction B, and L9 from Mucor miehei ) were tested for their ability to catalyze the esterification of glycerol with a mixture of free fatty acids derived from saponified menhaden oil, to which 20% (w/w) conjugated linoleic acid had been added. The mixtures were incubated at 40°C for 48h. The ultimate extent of the esterification reaction (60%) was similar for three of the four lipases studied. Lipase PS produced triacylglycerols at the fastest rate. Lipase G differed from the other three lipases in terms of effecting a much slower reaction rate. In addition, the rate of incorporation of omega‐3 fatty acids when mediated by lipase G was slower than the rates of incorporation of other fatty acids present in the reaction mixture. With respect to fatty acid specificities, lipases PS and L9 showed appreciable discrimination against esterification of EPA and DHA, respectively, while lipase L2 exhibited similar activity for all fatty acids present in the reaction mixture. The positional distribution of the various fatty acids between the sn ‐1,3 and sn ‐2 positions on the glycerol backbone was also determined." @default.
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- W2034392008 date "2001-11-01" @default.
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- W2034392008 title "Esterification of glycerol with conjugated linoleic acid and long-chain fatty acids from fish oil" @default.
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- W2034392008 doi "https://doi.org/10.1007/s11746-001-0395-8" @default.
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