FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034406220> ?p ?o ?g. }

• W2034406220 endingPage "61" @default.
• W2034406220 startingPage "53" @default.
• W2034406220 abstract "The rapid and direct quantification of Campylobacter spp. in complex substrates like feces or environmental samples is crucial to facilitate epidemiological studies on Campylobacter in pig production systems. We developed a real-time PCR assay for detecting and quantifying Campylobacter spp. directly in pig feces with the use of an internal control. Campylobacter spp. and Yersinia ruckeri primers–probes sets were designed and checked for specificity with diverse Campylobacter, related organisms, and other bacterial pathogens before being used in field samples. The quantification of Campylobacter spp. by the real-time PCR then was realized on 531 fecal samples obtained from experimentally and naturally infected pigs; the numeration of Campylobacter on Karmali plate was done in parallel. Yersinia ruckeri, used as bacterial internal control, was added to the samples before DNA extraction to control DNA-extraction and PCR-amplification. The sensitivity of the PCR assay was 10 genome copies. The established Campylobacter real-time PCR assay showed a 7-log-wide linear dynamic range of quantification (R2 = 0.99) with a detection limit of 200 Colony Forming Units of Campylobacter per gram of feces. A high correlation was found between the results obtained by real-time PCR and those by culture at both qualitative and quantitative levels. Moreover, DNA extraction followed by real-time PCR reduced the time needed for analysis to a few hours (within a working day). In conclusion, the real-time PCR developed in this study provides new tools for further epidemiological surveys to investigate the carriage and excretion of Campylobacter by pigs." @default.
• W2034406220 created "2016-06-24" @default.
• W2034406220 creator A5002596406 @default.
• W2034406220 creator A5016372992 @default.
• W2034406220 creator A5035741382 @default.
• W2034406220 creator A5046628385 @default.
• W2034406220 creator A5058896027 @default.
• W2034406220 creator A5066501527 @default.
• W2034406220 creator A5083223915 @default.
• W2034406220 date "2011-04-01" @default.
• W2034406220 modified "2023-10-18" @default.
• W2034406220 title "Quantification of Campylobacter spp. in pig feces by direct real-time PCR with an internal control of extraction and amplification" @default.
• W2034406220 cites W1574727116 @default.
• W2034406220 cites W1808254916 @default.
• W2034406220 cites W1882048989 @default.
• W2034406220 cites W1967536176 @default.
• W2034406220 cites W1969043085 @default.
• W2034406220 cites W1979619587 @default.
• W2034406220 cites W1984409531 @default.
• W2034406220 cites W1993402762 @default.
• W2034406220 cites W1996331174 @default.
• W2034406220 cites W2002168266 @default.
• W2034406220 cites W2006985658 @default.
• W2034406220 cites W2011754273 @default.
• W2034406220 cites W2020320943 @default.
• W2034406220 cites W2044957727 @default.
• W2034406220 cites W2050921222 @default.
• W2034406220 cites W2054038506 @default.
• W2034406220 cites W2059657252 @default.
• W2034406220 cites W2066485878 @default.
• W2034406220 cites W2070581349 @default.
• W2034406220 cites W2070952647 @default.
• W2034406220 cites W2083428458 @default.
• W2034406220 cites W2088668369 @default.
• W2034406220 cites W2094048328 @default.
• W2034406220 cites W2096755902 @default.
• W2034406220 cites W2096859880 @default.
• W2034406220 cites W2098991925 @default.
• W2034406220 cites W2099084353 @default.
• W2034406220 cites W2101960580 @default.
• W2034406220 cites W2103762229 @default.
• W2034406220 cites W2107508284 @default.
• W2034406220 cites W2108322580 @default.
• W2034406220 cites W2114702971 @default.
• W2034406220 cites W2114773536 @default.
• W2034406220 cites W2125934787 @default.
• W2034406220 cites W2130481903 @default.
• W2034406220 cites W2141403362 @default.
• W2034406220 cites W2146415849 @default.
• W2034406220 cites W2151500312 @default.
• W2034406220 cites W2151614941 @default.
• W2034406220 cites W2153875981 @default.
• W2034406220 cites W2156786954 @default.
• W2034406220 cites W2156859060 @default.
• W2034406220 cites W2158413356 @default.
• W2034406220 cites W2166422451 @default.
• W2034406220 cites W2248996783 @default.
• W2034406220 cites W4232109339 @default.
• W2034406220 doi "https://doi.org/10.1016/j.mimet.2011.01.013" @default.
• W2034406220 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21277341" @default.
• W2034406220 hasPublicationYear "2011" @default.
• W2034406220 type Work @default.
• W2034406220 sameAs 2034406220 @default.
• W2034406220 citedByCount "27" @default.
• W2034406220 countsByYear W20344062202012 @default.
• W2034406220 countsByYear W20344062202013 @default.
• W2034406220 countsByYear W20344062202014 @default.
• W2034406220 countsByYear W20344062202015 @default.
• W2034406220 countsByYear W20344062202016 @default.
• W2034406220 countsByYear W20344062202017 @default.
• W2034406220 countsByYear W20344062202018 @default.
• W2034406220 countsByYear W20344062202019 @default.
• W2034406220 countsByYear W20344062202020 @default.
• W2034406220 countsByYear W20344062202021 @default.
• W2034406220 countsByYear W20344062202022 @default.
• W2034406220 crossrefType "journal-article" @default.
• W2034406220 hasAuthorship W2034406220A5002596406 @default.
• W2034406220 hasAuthorship W2034406220A5016372992 @default.
• W2034406220 hasAuthorship W2034406220A5035741382 @default.
• W2034406220 hasAuthorship W2034406220A5046628385 @default.
• W2034406220 hasAuthorship W2034406220A5058896027 @default.
• W2034406220 hasAuthorship W2034406220A5066501527 @default.
• W2034406220 hasAuthorship W2034406220A5083223915 @default.
• W2034406220 hasConcept C104317684 @default.
• W2034406220 hasConcept C2776255393 @default.
• W2034406220 hasConcept C2776957140 @default.
• W2034406220 hasConcept C2779910956 @default.
• W2034406220 hasConcept C48023723 @default.
• W2034406220 hasConcept C49105822 @default.
• W2034406220 hasConcept C523546767 @default.
• W2034406220 hasConcept C54355233 @default.
• W2034406220 hasConcept C61716771 @default.
• W2034406220 hasConcept C78063203 @default.
• W2034406220 hasConcept C86803240 @default.
• W2034406220 hasConcept C89423630 @default.
• W2034406220 hasConceptScore W2034406220C104317684 @default.
• W2034406220 hasConceptScore W2034406220C2776255393 @default.
• W2034406220 hasConceptScore W2034406220C2776957140 @default.

• next