FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034437094> ?p ?o ?g. }

• W2034437094 endingPage "1072" @default.
• W2034437094 startingPage "1061" @default.
• W2034437094 abstract "Spermatocytes normally sustain many meiotically induced double-strand DNA breaks (DSBs) early in meiotic prophase; in autosomal chromatin, these are repaired by initiation of meiotic homologous-recombination processes. Little is known about how spermatocytes respond to environmentally induced DNA damage after recombination-related DSBs have been repaired. The experiments described here tested the hypothesis that, even though actively completing meiotic recombination, pachytene spermatocytes cultured in the absence of testicular somatic cells initiate appropriate chromatin remodeling and cell-cycle responses to environmentally induced DNA damage. Two DNA-damaging agents were employed for in vitro treatment of pachytene spermatocytes: γ-irradiation and etoposide, a topoisomerase II (TOP2) inhibitor that results in persistent unligated DSBs. Chromatin modifications associated with DSBs were monitored after exposure by labeling surface-spread chromatin with antibodies against RAD51 (which recognizes DSBs) and the phosphorylated variant of histone H2AFX (herein designated by its commonly used symbol, H2AX), γH2AX (which modifies chromatin associated with DSBs). Both γH2AX and RAD51 were rapidly recruited to irradiation- or etoposide-damaged chromatin. These chromatin modifications imply that spermatocytes recruit active DNA damage responses, even after recombination is substantially completed. Furthermore, irradiation-induced DNA damage inhibited okadaic acid-induced progression of spermatocytes from meiotic prophase to metaphase I (MI), implying efficacy of DNA damage checkpoint mechanisms. Apoptotic responses of spermatocytes with DNA damage differed, with an increase in frequency of early apoptotic spermatocytes after etoposide treatment, but not following irradiation. Taken together, these results demonstrate modification of pachytene spermatocyte chromatin and inhibition of meiotic progress after DNA damage by mechanisms that may ensure gametic genetic integrity. Mol. Reprod. Dev. 1061–1072, 2006. © 2006 Wiley-Liss, Inc." @default.
• W2034437094 created "2016-06-24" @default.
• W2034437094 creator A5005911983 @default.
• W2034437094 creator A5066865482 @default.
• W2034437094 date "2006-01-01" @default.
• W2034437094 modified "2023-09-25" @default.
• W2034437094 title "Spermatocyte responses in vitro to induced DNA damage" @default.
• W2034437094 cites W1489657805 @default.
• W2034437094 cites W1497633776 @default.
• W2034437094 cites W1498525243 @default.
• W2034437094 cites W1515564094 @default.
• W2034437094 cites W1529934215 @default.
• W2034437094 cites W1535454769 @default.
• W2034437094 cites W1566821222 @default.
• W2034437094 cites W1606401780 @default.
• W2034437094 cites W1660787335 @default.
• W2034437094 cites W1939711593 @default.
• W2034437094 cites W1964059076 @default.
• W2034437094 cites W1967972679 @default.
• W2034437094 cites W1968096672 @default.
• W2034437094 cites W1970551701 @default.
• W2034437094 cites W1971771310 @default.
• W2034437094 cites W1971887411 @default.
• W2034437094 cites W1977932741 @default.
• W2034437094 cites W1979457256 @default.
• W2034437094 cites W1990699200 @default.
• W2034437094 cites W1997157839 @default.
• W2034437094 cites W1999483579 @default.
• W2034437094 cites W2002292682 @default.
• W2034437094 cites W2003023259 @default.
• W2034437094 cites W2004008931 @default.
• W2034437094 cites W2007306633 @default.
• W2034437094 cites W2010465993 @default.
• W2034437094 cites W2014722055 @default.
• W2034437094 cites W2019405732 @default.
• W2034437094 cites W2019825147 @default.
• W2034437094 cites W2024116210 @default.
• W2034437094 cites W2025818802 @default.
• W2034437094 cites W2042119456 @default.
• W2034437094 cites W2047833120 @default.
• W2034437094 cites W2048660857 @default.
• W2034437094 cites W2049365893 @default.
• W2034437094 cites W2049858779 @default.
• W2034437094 cites W2052115030 @default.
• W2034437094 cites W2054305990 @default.
• W2034437094 cites W2055458870 @default.
• W2034437094 cites W2056508303 @default.
• W2034437094 cites W2057358681 @default.
• W2034437094 cites W2057658557 @default.
• W2034437094 cites W2060453248 @default.
• W2034437094 cites W2065022250 @default.
• W2034437094 cites W2073991271 @default.
• W2034437094 cites W2078994441 @default.
• W2034437094 cites W2079755465 @default.
• W2034437094 cites W2084969545 @default.
• W2034437094 cites W2086932293 @default.
• W2034437094 cites W2104749119 @default.
• W2034437094 cites W2109456034 @default.
• W2034437094 cites W2111279302 @default.
• W2034437094 cites W2115967293 @default.
• W2034437094 cites W2116537939 @default.
• W2034437094 cites W2122050600 @default.
• W2034437094 cites W2126428509 @default.
• W2034437094 cites W2126442673 @default.
• W2034437094 cites W2128680272 @default.
• W2034437094 cites W2136779659 @default.
• W2034437094 cites W2140698430 @default.
• W2034437094 cites W2145621949 @default.
• W2034437094 cites W2145718057 @default.
• W2034437094 cites W2147672884 @default.
• W2034437094 cites W2149342162 @default.
• W2034437094 cites W2153671974 @default.
• W2034437094 cites W2153807168 @default.
• W2034437094 cites W2163011434 @default.
• W2034437094 cites W2163550320 @default.
• W2034437094 cites W2286297234 @default.
• W2034437094 cites W2322667628 @default.
• W2034437094 cites W2332617654 @default.
• W2034437094 cites W2346702349 @default.
• W2034437094 doi "https://doi.org/10.1002/mrd.20508" @default.
• W2034437094 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16700071" @default.
• W2034437094 hasPublicationYear "2006" @default.
• W2034437094 type Work @default.
• W2034437094 sameAs 2034437094 @default.
• W2034437094 citedByCount "22" @default.
• W2034437094 countsByYear W20344370942012 @default.
• W2034437094 countsByYear W20344370942013 @default.
• W2034437094 countsByYear W20344370942015 @default.
• W2034437094 countsByYear W20344370942018 @default.
• W2034437094 countsByYear W20344370942019 @default.
• W2034437094 countsByYear W20344370942020 @default.
• W2034437094 countsByYear W20344370942022 @default.
• W2034437094 crossrefType "journal-article" @default.
• W2034437094 hasAuthorship W2034437094A5005911983 @default.
• W2034437094 hasAuthorship W2034437094A5066865482 @default.
• W2034437094 hasConcept C102744134 @default.
• W2034437094 hasConcept C104317684 @default.
• W2034437094 hasConcept C134935766 @default.

• next