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- W2034451538 abstract "The present study was conducted to determine the essential DNA sequences required for the transcription of the human phospholipid transfer protein gene. Truncation studies revealed that DNA sequences between −230 and −159, particularly those at the upstream region, were responsible for the full promoter activity. This region was able to compete with AP-2 and GRE oligonucloetides for the binding to HepG2 cell nuclear extract as shown by gel mobility shift assay. Further analysis, using site-directed mutagenesis, indicated that DNA sequences identical to Sp1 and highly homologous to GRE and Ap-2 consensus sequences were essential for the transcription. These findings support the concept that several elements, spread over the entire functional promoter, synergistically drive the basal transcription." @default.
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- W2034451538 date "1997-03-01" @default.
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- W2034451538 title "DNA Sequences Essential for Transcription of Human Phospholipid Transfer Protein Gene in HepG2 Cells" @default.
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- W2034451538 doi "https://doi.org/10.1006/bbrc.1997.6330" @default.
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