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- W2034466071 abstract "Of the two synthetic polyanions poly-(styrene sulfonate) (PSS) and poly-(vinyl sulfate) (PVS), the latter induces sharper metachromasia in the cationic dye crystal violet (CV); DNA, however, fails to induce metachromasia in the dye. Contrary to speculation, DNA binds CV, though a nonintercalating dye, rather strongly and holds a portion of the dye even in the presence of PSS or PVS, which induces strong metachromasia. Induced circular dichroism (ICD) in CV by DNA shows that DNA binds CV, and partial reduction of ellipticity of DNA-CV by PVS shows that CV distributes itself between the two polyanions. DNA is shown to be the winner in the competitive binding of CV even with the strong polyanion heparin (Hep) as the competitor. This has been interpreted as being due to the binding of CV to DNA, primarily by ionic attraction stabilized further by nonionic forces like H-bonding in the grooves of DNA. Quantitative estimations of the association constants of DNA-CV and PVS-CV by Scatchard plot come to 0.74 × 106 and 1.28 × 106 mol−1, respectively." @default.
- W2034466071 created "2016-06-24" @default.
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- W2034466071 date "1994-01-01" @default.
- W2034466071 modified "2023-09-26" @default.
- W2034466071 title "Circular dichroic and spectrophotometric probes of the competitive binding of crystal violet between DNA and other anionic polymers" @default.
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- W2034466071 doi "https://doi.org/10.1016/0584-8539(94)80119-3" @default.
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