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- W2034479066 abstract "Abstract The development of an indirect competitive enzyme immunoassay for the sulfonylurea herbicide metsulfuron-methyl (MSM) is described. In contrast to traditional antibody generation in mammals, this extremely sensitive method is based on chicken egg yolk antibodies (IgY). They were raised in laying hens using an MSM-derivative-BSA hapten as immunogen. With a 1:10000 dilution of the antibody solution and a coating antigen (MSM-derivative-KLH) concentration of 10 μg L−1 the IC50 value achieved for the target analyte was 0.4 μg L−1. The least detectable dose was established at 13 ng L−1. Cross-reactivity was tested with 5 structurally related compounds, where only sulfometuron showed a significant binding. The ELISA was tested with spiked tap and surface water samples. This paper, for the first time, demonstrates the production of high-affinity IgY antibodies for a herbicide compound." @default.
- W2034479066 created "2016-06-24" @default.
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- W2034479066 date "2000-11-01" @default.
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- W2034479066 title "Development of an Enzyme Immunoassay for the Determination of the Herbicide Metsulfuron-Methyl Based on Chicken Egg Yolk Antibodies" @default.
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- W2034479066 doi "https://doi.org/10.1080/03067310008041347" @default.
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