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• W2034502026 abstract "A method using semi-automatic microextraction by packed sorbent (eVol®-MEPS) and hydrophilic interaction chromatography–ultra-high-performance liquid chromatography–tandem mass spectrometry (HILIC–UHPLC–MS/MS) was described for the simultaneous determination of carnitine and acylcarnitines in human urine. The optimal conditions of MEPS extraction were obtained using C2 of M1 (C8 + SCX) phase as a sorbent. Chromatographic separation of the analytes was achieved within 2.5 min on Acquity UPLC BEH HILIC column using a gradient elution program with water containing 5 mM ammonium acetate and acetonitrile as the mobile phase. The detection was performed on a triple-quadrupole tandem mass spectrometer in a positive ion mode via electrospray ionization (ESI). The linearity of the calibration curves for all compounds was found over a range from 0.1 ng/mL to 500 ng/mL. The method afforded satisfactory results in terms of sensitivity, specificity, precision, accuracy, recovery as well as stability of the analyte under various conditions. The method was used successfully for determination of carnitine and acylcarnitines in human urine." @default.
• W2034502026 created "2016-06-24" @default.
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• W2034502026 date "2015-05-01" @default.
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• W2034502026 title "Determination of carnitine and acylcarnitines in human urine by means of microextraction in packed sorbent and hydrophilic interaction chromatography–ultra-high-performance liquid chromatography–tandem mass spectrometry" @default.
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• W2034502026 doi "https://doi.org/10.1016/j.jpba.2015.02.044" @default.
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