Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034603471> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2034603471 abstract "Abstract Trehalose 6-phosphate synthase was solubilized from young sorocarps of the cellular slime mold, Dictyostelium discoideum, by a freeze-thaw cycle and was subsequently purified about 160-fold using streptomycin sulfate precipitation, (NH4)2SO4 fractionation, DEAE-cellulose chromatography, heat treatment in the presence of heparin, and molecular sieve chromatography on columns of Bio-Gel A-1.5m. The purified enzyme was maximally active at pH 6.5, showed an absolute specificity for glucose 6-phosphate as glucosyl acceptor and a relative specificity for the glucosyl donor in the order: UDP-glucose, GDP-glucose, and ADP-glucose. Although heparin and chondroitin sulfate activated the synthase, the order of glucosyl donor specificity was not affected. Other activators of trehalose 6-phosphate synthase were KCL, Mg2+, and EDTA, while detergents had little effect. Although synthase activity was reduced 60 to 80% upon the omission of Mg2+ from the assay mixture, an absolute dependency for Mg2+ could not be demonstrated. Evaluation of the apparent Km values for partially purified synthase preparations demonstrated that for each of the synthase substrates, the Line weaver-Burk plots displayed complex bimodal kinetics. Estimation of the Michaelis constants after extrapolation of the straight line portions of these plots yielded values of (a) 0.2 and 3.2 m m glucose 6-phosphate and (b) 0.5 and 2.2 m m UDP-glucose. Comparison of the latter parameters with the cellular levels of UDP-glucose and glucose 6-phosphate in Dictyostelium suggests that if the observed bimodal kinetics are the consequence of multiple kinetically distinct forms of the synthase, the activation of trehalose synthesis during slime mold culmination could provide a rationale for the presence of these isozymes." @default.
- W2034603471 created "2016-06-24" @default.
- W2034603471 creator A5016168114 @default.
- W2034603471 date "1979-08-01" @default.
- W2034603471 modified "2023-09-26" @default.
- W2034603471 title "Trehalose 6-phosphate synthase from Dictyostelium discoideum: Partial purification and characterization of the enzyme from young sorocarps" @default.
- W2034603471 cites W1523878873 @default.
- W2034603471 cites W1533313516 @default.
- W2034603471 cites W1567723438 @default.
- W2034603471 cites W1573838248 @default.
- W2034603471 cites W1592268302 @default.
- W2034603471 cites W1622360991 @default.
- W2034603471 cites W1775749144 @default.
- W2034603471 cites W1886189877 @default.
- W2034603471 cites W19794046 @default.
- W2034603471 cites W1982942510 @default.
- W2034603471 cites W1999720921 @default.
- W2034603471 cites W2003953466 @default.
- W2034603471 cites W2016098487 @default.
- W2034603471 cites W2043471031 @default.
- W2034603471 cites W2057193621 @default.
- W2034603471 cites W2058093765 @default.
- W2034603471 cites W2069401794 @default.
- W2034603471 cites W2069556249 @default.
- W2034603471 cites W2087452949 @default.
- W2034603471 cites W2138158216 @default.
- W2034603471 cites W2143530346 @default.
- W2034603471 cites W346714302 @default.
- W2034603471 doi "https://doi.org/10.1016/0003-9861(79)90558-7" @default.
- W2034603471 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/159657" @default.
- W2034603471 hasPublicationYear "1979" @default.
- W2034603471 type Work @default.
- W2034603471 sameAs 2034603471 @default.
- W2034603471 citedByCount "26" @default.
- W2034603471 countsByYear W20346034712012 @default.
- W2034603471 countsByYear W20346034712018 @default.
- W2034603471 countsByYear W20346034712019 @default.
- W2034603471 crossrefType "journal-article" @default.
- W2034603471 hasAuthorship W2034603471A5016168114 @default.
- W2034603471 hasConcept C104317684 @default.
- W2034603471 hasConcept C112243037 @default.
- W2034603471 hasConcept C181199279 @default.
- W2034603471 hasConcept C185592680 @default.
- W2034603471 hasConcept C193363816 @default.
- W2034603471 hasConcept C2775969001 @default.
- W2034603471 hasConcept C2778452849 @default.
- W2034603471 hasConcept C2780535078 @default.
- W2034603471 hasConcept C43617362 @default.
- W2034603471 hasConcept C55493867 @default.
- W2034603471 hasConceptScore W2034603471C104317684 @default.
- W2034603471 hasConceptScore W2034603471C112243037 @default.
- W2034603471 hasConceptScore W2034603471C181199279 @default.
- W2034603471 hasConceptScore W2034603471C185592680 @default.
- W2034603471 hasConceptScore W2034603471C193363816 @default.
- W2034603471 hasConceptScore W2034603471C2775969001 @default.
- W2034603471 hasConceptScore W2034603471C2778452849 @default.
- W2034603471 hasConceptScore W2034603471C2780535078 @default.
- W2034603471 hasConceptScore W2034603471C43617362 @default.
- W2034603471 hasConceptScore W2034603471C55493867 @default.
- W2034603471 hasLocation W20346034711 @default.
- W2034603471 hasLocation W20346034712 @default.
- W2034603471 hasOpenAccess W2034603471 @default.
- W2034603471 hasPrimaryLocation W20346034711 @default.
- W2034603471 hasRelatedWork W1968331688 @default.
- W2034603471 hasRelatedWork W1988695982 @default.
- W2034603471 hasRelatedWork W1991996737 @default.
- W2034603471 hasRelatedWork W2000789859 @default.
- W2034603471 hasRelatedWork W2033545996 @default.
- W2034603471 hasRelatedWork W2034603471 @default.
- W2034603471 hasRelatedWork W2047347058 @default.
- W2034603471 hasRelatedWork W2101421032 @default.
- W2034603471 hasRelatedWork W2169052801 @default.
- W2034603471 hasRelatedWork W2187425755 @default.
- W2034603471 isParatext "false" @default.
- W2034603471 isRetracted "false" @default.
- W2034603471 magId "2034603471" @default.
- W2034603471 workType "article" @default.