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• W2034606071 endingPage "469" @default.
• W2034606071 startingPage "461" @default.
• W2034606071 abstract "This paper presents novel methods to produce arrays of lipid bilayers and liposomes on patterned polyelectrolyte multilayers. We created the arrays by exposing patterns of poly(dimethyldiallylammonium chloride) (PDAC), polyethylene glycol (m-dPEG) acid, and poly(allylamine hydrochloride) (PAH) on polyelectrolyte multilayers (PEMs) to liposomes of various compositions. The resulting interfaces were characterized by total internal reflection fluorescence microscopy (TIRFM), fluorescence recovery after pattern photobleaching (FRAPP), quartz crystal microbalance (QCM), and fluorescence microscopy. Liposomes composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phosphate (monosodium salt) (DOPA) were found to preferentially adsorb on PDAC and PAH surfaces. On the other hand, liposome adsorption on sulfonated poly(styrene) (SPS) surfaces was minimal, due to electrostatic repulsion between the negatively charged liposomes and the SPS-coated surface. Surfaces coated with m-dPEG acid were also found to resist liposome adsorption. We exploited these results to create arrays of lipid bilayers by exposing PDAC, PAH and m-dPEG patterned substrates to DOPA/DOPC vesicles of various compositions. The patterned substrates were created by stamping PDAC (or PAH) on SPS-topped multilayers, and m-dPEG acid on PDAC-topped multilayers, respectively. This technique can be used to produce functional biomimetic interfaces for potential applications in biosensors and biocatalysis, for creating arrays that could be used for high-throughput screening of compounds that interact with cell membranes, and for probing, and possibly controlling, interactions between living cells and synthetic membranes." @default.
• W2034606071 created "2016-06-24" @default.
• W2034606071 creator A5003879275 @default.
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• W2034606071 date "2006-09-01" @default.
• W2034606071 modified "2023-10-16" @default.
• W2034606071 title "Arrays of lipid bilayers and liposomes on patterned polyelectrolyte templates" @default.
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• W2034606071 doi "https://doi.org/10.1016/j.jcis.2006.05.048" @default.
• W2034606071 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16790245" @default.
• W2034606071 hasPublicationYear "2006" @default.
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