Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034606397> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2034606397 endingPage "6519" @default.
- W2034606397 startingPage "6507" @default.
- W2034606397 abstract "A group I intron precursor and ribozyme were cloned from the large subunit rRNA of the human pathogen Candida albicans. Both the precursor and ribozyme are functional as determined from in vitro assays. Comparisons of dissociation constants for oligonucleotide binding to the ribozyme and to a hexanucleotide mimic of its internal guide sequence lead to a model for recognition of the 5‘ exon substrate by this intron. In particular, tertiary contacts with the P1 helix that help align the splice site include three 2‘-hydroxyl groups, a G·U pair that occurs at the intron's splice junction, and a G·A pair. The free energy contribution that each interaction contributes to tertiary binding is determined. When the G·A pair is replaced with a G-C pair, tertiary interactions to 5‘ exon mimic 2‘-hydroxyl groups are significantly weakened. When the G·A pair is replaced with a G·U pair, tertiary interactions are retained and binding is 10-fold tighter. These results expand our knowledge of substrate recognition by group I introns, and also provide a basis for rational design of oligonucleotide-based therapeutics for targeting group I introns by binding enhancement by tertiary interactions and suicide inhibition strategies." @default.
- W2034606397 created "2016-06-24" @default.
- W2034606397 creator A5013801926 @default.
- W2034606397 creator A5017275370 @default.
- W2034606397 creator A5018835557 @default.
- W2034606397 date "2001-05-01" @default.
- W2034606397 modified "2023-09-27" @default.
- W2034606397 title "Recognition Elements for 5‘ Exon Substrate Binding to the <i>Candida albicans</i> Group I Intron" @default.
- W2034606397 cites W2002269154 @default.
- W2034606397 cites W2009818318 @default.
- W2034606397 cites W2016791718 @default.
- W2034606397 cites W2065837503 @default.
- W2034606397 doi "https://doi.org/10.1021/bi002008r" @default.
- W2034606397 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11371214" @default.
- W2034606397 hasPublicationYear "2001" @default.
- W2034606397 type Work @default.
- W2034606397 sameAs 2034606397 @default.
- W2034606397 citedByCount "20" @default.
- W2034606397 countsByYear W20346063972012 @default.
- W2034606397 countsByYear W20346063972014 @default.
- W2034606397 countsByYear W20346063972016 @default.
- W2034606397 crossrefType "journal-article" @default.
- W2034606397 hasAuthorship W2034606397A5013801926 @default.
- W2034606397 hasAuthorship W2034606397A5017275370 @default.
- W2034606397 hasAuthorship W2034606397A5018835557 @default.
- W2034606397 hasConcept C104317684 @default.
- W2034606397 hasConcept C107824862 @default.
- W2034606397 hasConcept C118346598 @default.
- W2034606397 hasConcept C129312508 @default.
- W2034606397 hasConcept C185592680 @default.
- W2034606397 hasConcept C2780917455 @default.
- W2034606397 hasConcept C36823959 @default.
- W2034606397 hasConcept C54355233 @default.
- W2034606397 hasConcept C54458228 @default.
- W2034606397 hasConcept C64962909 @default.
- W2034606397 hasConcept C67705224 @default.
- W2034606397 hasConcept C71240020 @default.
- W2034606397 hasConcept C76346623 @default.
- W2034606397 hasConcept C86803240 @default.
- W2034606397 hasConcept C94671646 @default.
- W2034606397 hasConceptScore W2034606397C104317684 @default.
- W2034606397 hasConceptScore W2034606397C107824862 @default.
- W2034606397 hasConceptScore W2034606397C118346598 @default.
- W2034606397 hasConceptScore W2034606397C129312508 @default.
- W2034606397 hasConceptScore W2034606397C185592680 @default.
- W2034606397 hasConceptScore W2034606397C2780917455 @default.
- W2034606397 hasConceptScore W2034606397C36823959 @default.
- W2034606397 hasConceptScore W2034606397C54355233 @default.
- W2034606397 hasConceptScore W2034606397C54458228 @default.
- W2034606397 hasConceptScore W2034606397C64962909 @default.
- W2034606397 hasConceptScore W2034606397C67705224 @default.
- W2034606397 hasConceptScore W2034606397C71240020 @default.
- W2034606397 hasConceptScore W2034606397C76346623 @default.
- W2034606397 hasConceptScore W2034606397C86803240 @default.
- W2034606397 hasConceptScore W2034606397C94671646 @default.
- W2034606397 hasIssue "21" @default.
- W2034606397 hasLocation W20346063971 @default.
- W2034606397 hasLocation W20346063972 @default.
- W2034606397 hasOpenAccess W2034606397 @default.
- W2034606397 hasPrimaryLocation W20346063971 @default.
- W2034606397 hasRelatedWork W1554164208 @default.
- W2034606397 hasRelatedWork W1965174422 @default.
- W2034606397 hasRelatedWork W2008165719 @default.
- W2034606397 hasRelatedWork W2034715925 @default.
- W2034606397 hasRelatedWork W2046126070 @default.
- W2034606397 hasRelatedWork W2050188885 @default.
- W2034606397 hasRelatedWork W2100783529 @default.
- W2034606397 hasRelatedWork W2102460294 @default.
- W2034606397 hasRelatedWork W3214413750 @default.
- W2034606397 hasRelatedWork W83247859 @default.
- W2034606397 hasVolume "40" @default.
- W2034606397 isParatext "false" @default.
- W2034606397 isRetracted "false" @default.
- W2034606397 magId "2034606397" @default.
- W2034606397 workType "article" @default.