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- W2034608210 abstract "Background: Photodynamic therapy utilizes a photosensitizer, which upon excitation, emits energy that could be transduced by surrounding molecular oxygen to produce radical oxygen species. Interaction between the singlet oxygen species and viruses may have a virucidal effect. In this study, the potential of using a novel photodynamic system to inactivate enveloped virus was investigated. Methods: The photosensitizer used was zincphthalocyanine (ZnPC), which was absorbed onto a novel two-photon nanotransducer (NaYF4) that is activated by near infrared light (NIR). Dengue 2 virus was mixed with different concentrations of nanoparticles and exposed to a NIR light source at varying distances (5 cm and 10 cm) and durations (5 minutes and 10 minutes). The amount of surviving viruses was analyzed using plaque assay and compared with untreated virus samples. Further photoinactivation study is being done on dengue 2 virus-infected HepG2 cells using nanoparticles that were conjugated with antibodies specific for the dengue virus envelope protein. The infected cells were incubated with the antibodyconjugated nanoparticles (4 to 110g/mL) and subjected to NIR illumination (10 cm for 5 minutes). Results: The level of virus inactivation increased with higher nanoparticle concentrations and at shorter distance and longer illumination from the NIR source. At all conditions, virus inactivation was complete when 2.2 mg/mL nanoparticles were used and at 44g/mL, virus titer was reduced by more than 50%. Conjugation with antibody may enhance the localization of the photo-inactivation site to targeted viruses or to virus-infected cells only, as shown by electron and laser scanning confocal microscopy. In addition, cell viability (MTT) assay showed that the photobleaching effect by NIR light on HepG2 cells was only minimal. Conclusion: Given its effectiveness in eradicating virus activity and its non-cytotoxic effect on cells, photodynamic therapy using NIR light could be a feasible novel technology with important implications in treating virus-infected blood or cellular samples." @default.
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- W2034608210 date "2008-12-01" @default.
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- W2034608210 title "Susceptibility to Oseltamivir and Amantadine of Human Influenza Viruses Circulating in Portugal" @default.
- W2034608210 doi "https://doi.org/10.1016/j.ijid.2008.05.815" @default.
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