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- W2034608439 abstract "The molecular cloning and characterization of PipsRR1, a type-A response regulator in Pinus pinaster, is reported here. Type-A response regulators mediate downstream responses to cytokinin and act as negative feedback regulators of the signal transduction pathway. Some type-A response regulators in Arabidopsis have been related to de novo meristem formation. However, little information exists in Pinus spp. The PipsRR1 gene contains 5 exons, as do all type-A response regulators in Arabidopsis, and the deduced protein contains a receiver domain with the conserved DDK residues and a short C terminal extension. Expression analysis showed that the PipsRR1 gene is differentially expressed during the first phases of adventitious caulogenesis induced by benzyladenine in P. pinaster cotyledons, suggesting that PipsRR1 plays a role in caulogenesis in conifers. Additionally, a binary vector carrying the PipsRR1 promoter driving GFP:GUS expression was constructed to analyze the promoter activity in P. pinaster somatic embryos. The results of genetic transformation showed GUS activity during somatic embryo mass proliferation and embryo maturation." @default.
- W2034608439 created "2016-06-24" @default.
- W2034608439 creator A5073783569 @default.
- W2034608439 creator A5075819993 @default.
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- W2034608439 date "2012-12-01" @default.
- W2034608439 modified "2023-10-14" @default.
- W2034608439 title "Characterization of a type-A response regulator differentially expressed during adventitious caulogenesis in Pinus pinaster" @default.
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- W2034608439 doi "https://doi.org/10.1016/j.jplph.2012.07.014" @default.
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