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- W2034608454 endingPage "774" @default.
- W2034608454 startingPage "751" @default.
- W2034608454 abstract "Abstract Among the different disciplines covered by mass spectrometry, measurement of 13 C/ 12 C isotopic ratio crosses a large section of disciplines from a tool revealing the origin of compounds to more recent approaches such as metabolomics and proteomics. Isotope ratio mass spectrometry (IRMS) and molecular mass spectrometry (MS) are the two most mature techniques for 13 C isotopic analysis of compounds, respectively, for high and low‐isotopic precision. For the sample introduction, the coupling of gas chromatography (GC) to either IRMS or MS is state of the art technique for targeted isotopic analysis of volatile analytes. However, liquid chromatography (LC) also needs to be considered as a tool for the sample introduction into IRMS or MS for 13 C isotopic analyses of non‐volatile analytes at natural abundance as well as for 13 C‐labeled compounds. This review presents the past and the current processes used to perform 13 C isotopic analysis in combination with LC. It gives particular attention to the combination of LC with IRMS which started in the 1990's with the moving wire transport, then subsequently moved to the chemical reaction interface (CRI) and was made commercially available in 2004 with the wet chemical oxidation interface (LC‐IRMS). The LC‐IRMS method development is also discussed in this review, including the possible approaches for increasing selectivity and efficiency, for example, using a 100% aqueous mobile phase for the LC separation. In addition, applications for measuring 13 C isotopic enrichments using atmospheric pressure LC‐MS instruments with a quadrupole, a time‐of‐flight, and an ion trap analyzer are also discussed as well as a LC‐ICPMS using a prototype instrument with two quadrupoles. © 2007 Wiley Periodicals, Inc., Mass Spec Rev 26:751–774, 2007" @default.
- W2034608454 created "2016-06-24" @default.
- W2034608454 creator A5029276480 @default.
- W2034608454 creator A5082553278 @default.
- W2034608454 creator A5083416984 @default.
- W2034608454 date "2007-09-12" @default.
- W2034608454 modified "2023-10-12" @default.
- W2034608454 title "Liquid chromatography combined with mass spectrometry for13C isotopic analysis in life science research" @default.
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