FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034608470> ?p ?o ?g. }

• W2034608470 endingPage "3349" @default.
• W2034608470 startingPage "3338" @default.
• W2034608470 abstract "The human immunodeficiency virus type 1 transmembrane glycoprotein (TM) is efficiently endocytosed in a clathrin-dependent manner. Internalization is mediated by a tyrosine-containing motif within the cytoplasmic domain, and replacement of the cytoplasmic tyrosine by cysteine or phenylalanine increased expression of mutant glycoprotein on the surface of transfected cells by as much as 2.5-fold. Because interactions between the cytoplasmic domain of Env and the matrix protein (MA) have been suggested to mediate incorporation of Env in virus particles, we examined whether perturbation of endocytosis would alter incorporation. Proviruses were constructed to contain the wild-type or mutant Env in conjunction with point mutations in MA that had previously been shown to block Env incorporation. These constructs were used to evaluate the effect of glycoprotein endocytosis on incorporation into virus particles and to test the necessity for a specific interaction between Env and MA to mediate incorporation. Viruses produced from transfected 293T cells were used to infect various cell lines, including MAGI, H9, and CEMx174. Viruses encoding both a disrupted endocytosis motif signal and mutations within MA were significantly more infectious in MAGI cells than their counterparts encoding a mutant MA and wild-type Env. This complementation of infectivity for the MA incorporation mutant viruses was not due to increased glycoprotein incorporation into particles but instead reflected an enhanced fusogenicity of the mutated Env proteins. Our findings further support the concept that a specific interaction between the long cytoplasmic domain of TM and MA is required for efficient incorporation of Env into assembling virions. Alteration of the endocytosis signal of Env, and the resulting increase in cell surface glycoprotein, has no effect on incorporation despite demonstrable effects on fusion, virus entry, and infectivity." @default.
• W2034608470 created "2016-06-24" @default.
• W2034608470 creator A5007884563 @default.
• W2034608470 creator A5008968738 @default.
• W2034608470 creator A5012871042 @default.
• W2034608470 creator A5027084909 @default.
• W2034608470 creator A5035881745 @default.
• W2034608470 creator A5052560107 @default.
• W2034608470 creator A5072633844 @default.
• W2034608470 date "2002-04-01" @default.
• W2034608470 modified "2023-10-17" @default.
• W2034608470 title "Mutation of the Dominant Endocytosis Motif in Human Immunodeficiency Virus Type 1 gp41 Can Complement Matrix Mutations without Increasing Env Incorporation" @default.
• W2034608470 cites W1480555318 @default.
• W2034608470 cites W1487627529 @default.
• W2034608470 cites W1511364129 @default.
• W2034608470 cites W1523294305 @default.
• W2034608470 cites W1526795140 @default.
• W2034608470 cites W1531431198 @default.
• W2034608470 cites W1567879434 @default.
• W2034608470 cites W1596881379 @default.
• W2034608470 cites W1601854877 @default.
• W2034608470 cites W1611298747 @default.
• W2034608470 cites W1627636575 @default.
• W2034608470 cites W1632340368 @default.
• W2034608470 cites W1632486555 @default.
• W2034608470 cites W1659840293 @default.
• W2034608470 cites W170446279 @default.
• W2034608470 cites W1715327714 @default.
• W2034608470 cites W1760183277 @default.
• W2034608470 cites W1762245052 @default.
• W2034608470 cites W1802173282 @default.
• W2034608470 cites W1837543172 @default.
• W2034608470 cites W1880482445 @default.
• W2034608470 cites W1884404343 @default.
• W2034608470 cites W1896763970 @default.
• W2034608470 cites W1959432277 @default.
• W2034608470 cites W1987672004 @default.
• W2034608470 cites W2008457983 @default.
• W2034608470 cites W2009205890 @default.
• W2034608470 cites W2009599622 @default.
• W2034608470 cites W2012956482 @default.
• W2034608470 cites W2015943185 @default.
• W2034608470 cites W2019469658 @default.
• W2034608470 cites W2019908099 @default.
• W2034608470 cites W2021080732 @default.
• W2034608470 cites W2024764884 @default.
• W2034608470 cites W2032232188 @default.
• W2034608470 cites W2034988569 @default.
• W2034608470 cites W2043555842 @default.
• W2034608470 cites W2044625086 @default.
• W2034608470 cites W2050960932 @default.
• W2034608470 cites W2052678796 @default.
• W2034608470 cites W2058032164 @default.
• W2034608470 cites W2067691363 @default.
• W2034608470 cites W2079420925 @default.
• W2034608470 cites W2082091164 @default.
• W2034608470 cites W2082861222 @default.
• W2034608470 cites W2085355019 @default.
• W2034608470 cites W2092072942 @default.
• W2034608470 cites W2095294646 @default.
• W2034608470 cites W2095606352 @default.
• W2034608470 cites W2099491605 @default.
• W2034608470 cites W2101685255 @default.
• W2034608470 cites W2104148336 @default.
• W2034608470 cites W2108623121 @default.
• W2034608470 cites W2112786488 @default.
• W2034608470 cites W2114653876 @default.
• W2034608470 cites W2116795517 @default.
• W2034608470 cites W2117788130 @default.
• W2034608470 cites W2123852904 @default.
• W2034608470 cites W2131134035 @default.
• W2034608470 cites W2131803903 @default.
• W2034608470 cites W213218024 @default.
• W2034608470 cites W2137401529 @default.
• W2034608470 cites W2137876144 @default.
• W2034608470 cites W2138263779 @default.
• W2034608470 cites W2139675968 @default.
• W2034608470 cites W2141162592 @default.
• W2034608470 cites W2141825526 @default.
• W2034608470 cites W2142204958 @default.
• W2034608470 cites W2146285125 @default.
• W2034608470 cites W2148827252 @default.
• W2034608470 cites W2153927912 @default.
• W2034608470 cites W2156010727 @default.
• W2034608470 cites W2162183496 @default.
• W2034608470 cites W2163519490 @default.
• W2034608470 cites W2171541353 @default.
• W2034608470 cites W2178812731 @default.
• W2034608470 cites W311244383 @default.
• W2034608470 doi "https://doi.org/10.1128/jvi.76.7.3338-3349.2002" @default.
• W2034608470 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/136014" @default.
• W2034608470 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11884559" @default.
• W2034608470 hasPublicationYear "2002" @default.
• W2034608470 type Work @default.
• W2034608470 sameAs 2034608470 @default.
• W2034608470 citedByCount "49" @default.
• W2034608470 countsByYear W20346084702012 @default.
• W2034608470 countsByYear W20346084702013 @default.

• next