FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034621544> ?p ?o ?g. }

• W2034621544 endingPage "6345" @default.
• W2034621544 startingPage "6337" @default.
• W2034621544 abstract "Component-resolved diagnostic (CRD) of cow’s milk allergy has been performed using immunoaffinity capillary electrophoresis (IACE) coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). First, total IgE quantification in the blood serum of a milk allergic patient by the IACE-UV technique was developed using magnetic beads (MBs) coated with antihuman IgE antibodies (Abs) to perform the general allergy diagnosis. Then, the immunocomplex of antihuman IgE Abs with the patient IgE Abs, obtained during the total IgE analysis, was chemically cross-linked on the MBs surface. Prepared immunosupport was used for the binding of individual milk allergens to identify the proteins triggering the allergy by IACE with UV and MALDI MS detection. Then, allergy CRD was also performed directly with milk fractions. Bovine serum albumin, lactoferrin, and α-casein (S1 and S2 forms, as was revealed by MALDI MS) were found to bind with the extracted IgE Abs, indicating that the chosen patient is allergic to these proteins. The results were confirmed by performing classical enzyme-linked immunosorbent assay of total and specific IgE Abs. The present IACE-UV/MALDI MS method required only 2 μL of blood serum and allowed the performance of the total IgE quantification and CRD of the food allergy not only with the purified allergen molecules but also directly with the food extract. Such an approach opens the possibility for direct identification of allergens molecular mass and structure, discovery of unusual allergens, which could be useful for precise personalized allergy diagnostic, allergens epitope mapping, and cross-reactivity studies." @default.
• W2034621544 created "2016-06-24" @default.
• W2034621544 creator A5010061178 @default.
• W2034621544 creator A5068967401 @default.
• W2034621544 date "2014-06-20" @default.
• W2034621544 modified "2023-10-03" @default.
• W2034621544 title "Component-Resolved Diagnostic of Cow’s Milk Allergy by Immunoaffinity Capillary Electrophoresis–Matrix Assisted Laser Desorption/Ionization Mass Spectrometry" @default.
• W2034621544 cites W1896406023 @default.
• W2034621544 cites W1973545017 @default.
• W2034621544 cites W1983414328 @default.
• W2034621544 cites W1991273392 @default.
• W2034621544 cites W1995913414 @default.
• W2034621544 cites W1997924568 @default.
• W2034621544 cites W2007455368 @default.
• W2034621544 cites W2009160282 @default.
• W2034621544 cites W2015547194 @default.
• W2034621544 cites W2029736701 @default.
• W2034621544 cites W2040910365 @default.
• W2034621544 cites W2047583003 @default.
• W2034621544 cites W2048496140 @default.
• W2034621544 cites W2048817142 @default.
• W2034621544 cites W2059134849 @default.
• W2034621544 cites W2063659982 @default.
• W2034621544 cites W2070940305 @default.
• W2034621544 cites W2077564873 @default.
• W2034621544 cites W2078716447 @default.
• W2034621544 cites W2092664207 @default.
• W2034621544 cites W2096178587 @default.
• W2034621544 cites W2105568704 @default.
• W2034621544 cites W2116311099 @default.
• W2034621544 cites W2117688259 @default.
• W2034621544 cites W2131923430 @default.
• W2034621544 cites W2137820896 @default.
• W2034621544 cites W2152865924 @default.
• W2034621544 cites W2159412770 @default.
• W2034621544 cites W2169771014 @default.
• W2034621544 cites W2172116519 @default.
• W2034621544 cites W2314461424 @default.
• W2034621544 cites W2332256334 @default.
• W2034621544 doi "https://doi.org/10.1021/ac500525n" @default.
• W2034621544 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24915289" @default.
• W2034621544 hasPublicationYear "2014" @default.
• W2034621544 type Work @default.
• W2034621544 sameAs 2034621544 @default.
• W2034621544 citedByCount "30" @default.
• W2034621544 countsByYear W20346215442015 @default.
• W2034621544 countsByYear W20346215442016 @default.
• W2034621544 countsByYear W20346215442017 @default.
• W2034621544 countsByYear W20346215442018 @default.
• W2034621544 countsByYear W20346215442019 @default.
• W2034621544 countsByYear W20346215442020 @default.
• W2034621544 countsByYear W20346215442021 @default.
• W2034621544 countsByYear W20346215442022 @default.
• W2034621544 countsByYear W20346215442023 @default.
• W2034621544 crossrefType "journal-article" @default.
• W2034621544 hasAuthorship W2034621544A5010061178 @default.
• W2034621544 hasAuthorship W2034621544A5068967401 @default.
• W2034621544 hasBestOaLocation W20346215442 @default.
• W2034621544 hasConcept C141105273 @default.
• W2034621544 hasConcept C150394285 @default.
• W2034621544 hasConcept C159654299 @default.
• W2034621544 hasConcept C162356407 @default.
• W2034621544 hasConcept C162711632 @default.
• W2034621544 hasConcept C178790620 @default.
• W2034621544 hasConcept C185592680 @default.
• W2034621544 hasConcept C203014093 @default.
• W2034621544 hasConcept C207480886 @default.
• W2034621544 hasConcept C2776027960 @default.
• W2034621544 hasConcept C2779142602 @default.
• W2034621544 hasConcept C2780510475 @default.
• W2034621544 hasConcept C2781372412 @default.
• W2034621544 hasConcept C2900643 @default.
• W2034621544 hasConcept C3020064264 @default.
• W2034621544 hasConcept C43617362 @default.
• W2034621544 hasConcept C75280812 @default.
• W2034621544 hasConcept C86803240 @default.
• W2034621544 hasConceptScore W2034621544C141105273 @default.
• W2034621544 hasConceptScore W2034621544C150394285 @default.
• W2034621544 hasConceptScore W2034621544C159654299 @default.
• W2034621544 hasConceptScore W2034621544C162356407 @default.
• W2034621544 hasConceptScore W2034621544C162711632 @default.
• W2034621544 hasConceptScore W2034621544C178790620 @default.
• W2034621544 hasConceptScore W2034621544C185592680 @default.
• W2034621544 hasConceptScore W2034621544C203014093 @default.
• W2034621544 hasConceptScore W2034621544C207480886 @default.
• W2034621544 hasConceptScore W2034621544C2776027960 @default.
• W2034621544 hasConceptScore W2034621544C2779142602 @default.
• W2034621544 hasConceptScore W2034621544C2780510475 @default.
• W2034621544 hasConceptScore W2034621544C2781372412 @default.
• W2034621544 hasConceptScore W2034621544C2900643 @default.
• W2034621544 hasConceptScore W2034621544C3020064264 @default.
• W2034621544 hasConceptScore W2034621544C43617362 @default.
• W2034621544 hasConceptScore W2034621544C75280812 @default.
• W2034621544 hasConceptScore W2034621544C86803240 @default.
• W2034621544 hasIssue "13" @default.
• W2034621544 hasLocation W20346215441 @default.
• W2034621544 hasLocation W20346215442 @default.
• W2034621544 hasLocation W20346215443 @default.

• next