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- W2034626250 abstract "Inhibin is a dimeric peptide hormone produced in ovarian granulosa cells that suppresses FSH synthesis and secretion in the pituitary. Expression of inhibin α- and β-subunit genes in the rodent ovary is positively regulated by FSH and negatively regulated after the preovulatory LH surge. We have investigated the role of the transcription factor CCAAT/enhancer-binding protein-β (C/EBPβ) in repressing the inhibin α-subunit gene. C/EBPβ knockout mice fail to appropriately down-regulate inhibin α-subunit mRNA levels after treatment with human chorionic gonadotropin, indicating that C/EBPβ may function to repress inhibin gene expression. The expression and regulation of C/EBPβ were examined in rodent ovary, and these studies show that C/EBPβ is expressed in ovary and granulosa cells and is induced in response to human chorionic gonadotropin. Transient cotransfections with an inhibin promoter-luciferase reporter in a mouse granulosa cell line, GRMO2 cells, show that C/EBPβ is capable of repressing both basal and forskolin-stimulated inhibin gene promoter activities. An upstream binding site for C/EBPβ in the inhibin α-subunit promoter was identified by electrophoretic mobility shift assays, which, when mutated, results in elevated inhibin promoter activity. However, C/EBPβ also represses shorter promoter constructs lacking this site, and this component of repression is dependent on the more proximal promoter cAMP response element (CRE). Electrophoretic mobility shift assays show that C/EBPβ effectively competes with CRE-binding protein for binding to this atypical CRE. Thus, there are two distinct mechanisms by which C/EBPβ represses inhibin α-subunit gene expression in ovarian granulosa cells." @default.
- W2034626250 created "2016-06-24" @default.
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- W2034626250 creator A5047897066 @default.
- W2034626250 creator A5055696858 @default.
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- W2034626250 date "2005-04-01" @default.
- W2034626250 modified "2023-10-04" @default.
- W2034626250 title "Repression of the Inhibin α-Subunit Gene by the Transcription Factor CCAAT/Enhancer-Binding Protein-β" @default.
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- W2034626250 cites W1499501743 @default.
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- W2034626250 cites W1534163224 @default.
- W2034626250 cites W161652132 @default.
- W2034626250 cites W1676121678 @default.
- W2034626250 cites W1751435293 @default.
- W2034626250 cites W1786284230 @default.
- W2034626250 cites W1807295484 @default.
- W2034626250 cites W1933156589 @default.
- W2034626250 cites W1943421922 @default.
- W2034626250 cites W1971290197 @default.
- W2034626250 cites W1973867548 @default.
- W2034626250 cites W1974308772 @default.
- W2034626250 cites W1986184556 @default.
- W2034626250 cites W1989481014 @default.
- W2034626250 cites W1992308140 @default.
- W2034626250 cites W1993243592 @default.
- W2034626250 cites W1995322263 @default.
- W2034626250 cites W1996339709 @default.
- W2034626250 cites W1998448534 @default.
- W2034626250 cites W2000024512 @default.
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- W2034626250 cites W2003330976 @default.
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- W2034626250 cites W2007080316 @default.
- W2034626250 cites W2007227919 @default.
- W2034626250 cites W2020189783 @default.
- W2034626250 cites W2020649739 @default.
- W2034626250 cites W2028021946 @default.
- W2034626250 cites W2031091378 @default.
- W2034626250 cites W2038269021 @default.
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- W2034626250 cites W2038937726 @default.
- W2034626250 cites W2045217277 @default.
- W2034626250 cites W2047842452 @default.
- W2034626250 cites W2051690744 @default.
- W2034626250 cites W2052636645 @default.
- W2034626250 cites W2054421559 @default.
- W2034626250 cites W2055922123 @default.
- W2034626250 cites W2057095812 @default.
- W2034626250 cites W2059358614 @default.
- W2034626250 cites W2059665365 @default.
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- W2034626250 cites W2093020443 @default.
- W2034626250 cites W2093909923 @default.
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- W2034626250 cites W2117100312 @default.
- W2034626250 cites W2120954095 @default.
- W2034626250 cites W2126093778 @default.
- W2034626250 cites W2126663182 @default.
- W2034626250 cites W2129011493 @default.
- W2034626250 cites W2142022809 @default.
- W2034626250 cites W2143837695 @default.
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- W2034626250 cites W2178790062 @default.
- W2034626250 cites W2180410797 @default.
- W2034626250 cites W4244561364 @default.
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- W2034626250 cites W4296404860 @default.
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- W2034626250 doi "https://doi.org/10.1210/en.2004-0842" @default.
- W2034626250 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15650079" @default.
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