Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034626351> ?p ?o ?g. }
- W2034626351 endingPage "140" @default.
- W2034626351 startingPage "129" @default.
- W2034626351 abstract "Powerful capillary electrophoresis (CE) methods were developed for monitoring the reaction of ecto-5'-nucleotidase (ecto-5'-NT, CD73), a (patho)biochemically important enzyme that hydrolyzes nucleoside-5'-monophosphates to the corresponding nucleosides. The enzymatic reaction was performed either before injection into the capillary (method A) or directly within the capillary (method B). In method A, separation of substrates and products was achieved within 8 min using an eCAP fused-silica capillary (20 cm effective length, 75 microM i.d., UV detection at 260 nm), 40 mM sodium borate buffer (pH 9.1), normal polarity, and a constant voltage of 15 kV. In method B, the sandwich technique was applied; substrate dissolved in reaction buffer (10mM Hepes [pH 7.4], 2mM MgCl2, and 1mM CaCl2) was hydrodynamically injected into a fused-silica capillary (30 cm, 75 microM i.d.), followed by enzyme (recombinant rat ecto-5'-NT) and subsequent injection of substrate solution. The reaction was initiated by the application of 1 kV voltage for 1 min. The voltage was turned off for 1 min and again turned on at a constant voltage of 15 kV to elute products (nucleosides) within 4 min using borate buffer (40 mM, pH 9.1). Thus, assays could be performed within 6 min, including enzymatic reaction, separation, and quantification of the formed nucleoside. The CE methods were used for measuring enzyme kinetics and for assaying inhibitors and substrates. In addition, the online assay was successfully applied to melanoma cell membrane preparations natively expressing the human ecto-5'-NT." @default.
- W2034626351 created "2016-06-24" @default.
- W2034626351 creator A5017915434 @default.
- W2034626351 creator A5032059683 @default.
- W2034626351 creator A5044354556 @default.
- W2034626351 creator A5050544457 @default.
- W2034626351 creator A5052786858 @default.
- W2034626351 date "2008-02-01" @default.
- W2034626351 modified "2023-10-02" @default.
- W2034626351 title "Capillary electrophoresis-based nanoscale assays for monitoring ecto-5′-nucleotidase activity and inhibition in preparations of recombinant enzyme and melanoma cell membranes" @default.
- W2034626351 cites W1513653241 @default.
- W2034626351 cites W1563328096 @default.
- W2034626351 cites W1596490324 @default.
- W2034626351 cites W1665630 @default.
- W2034626351 cites W1782568954 @default.
- W2034626351 cites W1792542720 @default.
- W2034626351 cites W1972906179 @default.
- W2034626351 cites W1976326730 @default.
- W2034626351 cites W1977494928 @default.
- W2034626351 cites W1978070484 @default.
- W2034626351 cites W1981001845 @default.
- W2034626351 cites W1990580672 @default.
- W2034626351 cites W1994721853 @default.
- W2034626351 cites W2019296019 @default.
- W2034626351 cites W2024082532 @default.
- W2034626351 cites W2025782983 @default.
- W2034626351 cites W2028762032 @default.
- W2034626351 cites W2045214924 @default.
- W2034626351 cites W2045955771 @default.
- W2034626351 cites W2051343837 @default.
- W2034626351 cites W2051987993 @default.
- W2034626351 cites W2054577728 @default.
- W2034626351 cites W2056985354 @default.
- W2034626351 cites W2060227543 @default.
- W2034626351 cites W2060691656 @default.
- W2034626351 cites W2073166260 @default.
- W2034626351 cites W2074640508 @default.
- W2034626351 cites W2081964488 @default.
- W2034626351 cites W2082693377 @default.
- W2034626351 cites W2090391583 @default.
- W2034626351 cites W2094477575 @default.
- W2034626351 cites W2108067308 @default.
- W2034626351 cites W2138049320 @default.
- W2034626351 cites W2143999179 @default.
- W2034626351 cites W2166798770 @default.
- W2034626351 cites W2319423449 @default.
- W2034626351 doi "https://doi.org/10.1016/j.ab.2007.09.028" @default.
- W2034626351 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17980347" @default.
- W2034626351 hasPublicationYear "2008" @default.
- W2034626351 type Work @default.
- W2034626351 sameAs 2034626351 @default.
- W2034626351 citedByCount "40" @default.
- W2034626351 countsByYear W20346263512012 @default.
- W2034626351 countsByYear W20346263512013 @default.
- W2034626351 countsByYear W20346263512014 @default.
- W2034626351 countsByYear W20346263512015 @default.
- W2034626351 countsByYear W20346263512016 @default.
- W2034626351 countsByYear W20346263512018 @default.
- W2034626351 countsByYear W20346263512019 @default.
- W2034626351 countsByYear W20346263512020 @default.
- W2034626351 countsByYear W20346263512021 @default.
- W2034626351 countsByYear W20346263512022 @default.
- W2034626351 countsByYear W20346263512023 @default.
- W2034626351 crossrefType "journal-article" @default.
- W2034626351 hasAuthorship W2034626351A5017915434 @default.
- W2034626351 hasAuthorship W2034626351A5032059683 @default.
- W2034626351 hasAuthorship W2034626351A5044354556 @default.
- W2034626351 hasAuthorship W2034626351A5050544457 @default.
- W2034626351 hasAuthorship W2034626351A5052786858 @default.
- W2034626351 hasConcept C111368507 @default.
- W2034626351 hasConcept C127313418 @default.
- W2034626351 hasConcept C181199279 @default.
- W2034626351 hasConcept C185592680 @default.
- W2034626351 hasConcept C18880352 @default.
- W2034626351 hasConcept C2777289219 @default.
- W2034626351 hasConcept C2777913276 @default.
- W2034626351 hasConcept C2900643 @default.
- W2034626351 hasConcept C41625074 @default.
- W2034626351 hasConcept C43617362 @default.
- W2034626351 hasConcept C55493867 @default.
- W2034626351 hasConceptScore W2034626351C111368507 @default.
- W2034626351 hasConceptScore W2034626351C127313418 @default.
- W2034626351 hasConceptScore W2034626351C181199279 @default.
- W2034626351 hasConceptScore W2034626351C185592680 @default.
- W2034626351 hasConceptScore W2034626351C18880352 @default.
- W2034626351 hasConceptScore W2034626351C2777289219 @default.
- W2034626351 hasConceptScore W2034626351C2777913276 @default.
- W2034626351 hasConceptScore W2034626351C2900643 @default.
- W2034626351 hasConceptScore W2034626351C41625074 @default.
- W2034626351 hasConceptScore W2034626351C43617362 @default.
- W2034626351 hasConceptScore W2034626351C55493867 @default.
- W2034626351 hasIssue "1" @default.
- W2034626351 hasLocation W20346263511 @default.
- W2034626351 hasLocation W20346263512 @default.
- W2034626351 hasOpenAccess W2034626351 @default.
- W2034626351 hasPrimaryLocation W20346263511 @default.
- W2034626351 hasRelatedWork W2006911167 @default.
- W2034626351 hasRelatedWork W2034626351 @default.