Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034632658> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2034632658 endingPage "5306" @default.
- W2034632658 startingPage "5302" @default.
- W2034632658 abstract "I have isolated glucose-6-phosphate dehydrogenase from rabbit liver microsomes and determined its complete amino acid sequence. Sequence determination was achieved by automated Edman degradation of peptides generated by chemical and enzymatic cleavages. The microsomal enzyme consists of 763 residues and is quite dissimilar from the previously characterized cytosolic enzymes. The N terminus of the microsomal enzyme is blocked by a pyroglutamyl residue. Carbohydrate is attached at Asn-138 and Asn-263, implying that the bulk of the protein is oriented on the lumenal side of the endoplasmic membrane. The amino acid sequence of the microsomal protein shows limited homology to the extensively sequenced cytosolic glucose-6-phosphate dehydrogenases. Clusters of up to six identical residues can be identified in four regions: peptide segments at residues 10-21, 154-163, and 173-261. In addition, another array of identical residues, requiring a 100-residue deletion in the sequence of the microsomal enzyme, spans residues 436-462 and corresponds to residues 348-373 of the cytosolic protein. Two segments with a Gly-Xaa-Gly-Xaa-Xaa-Gly motif, related to a coenzyme binding fold, were identified at Gly-399 and Gly-491. In the cytosolic enzymes, a variation of this sequence motif occurs at Gly-37 and Gly-241. The 300-residue C-terminal segment of the microsomal enzyme is unique and has no counterpart in the cytosolic or the bacterial enzymes. An unexpected finding with regard to the microsomal enzyme is that it lacks an identifiable membrane-spanning region or the lumenal-protein C-terminal consensus sequences Lys-Asp-Glu or His-Ile/Thr-Glu-Leu. Thus, the mode of transport and retention of this protein in the lumen of endoplasmic reticulum remains to be determined." @default.
- W2034632658 created "2016-06-24" @default.
- W2034632658 creator A5009100025 @default.
- W2034632658 date "1993-06-01" @default.
- W2034632658 modified "2023-10-16" @default.
- W2034632658 title "Isolation and the complete amino acid sequence of lumenal endoplasmic reticulum glucose-6-phosphate dehydrogenase." @default.
- W2034632658 cites W1017092199 @default.
- W2034632658 cites W1485660887 @default.
- W2034632658 cites W1494346945 @default.
- W2034632658 cites W1495506610 @default.
- W2034632658 cites W1516822474 @default.
- W2034632658 cites W1542327565 @default.
- W2034632658 cites W1551122359 @default.
- W2034632658 cites W1552626264 @default.
- W2034632658 cites W1569239445 @default.
- W2034632658 cites W1579774371 @default.
- W2034632658 cites W1585876465 @default.
- W2034632658 cites W1589721097 @default.
- W2034632658 cites W1611606593 @default.
- W2034632658 cites W1667829589 @default.
- W2034632658 cites W1975304761 @default.
- W2034632658 cites W1990523657 @default.
- W2034632658 cites W2004576172 @default.
- W2034632658 cites W2018729436 @default.
- W2034632658 cites W2021872060 @default.
- W2034632658 cites W2025796657 @default.
- W2034632658 cites W2042561904 @default.
- W2034632658 cites W2047440807 @default.
- W2034632658 cites W2048035978 @default.
- W2034632658 cites W2053213259 @default.
- W2034632658 cites W2087825173 @default.
- W2034632658 cites W2109871327 @default.
- W2034632658 cites W2145835877 @default.
- W2034632658 cites W2198989106 @default.
- W2034632658 cites W2237782819 @default.
- W2034632658 cites W976898132 @default.
- W2034632658 doi "https://doi.org/10.1073/pnas.90.11.5302" @default.
- W2034632658 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/46704" @default.
- W2034632658 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8506377" @default.
- W2034632658 hasPublicationYear "1993" @default.
- W2034632658 type Work @default.
- W2034632658 sameAs 2034632658 @default.
- W2034632658 citedByCount "66" @default.
- W2034632658 countsByYear W20346326582013 @default.
- W2034632658 countsByYear W20346326582014 @default.
- W2034632658 countsByYear W20346326582015 @default.
- W2034632658 countsByYear W20346326582016 @default.
- W2034632658 countsByYear W20346326582017 @default.
- W2034632658 countsByYear W20346326582018 @default.
- W2034632658 crossrefType "journal-article" @default.
- W2034632658 hasAuthorship W2034632658A5009100025 @default.
- W2034632658 hasBestOaLocation W20346326582 @default.
- W2034632658 hasConcept C104317684 @default.
- W2034632658 hasConcept C158617107 @default.
- W2034632658 hasConcept C167625842 @default.
- W2034632658 hasConcept C181199279 @default.
- W2034632658 hasConcept C2776317432 @default.
- W2034632658 hasConcept C515207424 @default.
- W2034632658 hasConcept C55493867 @default.
- W2034632658 hasConcept C86803240 @default.
- W2034632658 hasConcept C87644729 @default.
- W2034632658 hasConcept C87933860 @default.
- W2034632658 hasConcept C98539663 @default.
- W2034632658 hasConceptScore W2034632658C104317684 @default.
- W2034632658 hasConceptScore W2034632658C158617107 @default.
- W2034632658 hasConceptScore W2034632658C167625842 @default.
- W2034632658 hasConceptScore W2034632658C181199279 @default.
- W2034632658 hasConceptScore W2034632658C2776317432 @default.
- W2034632658 hasConceptScore W2034632658C515207424 @default.
- W2034632658 hasConceptScore W2034632658C55493867 @default.
- W2034632658 hasConceptScore W2034632658C86803240 @default.
- W2034632658 hasConceptScore W2034632658C87644729 @default.
- W2034632658 hasConceptScore W2034632658C87933860 @default.
- W2034632658 hasConceptScore W2034632658C98539663 @default.
- W2034632658 hasIssue "11" @default.
- W2034632658 hasLocation W20346326581 @default.
- W2034632658 hasLocation W20346326582 @default.
- W2034632658 hasLocation W20346326583 @default.
- W2034632658 hasLocation W20346326584 @default.
- W2034632658 hasOpenAccess W2034632658 @default.
- W2034632658 hasPrimaryLocation W20346326581 @default.
- W2034632658 hasRelatedWork W1644178486 @default.
- W2034632658 hasRelatedWork W192390236 @default.
- W2034632658 hasRelatedWork W1977200340 @default.
- W2034632658 hasRelatedWork W1988845818 @default.
- W2034632658 hasRelatedWork W1991358375 @default.
- W2034632658 hasRelatedWork W2016062340 @default.
- W2034632658 hasRelatedWork W2030492692 @default.
- W2034632658 hasRelatedWork W2073375101 @default.
- W2034632658 hasRelatedWork W2083360052 @default.
- W2034632658 hasRelatedWork W2116312818 @default.
- W2034632658 hasVolume "90" @default.
- W2034632658 isParatext "false" @default.
- W2034632658 isRetracted "false" @default.
- W2034632658 magId "2034632658" @default.
- W2034632658 workType "article" @default.