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• W2034635074 abstract "A high-performance liquid chromatography method was established for simultaneously determining seven major components, i.e. protocatechuic aldehyde, notoginsenoside R1, ginsenoside Rg1, salvianolic acid B, ginsenoside Rb1, cryptotanshinone and tanshinone IIA in Fufang Danshen tablet, a commonly used traditional Chinese medicinal combined prescription mainly derived from the roots of Salvia miltiorrhiza and Panax notoginseng. These seven compounds, belonging to the chemical types of phenolic acids, diterpenoid quinones and saponins, were simultaneously separated on Zorbax C18 column (250 × 4.6 mm, 5.0 µm) with the column temperature at 30°C. The mobile phase was composed of (A) aqueous phosphoric acid (0.1%, v/v) and (B) acetonitrile using a gradient elution of 7–17% B at 0–10 min, 17–20% B at 10–12 min, 20–21% B at 12–16 min, 21% B at 16–32 min, 21–29% B at 32–40 min, 29–35% B at 40–55 min, 35–65% B at 55–65 min and 65–80% B at 65–80 min; the flow rate was 1.0 mL/min. Detection wavelengths were set at 203 nm for notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1, 281 nm for protocatechuic aldehyde, salvianolic acid B, and 270 nm for cryptotanshinone and tanshinone IIA. All calibration curves showed good linear regression (r2 > 0.9992) within test ranges. The established method showed good precision and accuracy with overall intra-day and inter-day variations of 0.15–4.35 and 0.61–5.17% respectively, and overall recoveries of 94.8–102.1% for the seven compounds analyzed. The developed method has been successfully applied to simultaneous evaluation of the intrinsic quality of both Danshen and Sanqi in Fufang Danshen tablets from different pharmaceutical companies. Copyright © 2006 John Wiley & Sons, Ltd." @default.
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• W2034635074 date "2006-01-01" @default.
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• W2034635074 title "Simultaneous determination of seven active components of Fufang Danshen tablet by high performance liquid chromatography" @default.
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• W2034635074 doi "https://doi.org/10.1002/bmc.700" @default.
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