FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034643133> ?p ?o ?g. }

• W2034643133 endingPage "683" @default.
• W2034643133 startingPage "675" @default.
• W2034643133 abstract "Abstract Elevation of lactate, ammonia, osmolality, and carbon dioxide to inhibitory levels was reported to have adverse effects on cell growth and protein productivity in mammalian cell culture. Multivariate analysis methods were used to investigate the roles of these repressing metabolites in a fed‐batch CHO cell culture for antibody fusion protein B1 (B1) production. Principal Factor Analysis methodology was applied to manufacturing‐scale data of 112 cell culture runs, which identified threshold values of four repressing metabolites as follows: (1) ammonium levels above 5.1 mM inhibit cell growth; (2) both lactate and osmolality levels above 58 mM and 382 mOsm/kg affect cell viability; and (3) carbon dioxide levels at or above 111 mmHg reduce protein quality. These threshold values were then verified by simulations using Monod‐type equations and Canonical Correlation. These results suggest that adverse effects on cell growth, productivity, and product quality may be minimized under the ideal cell culture condition, in which the peak values of all four repressing metabolites are maintained below the threshold values. This strategy was evaluated in 45 cell culture runs in 50‐L bioreactors. Eight out of 45 runs were operated under the ideal condition, while the remaining 37 runs had at least one repressing metabolite with peak value at or above the threshold. In comparison to the remaining runs, the eight cell culture runs under the ideal condition had 17%, 40%, and 11% higher values in peak viable cell density, final B1 titer, and quality attribute, respectively. The unique methodology used in this study may be generally applicable in characterizing cell culture processes." @default.
• W2034643133 created "2016-06-24" @default.
• W2034643133 creator A5019233309 @default.
• W2034643133 creator A5032956379 @default.
• W2034643133 creator A5044728247 @default.
• W2034643133 creator A5056270028 @default.
• W2034643133 date "2008-05-01" @default.
• W2034643133 modified "2023-10-15" @default.
• W2034643133 title "Identifying Inhibitory Threshold Values of Repressing Metabolites in CHO Cell Culture Using Multivariate Analysis Methods" @default.
• W2034643133 cites W1575974382 @default.
• W2034643133 cites W1965185630 @default.
• W2034643133 cites W1981260512 @default.
• W2034643133 cites W1983480646 @default.
• W2034643133 cites W1995250181 @default.
• W2034643133 cites W1995932419 @default.
• W2034643133 cites W1999276310 @default.
• W2034643133 cites W2006436496 @default.
• W2034643133 cites W2016937028 @default.
• W2034643133 cites W2017287753 @default.
• W2034643133 cites W2018033335 @default.
• W2034643133 cites W2020840252 @default.
• W2034643133 cites W2021351846 @default.
• W2034643133 cites W2023178000 @default.
• W2034643133 cites W2027630798 @default.
• W2034643133 cites W2039966214 @default.
• W2034643133 cites W2042716641 @default.
• W2034643133 cites W2054534751 @default.
• W2034643133 cites W2055062956 @default.
• W2034643133 cites W2061226704 @default.
• W2034643133 cites W2064983459 @default.
• W2034643133 cites W2066174543 @default.
• W2034643133 cites W2069349248 @default.
• W2034643133 cites W2075261924 @default.
• W2034643133 cites W2087058811 @default.
• W2034643133 cites W2089159507 @default.
• W2034643133 cites W2093910499 @default.
• W2034643133 cites W2098201181 @default.
• W2034643133 cites W2111215449 @default.
• W2034643133 cites W2118540273 @default.
• W2034643133 cites W2119641678 @default.
• W2034643133 cites W2132990040 @default.
• W2034643133 cites W2151077707 @default.
• W2034643133 cites W2154496417 @default.
• W2034643133 doi "https://doi.org/10.1021/bp070466m" @default.
• W2034643133 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18422365" @default.
• W2034643133 hasPublicationYear "2008" @default.
• W2034643133 type Work @default.
• W2034643133 sameAs 2034643133 @default.
• W2034643133 citedByCount "46" @default.
• W2034643133 countsByYear W20346431332012 @default.
• W2034643133 countsByYear W20346431332013 @default.
• W2034643133 countsByYear W20346431332014 @default.
• W2034643133 countsByYear W20346431332015 @default.
• W2034643133 countsByYear W20346431332016 @default.
• W2034643133 countsByYear W20346431332017 @default.
• W2034643133 countsByYear W20346431332018 @default.
• W2034643133 countsByYear W20346431332019 @default.
• W2034643133 countsByYear W20346431332020 @default.
• W2034643133 countsByYear W20346431332021 @default.
• W2034643133 crossrefType "journal-article" @default.
• W2034643133 hasAuthorship W2034643133A5019233309 @default.
• W2034643133 hasAuthorship W2034643133A5032956379 @default.
• W2034643133 hasAuthorship W2034643133A5044728247 @default.
• W2034643133 hasAuthorship W2034643133A5056270028 @default.
• W2034643133 hasBestOaLocation W20346431331 @default.
• W2034643133 hasConcept C1491633281 @default.
• W2034643133 hasConcept C185592680 @default.
• W2034643133 hasConcept C18903297 @default.
• W2034643133 hasConcept C2777477808 @default.
• W2034643133 hasConcept C43617362 @default.
• W2034643133 hasConcept C530467964 @default.
• W2034643133 hasConcept C54355233 @default.
• W2034643133 hasConcept C55493867 @default.
• W2034643133 hasConcept C62112901 @default.
• W2034643133 hasConcept C81885089 @default.
• W2034643133 hasConcept C86803240 @default.
• W2034643133 hasConceptScore W2034643133C1491633281 @default.
• W2034643133 hasConceptScore W2034643133C185592680 @default.
• W2034643133 hasConceptScore W2034643133C18903297 @default.
• W2034643133 hasConceptScore W2034643133C2777477808 @default.
• W2034643133 hasConceptScore W2034643133C43617362 @default.
• W2034643133 hasConceptScore W2034643133C530467964 @default.
• W2034643133 hasConceptScore W2034643133C54355233 @default.
• W2034643133 hasConceptScore W2034643133C55493867 @default.
• W2034643133 hasConceptScore W2034643133C62112901 @default.
• W2034643133 hasConceptScore W2034643133C81885089 @default.
• W2034643133 hasConceptScore W2034643133C86803240 @default.
• W2034643133 hasIssue "3" @default.
• W2034643133 hasLocation W20346431331 @default.
• W2034643133 hasLocation W20346431332 @default.
• W2034643133 hasOpenAccess W2034643133 @default.
• W2034643133 hasPrimaryLocation W20346431331 @default.
• W2034643133 hasRelatedWork W1985998670 @default.
• W2034643133 hasRelatedWork W2072481810 @default.
• W2034643133 hasRelatedWork W2083693850 @default.
• W2034643133 hasRelatedWork W2143718611 @default.
• W2034643133 hasRelatedWork W2320661246 @default.
• W2034643133 hasRelatedWork W2347992620 @default.

• next