FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034651925> ?p ?o ?g. }

• W2034651925 endingPage "e84070" @default.
• W2034651925 startingPage "e84070" @default.
• W2034651925 abstract "Introduction In contrast to the defence mechanism of RNA interference (RNAi) in plants and invertebrates, its role in the innate response to virus infection of mammals is a matter of debate. Since RNAi has a well-established role in controlling infection of the alphavirus Sindbis virus (SINV) in insects, we have used this virus to investigate the role of RNAi in SINV infection of human cells. Results SINV AR339 and TR339-GFP were adapted to grow in HEK293 cells. Deep sequencing of small RNAs (sRNAs) early in SINV infection (4 and 6 hpi) showed low abundance (0.8%) of viral sRNAs (vsRNAs), with no size, sequence or location specific patterns characteristic of Dicer products nor did they possess any discernible pattern to ascribe to a specific RNAi biogenesis pathway. This was supported by multiple variants for each sequence, and lack of hot spots along the viral genome sequence. The abundance of the best defined vsRNAs was below the limit of Northern blot detection. The adaptation of the virus to HEK293 cells showed little sequence changes compared to the reference; however, a SNP in E1 gene with a preference from G to C was found. Deep sequencing results showed little variation of expression of cellular microRNAs (miRNAs) at 4 and 6 hpi compared to uninfected cells. Twelve miRNAs exhibiting some minor differential expression by sequencing, showed no difference in expression by Northern blot analysis. Conclusions We show that, unlike SINV infection of invertebrates, generation of Dicer-dependent svRNAs and change in expression of cellular miRNAs were not detected as part of the Human response to SINV." @default.
• W2034651925 created "2016-06-24" @default.
• W2034651925 creator A5008262717 @default.
• W2034651925 creator A5053059503 @default.
• W2034651925 creator A5075011438 @default.
• W2034651925 creator A5085848606 @default.
• W2034651925 date "2013-12-31" @default.
• W2034651925 modified "2023-10-18" @default.
• W2034651925 title "Small RNA Analysis in Sindbis Virus Infected Human HEK293 Cells" @default.
• W2034651925 cites W1566730797 @default.
• W2034651925 cites W1885622104 @default.
• W2034651925 cites W1965893507 @default.
• W2034651925 cites W1968552702 @default.
• W2034651925 cites W1970219417 @default.
• W2034651925 cites W1975319407 @default.
• W2034651925 cites W1985313214 @default.
• W2034651925 cites W1987679698 @default.
• W2034651925 cites W1989129419 @default.
• W2034651925 cites W2005476363 @default.
• W2034651925 cites W2010970638 @default.
• W2034651925 cites W2012728850 @default.
• W2034651925 cites W2014542764 @default.
• W2034651925 cites W2017324476 @default.
• W2034651925 cites W2017481656 @default.
• W2034651925 cites W2020996714 @default.
• W2034651925 cites W2021618307 @default.
• W2034651925 cites W2026772883 @default.
• W2034651925 cites W2028543950 @default.
• W2034651925 cites W2039687496 @default.
• W2034651925 cites W2040308682 @default.
• W2034651925 cites W2047781063 @default.
• W2034651925 cites W2049449836 @default.
• W2034651925 cites W2050444114 @default.
• W2034651925 cites W2055063562 @default.
• W2034651925 cites W2061742276 @default.
• W2034651925 cites W2067903724 @default.
• W2034651925 cites W2069041912 @default.
• W2034651925 cites W2070571588 @default.
• W2034651925 cites W2071670416 @default.
• W2034651925 cites W2072442043 @default.
• W2034651925 cites W2076014548 @default.
• W2034651925 cites W2080413744 @default.
• W2034651925 cites W2090085683 @default.
• W2034651925 cites W2091666608 @default.
• W2034651925 cites W2103903744 @default.
• W2034651925 cites W2110870493 @default.
• W2034651925 cites W2111585311 @default.
• W2034651925 cites W2115503551 @default.
• W2034651925 cites W2115618792 @default.
• W2034651925 cites W2117092553 @default.
• W2034651925 cites W2118526609 @default.
• W2034651925 cites W2122550126 @default.
• W2034651925 cites W2123364539 @default.
• W2034651925 cites W2130679719 @default.
• W2034651925 cites W2133924996 @default.
• W2034651925 cites W2137988707 @default.
• W2034651925 cites W2143578456 @default.
• W2034651925 cites W2145970005 @default.
• W2034651925 cites W2147908409 @default.
• W2034651925 cites W2148400529 @default.
• W2034651925 cites W2152854377 @default.
• W2034651925 cites W2154431984 @default.
• W2034651925 cites W2155044281 @default.
• W2034651925 cites W2159746639 @default.
• W2034651925 cites W2169416219 @default.
• W2034651925 cites W2170889480 @default.
• W2034651925 cites W2612812586 @default.
• W2034651925 doi "https://doi.org/10.1371/journal.pone.0084070" @default.
• W2034651925 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3877139" @default.
• W2034651925 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24391886" @default.
• W2034651925 hasPublicationYear "2013" @default.
• W2034651925 type Work @default.
• W2034651925 sameAs 2034651925 @default.
• W2034651925 citedByCount "11" @default.
• W2034651925 countsByYear W20346519252014 @default.
• W2034651925 countsByYear W20346519252015 @default.
• W2034651925 countsByYear W20346519252017 @default.
• W2034651925 countsByYear W20346519252019 @default.
• W2034651925 countsByYear W20346519252020 @default.
• W2034651925 countsByYear W20346519252021 @default.
• W2034651925 crossrefType "journal-article" @default.
• W2034651925 hasAuthorship W2034651925A5008262717 @default.
• W2034651925 hasAuthorship W2034651925A5053059503 @default.
• W2034651925 hasAuthorship W2034651925A5075011438 @default.
• W2034651925 hasAuthorship W2034651925A5085848606 @default.
• W2034651925 hasBestOaLocation W20346519251 @default.
• W2034651925 hasConcept C104317684 @default.
• W2034651925 hasConcept C132917006 @default.
• W2034651925 hasConcept C141231307 @default.
• W2034651925 hasConcept C145059251 @default.
• W2034651925 hasConcept C153911025 @default.
• W2034651925 hasConcept C159047783 @default.
• W2034651925 hasConcept C166703698 @default.
• W2034651925 hasConcept C174510640 @default.
• W2034651925 hasConcept C2522874641 @default.
• W2034651925 hasConcept C2777343345 @default.
• W2034651925 hasConcept C2777474056 @default.
• W2034651925 hasConcept C2779867133 @default.

• next