Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034658407> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W2034658407 endingPage "254" @default.
- W2034658407 startingPage "251" @default.
- W2034658407 abstract "The impact of nanometer-scale plastics (<1000 nm nanoplastics, NPs) on the bioaccumulation of hydrophobic organic pollutants, and especially polycyclic aromatic hydrocarbons (PAHs), in marine organisms has become of urgent concern. However, simultaneous determinations of the bioaccumulation of NPs and PAHs have been hindered by the lack of an efficient digestion method that removes background interference from the tissue without altering the surface properties of the plastic and destroying the PAHs. To solve this problem, an enzymatic digestion-based protocol using proteinase K and subsequent quantification methods were developed on a typical marine benthic invertebrate – the clamworm Perinereis aibuhitensis. Enzymatic digestion removed 91% of the biological tissues, comparable to the amount removed using 65% HNO3 (93% removed) and better than that removed using 30% H2O2 or 10% KOH digestion (76% and 66%, respectively). After enzymatic digestion, roughly 92% of the NPs and 88% of the amount of pyrene were recovered, without significant modification of the NPs or pyrene degradation. By contrast, the NP and pyrene recovery achieved with HNO3 digestion was only 1.4% and 0.1%, respectively. The newly developed protocol was successfully applied to a 96-h bioaccumulation study. The use of radioactively labeled 14C-pyrene and fluorescently labeled NPs allowed the simultaneous quantification of NPs and PAHs in the clamworm and revealed a bioconcentration factor (BCF) of 1.96 ± 0.93 and 402.7 ± 47.0, respectively. The quantification of NPs and pyrene indicated that NP-adsorbed pyrene accounted for <1% of the total pyrene accumulation in the clamworm body when the concentration of NPs in seawater was as low as 0.4 mg/L. Our enzymatic digestion and dual-labeling technique thus provides the first reported BCF value of NPs in a marine benthic organism and new insights into the vector effects of these particles on the bioaccumulation of organic contaminants in a marine ecosystem." @default.
- W2034658407 created "2016-06-24" @default.
- W2034658407 creator A5072304156 @default.
- W2034658407 date "1964-02-01" @default.
- W2034658407 modified "2023-09-23" @default.
- W2034658407 title "Nitric acid digestion of tissues for liquid scintillation counting" @default.
- W2034658407 cites W2000685072 @default.
- W2034658407 cites W2031057078 @default.
- W2034658407 cites W2033912656 @default.
- W2034658407 doi "https://doi.org/10.1016/0003-2697(64)90236-2" @default.
- W2034658407 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14134441" @default.
- W2034658407 hasPublicationYear "1964" @default.
- W2034658407 type Work @default.
- W2034658407 sameAs 2034658407 @default.
- W2034658407 citedByCount "31" @default.
- W2034658407 countsByYear W20346584072012 @default.
- W2034658407 countsByYear W20346584072013 @default.
- W2034658407 countsByYear W20346584072019 @default.
- W2034658407 crossrefType "journal-article" @default.
- W2034658407 hasAuthorship W2034658407A5072304156 @default.
- W2034658407 hasConcept C107872376 @default.
- W2034658407 hasConcept C178790620 @default.
- W2034658407 hasConcept C185592680 @default.
- W2034658407 hasConcept C2777538855 @default.
- W2034658407 hasConcept C2778951431 @default.
- W2034658407 hasConcept C2779229383 @default.
- W2034658407 hasConcept C2781357212 @default.
- W2034658407 hasConcept C43617362 @default.
- W2034658407 hasConcept C71572567 @default.
- W2034658407 hasConcept C82685317 @default.
- W2034658407 hasConceptScore W2034658407C107872376 @default.
- W2034658407 hasConceptScore W2034658407C178790620 @default.
- W2034658407 hasConceptScore W2034658407C185592680 @default.
- W2034658407 hasConceptScore W2034658407C2777538855 @default.
- W2034658407 hasConceptScore W2034658407C2778951431 @default.
- W2034658407 hasConceptScore W2034658407C2779229383 @default.
- W2034658407 hasConceptScore W2034658407C2781357212 @default.
- W2034658407 hasConceptScore W2034658407C43617362 @default.
- W2034658407 hasConceptScore W2034658407C71572567 @default.
- W2034658407 hasConceptScore W2034658407C82685317 @default.
- W2034658407 hasIssue "2" @default.
- W2034658407 hasLocation W20346584071 @default.
- W2034658407 hasLocation W20346584072 @default.
- W2034658407 hasOpenAccess W2034658407 @default.
- W2034658407 hasPrimaryLocation W20346584071 @default.
- W2034658407 hasRelatedWork W1989434189 @default.
- W2034658407 hasRelatedWork W1989543376 @default.
- W2034658407 hasRelatedWork W2009224023 @default.
- W2034658407 hasRelatedWork W2034658407 @default.
- W2034658407 hasRelatedWork W2053625486 @default.
- W2034658407 hasRelatedWork W2108686416 @default.
- W2034658407 hasRelatedWork W2116702967 @default.
- W2034658407 hasRelatedWork W2123856720 @default.
- W2034658407 hasRelatedWork W2130256304 @default.
- W2034658407 hasRelatedWork W4245223462 @default.
- W2034658407 hasVolume "7" @default.
- W2034658407 isParatext "false" @default.
- W2034658407 isRetracted "false" @default.
- W2034658407 magId "2034658407" @default.
- W2034658407 workType "article" @default.