Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034658569> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2034658569 endingPage "1211" @default.
- W2034658569 startingPage "1201" @default.
- W2034658569 abstract "A phylogenetically conserved guanine·uracil (G·U) pair defines the 5‘-exon/intron boundary of precursor RNAs containing group I introns. In this wobble base pair, the G forms two hydrogen bonds with U in a base pairing geometry shifted from that of a canonical Watson−Crick pair. On the basis of thermodynamic measurements of synthetic base pair analogs (inosine, diaminopurine riboside, guanosine, or adenosine paired with U, C, or isocytidine) in place of the G·U pair, we have previously reported that the N2 exocyclic amine of the G is important for docking the 5‘-exon into the active site of the Tetrahymena ribozyme [Strobel, S. A., & Cech, T. R. (1995) Science 267, 675−679]. Here we describe kinetic characterization of ribozyme−substrate combinations containing the same series of analogs. By measuring the rate constants of 5‘-exon miscleavage (cleavage at incorrect phosphates), we demonstrate that the 5‘-exon/intron boundary is primarily defined by the exocyclic amine of the G. The amine makes its contribution (2.5 kcal·mol-1) in the context of all three wobble pairs tested but fails to make a significant contribution (<0.8 kcal·mol-1) when presented in a Watson−Crick base pairing geometry. We also demonstrate that the exocyclic amine makes a modest contribution to chemical transition state stabilization (1.0 kcal·mol-1 relative to an inosine−U pair). The majority of this transition state contribution (0.7 kcal·mol-1) is independent of that contributed by the 2‘-hydroxyl of the neighboring U. This argues against the model in which substantial transition state stabilization is derived from a water molecule bridging between the exocyclic amine of G and the 2‘-hydroxyl of U. Instead it suggests that the tertiary interaction between the exocyclic amine and its hydrogen bonding partner in the active site is slightly improved during the chemical transition. We conclude that the exocyclic amine of G is the primary contributor to many characteristics of reactivity that have been ascribed to the conserved G·U pair, including stabilization of the chemical transition state and definition of the 5‘-exon/intron boundary." @default.
- W2034658569 created "2016-06-24" @default.
- W2034658569 creator A5017573996 @default.
- W2034658569 creator A5039902482 @default.
- W2034658569 date "1996-01-01" @default.
- W2034658569 modified "2023-10-17" @default.
- W2034658569 title "Exocyclic Amine of the Conserved G·U Pair at the Cleavage Site of the <i>Tetrahymena</i> Ribozyme Contributes to 5‘-Splice Site Selection and Transition State Stabilization" @default.
- W2034658569 cites W1573511081 @default.
- W2034658569 cites W1975744482 @default.
- W2034658569 cites W2002009265 @default.
- W2034658569 cites W2016791718 @default.
- W2034658569 cites W2035783320 @default.
- W2034658569 doi "https://doi.org/10.1021/bi952244f" @default.
- W2034658569 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8573575" @default.
- W2034658569 hasPublicationYear "1996" @default.
- W2034658569 type Work @default.
- W2034658569 sameAs 2034658569 @default.
- W2034658569 citedByCount "42" @default.
- W2034658569 countsByYear W20346585692012 @default.
- W2034658569 countsByYear W20346585692013 @default.
- W2034658569 countsByYear W20346585692014 @default.
- W2034658569 countsByYear W20346585692015 @default.
- W2034658569 countsByYear W20346585692016 @default.
- W2034658569 countsByYear W20346585692020 @default.
- W2034658569 countsByYear W20346585692021 @default.
- W2034658569 countsByYear W20346585692022 @default.
- W2034658569 crossrefType "journal-article" @default.
- W2034658569 hasAuthorship W2034658569A5017573996 @default.
- W2034658569 hasAuthorship W2034658569A5039902482 @default.
- W2034658569 hasConcept C104317684 @default.
- W2034658569 hasConcept C107745601 @default.
- W2034658569 hasConcept C153957851 @default.
- W2034658569 hasConcept C185581394 @default.
- W2034658569 hasConcept C185592680 @default.
- W2034658569 hasConcept C203353237 @default.
- W2034658569 hasConcept C2776991684 @default.
- W2034658569 hasConcept C2777610669 @default.
- W2034658569 hasConcept C552990157 @default.
- W2034658569 hasConcept C55493867 @default.
- W2034658569 hasConcept C66144319 @default.
- W2034658569 hasConcept C67705224 @default.
- W2034658569 hasConcept C71240020 @default.
- W2034658569 hasConcept C76346623 @default.
- W2034658569 hasConcept C8010536 @default.
- W2034658569 hasConceptScore W2034658569C104317684 @default.
- W2034658569 hasConceptScore W2034658569C107745601 @default.
- W2034658569 hasConceptScore W2034658569C153957851 @default.
- W2034658569 hasConceptScore W2034658569C185581394 @default.
- W2034658569 hasConceptScore W2034658569C185592680 @default.
- W2034658569 hasConceptScore W2034658569C203353237 @default.
- W2034658569 hasConceptScore W2034658569C2776991684 @default.
- W2034658569 hasConceptScore W2034658569C2777610669 @default.
- W2034658569 hasConceptScore W2034658569C552990157 @default.
- W2034658569 hasConceptScore W2034658569C55493867 @default.
- W2034658569 hasConceptScore W2034658569C66144319 @default.
- W2034658569 hasConceptScore W2034658569C67705224 @default.
- W2034658569 hasConceptScore W2034658569C71240020 @default.
- W2034658569 hasConceptScore W2034658569C76346623 @default.
- W2034658569 hasConceptScore W2034658569C8010536 @default.
- W2034658569 hasIssue "4" @default.
- W2034658569 hasLocation W20346585691 @default.
- W2034658569 hasLocation W20346585692 @default.
- W2034658569 hasOpenAccess W2034658569 @default.
- W2034658569 hasPrimaryLocation W20346585691 @default.
- W2034658569 hasRelatedWork W1607687794 @default.
- W2034658569 hasRelatedWork W2006140659 @default.
- W2034658569 hasRelatedWork W2009723196 @default.
- W2034658569 hasRelatedWork W2029667257 @default.
- W2034658569 hasRelatedWork W2052928878 @default.
- W2034658569 hasRelatedWork W2055702166 @default.
- W2034658569 hasRelatedWork W2067162089 @default.
- W2034658569 hasRelatedWork W2085851730 @default.
- W2034658569 hasRelatedWork W2162420094 @default.
- W2034658569 hasRelatedWork W2379344035 @default.
- W2034658569 hasVolume "35" @default.
- W2034658569 isParatext "false" @default.
- W2034658569 isRetracted "false" @default.
- W2034658569 magId "2034658569" @default.
- W2034658569 workType "article" @default.