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• W2034659103 abstract "Objective: E. coliO157:H7 may cause hemorrhagic colitis resembling ischemic colitis. Diagnosis is usually made by finding sorbitol-negative colonies on MacConkey agar that react with O157 and H7 antisera. Most ischemic colitis is idiopathic, but some may be caused byE. coliO157:H7, inasmuch as this organism can produce fibrin thrombi in colon vasculature. The objectives of this study were to determine whetherE. coliO157:H7 infection can be diagnosed retrospectively from paraffin blocks of colon sections and whether an association exists betweenE. coliO157:H7 infection and colonic ischemia. Methods: Paraffin-embedded sections of normal colon (n = 2) and various colitides [ischemic (n = 11),E. coliO157:H7 (n = 2), IBD (n = 8) and pseudomembranous (n = 3)] were used. Sections were deparaffinized, rehydrated, incubated with 3% peroxide in methanol, rinsed, and incubated with peroxidase-labeled antibody isolated from goats immunized with wholeE. coliO157:H7. Sections were stained with peroxidase chromagen reagent and counterstained with hematoxylin. Coarse, granular, orange-brown staining was considered positive. To determine the localization of the chromagen deposits, three cases that stained positive, including one of the culture-provedE. coliO157:H7 colitis and two of colonic ischemia, were processed for electron microscopy. Results: Both cases (100%) ofE. coliO157:H7 colitis and three of 11 (27.3%) cases of ischemic colitis stained positive by light microscopy. In one culture-proved case, electron microscopy demonstrated staining of bacillary structures; in two cases of colonic ischemia, extensive deposits of chromagen material were present that were associated neither with inflammatory cells nor with bacterial forms. Conclusions: Immunoperoxidase staining of archival sections may be used to diagnoseE. coliO157:H7 infection. An etiological role for this organism is possible in some cases of colonic ischemia." @default.
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• W2034659103 date "1998-07-01" @default.
• W2034659103 modified "2023-09-23" @default.
• W2034659103 title "The Immunohistological Diagnosis of E. Coli O157:H7 Colitis: Possible Association With Colonic Ischemia" @default.
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• W2034659103 doi "https://doi.org/10.1111/j.1572-0241.1998.00328.x" @default.
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