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• W2034664543 startingPage "431" @default.
• W2034664543 abstract "Studies comparing the binding of genuine cobalamin (vitamin B12) to that of its natural or synthetic analogues have long established increasing ligand specificity in the order haptocorrin, transcobalamin and intrinsic factor, the high-affinity binding proteins involved in cobalamin transport in mammals. In the present study, ligand specificity was investigated from a structural point of view, for which comparative models of intrinsic factor and haptocorrin are produced based on the crystal structure of the homologous transcobalamin and validated by results of published binding assays. Many interactions between cobalamin and its binding site in the interface of the two domains are conserved among the transporters. A structural comparison suggests that the determinant of specificity regarding cobalamin ligands with modified nucleotide moiety resides in the beta-hairpin motif beta3-turn-beta4 of the smaller C-terminal domain. In haptocorrin, it provides hydrophobic contacts to the benzimidazole moiety through the apolar regions of Arg357, Trp359 and Tyr362. Together, these large side chains may compensate for the missing nucleotide upon cobinamide binding. Intrinsic factor possesses only the tryptophan residue and transcobalamin only the tyrosine residue, consistent with their low affinity for cobinamide. Relative affinity constants for other analogues are rationalized similarly by analysis of steric and electrostatic interactions with the three transporters. The structures also indicate that the C-terminal domain is the first site of cobalamin-binding since part of the beta-hairpin motif is trapped between the nucleotide moiety and the N-terminal domain in the final holo-proteins." @default.
• W2034664543 created "2016-06-24" @default.
• W2034664543 creator A5000843837 @default.
• W2034664543 creator A5024376344 @default.
• W2034664543 creator A5084279871 @default.
• W2034664543 date "2007-04-12" @default.
• W2034664543 modified "2023-09-26" @default.
• W2034664543 title "Structural study on ligand specificity of human vitamin B12 transporters" @default.
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• W2034664543 doi "https://doi.org/10.1042/bj20061394" @default.
• W2034664543 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1876373" @default.
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• W2034664543 hasPublicationYear "2007" @default.
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