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- W2034666566 abstract "Immature inflorescence segments of sugarcane (Saccharum spp. hybrids) breeding lines were cultured on several versions of MS based media containing various concentrations and combinations of 2,4-D, kinetin, BAP, CH and calcium pantothenate. The experimental design involved 1) three pollen developmental stages, 2) six preculture cold treatments, 3) four media, and 4) three genotypes (breeding lines: 87-588, 87-693 and 87-696). Calli could be initiated from all parts of the inflorescence segments. In terms of callusing frequency, the most important factor was the age of the explants. As long as it was at the period of pollen mother cell to tetrad, all of the three genotypes were capable of producing a large quantity of callus. Cold (13 °C) storage for 2 or 3 days enhanced callus frequency significantly (p < 0.05) higher than natural night temperature-treatment. The four culture media produced similar results, except the MSC-GC, which gave rise to the lowest response. The callus produced by genotypes 87-693 and 87-696 on a MS based medium supplemented with CH and glutamine was characterized as watery white, mucilaginous and nodular. The callus derived from 87-588 was light yellow and compact. The former regenerated plants by way of embryogenesis, whereas the pattern of plant regeneration of the latter was through an atypical embryogenesis--organogenesis. All of the calli were highly regenerative (90–100 %) at the 3rd passage. The regenerants were phenotypically normal." @default.
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- W2034666566 date "1993-06-01" @default.
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- W2034666566 title "Factors Affecting Induction, Somatic Embryogenesis and Plant Regeneration of Callus from Cultured Immature Inflorescences of Sugarcane" @default.
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- W2034666566 doi "https://doi.org/10.1016/s0176-1617(11)81580-4" @default.
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