Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034666807> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2034666807 endingPage "13989" @default.
- W2034666807 startingPage "13980" @default.
- W2034666807 abstract "Peroxidases typically bind their reducing substrates weakly, with Kd values in the millimolar range. The binding of benzhydroxamic acid (BHA) to ferric horseradish peroxidase isoenzyme C (HRPC) [Kd = 2.4 μM; Schonbaum, G. R. (1973) J. Biol. Chem. 248, 502−511] is a notable exception and has provided a useful tool for probing the environment of the peroxidase aromatic-donor-binding site and the distal heme cavity. Knowledge of the underlying thermodynamic driving forces is key to understanding the roles of the various H-bonding and hydrophobic interactions in substrate binding. The isothermal titration calorimetry results of this study on the binding of aromatic hydroxamic acid analogues to ferric HRPC under nonturnover conditions (no H2O2 present) confirm the significance of H-bonding interactions in the distal heme cavity in complex stabilization. For example, the binding of BHA to HRPC is enthalpically driven at pH 7.0, with the H-bond to the distal Arg38 providing the largest contribution (6.74 kcal/mol) to the binding energy. The overall relatively weak binding of the hydroxamic acid analogues to HRPC is due to large entropic barriers (−11.3 to −37.9 eu) around neutral pH, with the distal Arg38 acting as an “entropic gate keeper”. Dramatic enthalpy−entropy compensation is observed for BHA and 2-naphthohydroxamic acid binding to HRPC at pH 4.0. The enthalpic loss and entropic gain are likely due to increased flexibility of Arg38 in the complexes at low pH and greater access by water to the active site. Since the Soret absorption band of HRPC is a sensitive probe of the binding of hydroxamic acids and their analogues, it was used to investigate the binding of six donor substrates over the pH range of 4−12. The negligible pH dependence of the Kd values corrected for substrate ionization suggests that enthalpy−entropy compensation is operative over a wide pH range. Examination of the thermodynamics of binding of ring-substituted hyrazides to HRPC reveals that the binding affinities of aromatic donors are highly sensitive to the position and nature of the ring substituent." @default.
- W2034666807 created "2016-06-24" @default.
- W2034666807 creator A5004492579 @default.
- W2034666807 creator A5057514326 @default.
- W2034666807 creator A5062598918 @default.
- W2034666807 creator A5064592336 @default.
- W2034666807 date "2001-10-27" @default.
- W2034666807 modified "2023-09-27" @default.
- W2034666807 title "Thermodynamic Analysis of the Binding of Aromatic Hydroxamic Acid Analogues to Ferric Horseradish Peroxidase" @default.
- W2034666807 cites W1531016194 @default.
- W2034666807 cites W1575373997 @default.
- W2034666807 cites W1589479912 @default.
- W2034666807 cites W1849424407 @default.
- W2034666807 cites W1969363109 @default.
- W2034666807 cites W1990052170 @default.
- W2034666807 cites W2019213007 @default.
- W2034666807 cites W2020372416 @default.
- W2034666807 cites W2024464505 @default.
- W2034666807 cites W2044672033 @default.
- W2034666807 cites W2094987170 @default.
- W2034666807 cites W2179092050 @default.
- W2034666807 cites W2418888804 @default.
- W2034666807 doi "https://doi.org/10.1021/bi010445f" @default.
- W2034666807 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11705389" @default.
- W2034666807 hasPublicationYear "2001" @default.
- W2034666807 type Work @default.
- W2034666807 sameAs 2034666807 @default.
- W2034666807 citedByCount "17" @default.
- W2034666807 countsByYear W20346668072015 @default.
- W2034666807 countsByYear W20346668072018 @default.
- W2034666807 countsByYear W20346668072019 @default.
- W2034666807 crossrefType "journal-article" @default.
- W2034666807 hasAuthorship W2034666807A5004492579 @default.
- W2034666807 hasAuthorship W2034666807A5057514326 @default.
- W2034666807 hasAuthorship W2034666807A5062598918 @default.
- W2034666807 hasAuthorship W2034666807A5064592336 @default.
- W2034666807 hasConcept C107824862 @default.
- W2034666807 hasConcept C121332964 @default.
- W2034666807 hasConcept C147789679 @default.
- W2034666807 hasConcept C156911925 @default.
- W2034666807 hasConcept C162008176 @default.
- W2034666807 hasConcept C178790620 @default.
- W2034666807 hasConcept C179104552 @default.
- W2034666807 hasConcept C181199279 @default.
- W2034666807 hasConcept C185592680 @default.
- W2034666807 hasConcept C2776217839 @default.
- W2034666807 hasConcept C2776281265 @default.
- W2034666807 hasConcept C2777676563 @default.
- W2034666807 hasConcept C2780534640 @default.
- W2034666807 hasConcept C3288061 @default.
- W2034666807 hasConcept C55493867 @default.
- W2034666807 hasConcept C71240020 @default.
- W2034666807 hasConcept C8010536 @default.
- W2034666807 hasConcept C97355855 @default.
- W2034666807 hasConceptScore W2034666807C107824862 @default.
- W2034666807 hasConceptScore W2034666807C121332964 @default.
- W2034666807 hasConceptScore W2034666807C147789679 @default.
- W2034666807 hasConceptScore W2034666807C156911925 @default.
- W2034666807 hasConceptScore W2034666807C162008176 @default.
- W2034666807 hasConceptScore W2034666807C178790620 @default.
- W2034666807 hasConceptScore W2034666807C179104552 @default.
- W2034666807 hasConceptScore W2034666807C181199279 @default.
- W2034666807 hasConceptScore W2034666807C185592680 @default.
- W2034666807 hasConceptScore W2034666807C2776217839 @default.
- W2034666807 hasConceptScore W2034666807C2776281265 @default.
- W2034666807 hasConceptScore W2034666807C2777676563 @default.
- W2034666807 hasConceptScore W2034666807C2780534640 @default.
- W2034666807 hasConceptScore W2034666807C3288061 @default.
- W2034666807 hasConceptScore W2034666807C55493867 @default.
- W2034666807 hasConceptScore W2034666807C71240020 @default.
- W2034666807 hasConceptScore W2034666807C8010536 @default.
- W2034666807 hasConceptScore W2034666807C97355855 @default.
- W2034666807 hasIssue "46" @default.
- W2034666807 hasLocation W20346668071 @default.
- W2034666807 hasLocation W20346668072 @default.
- W2034666807 hasOpenAccess W2034666807 @default.
- W2034666807 hasPrimaryLocation W20346668071 @default.
- W2034666807 hasRelatedWork W1980648788 @default.
- W2034666807 hasRelatedWork W1981974511 @default.
- W2034666807 hasRelatedWork W2002978594 @default.
- W2034666807 hasRelatedWork W2018809837 @default.
- W2034666807 hasRelatedWork W2028116489 @default.
- W2034666807 hasRelatedWork W2081358490 @default.
- W2034666807 hasRelatedWork W2090810684 @default.
- W2034666807 hasRelatedWork W2580576457 @default.
- W2034666807 hasRelatedWork W4205806953 @default.
- W2034666807 hasRelatedWork W4247752058 @default.
- W2034666807 hasVolume "40" @default.
- W2034666807 isParatext "false" @default.
- W2034666807 isRetracted "false" @default.
- W2034666807 magId "2034666807" @default.
- W2034666807 workType "article" @default.