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• W2034672438 startingPage "15" @default.
• W2034672438 abstract "The proteins PLM (phospholemman), CHIF (channel inducing factor), and Mat8 (mammary tumor protein 8 kDa) are members of the FXYD family of ion transport regulatory membrane proteins. Here we describe their cloning and expression in Escherichia coli, and their purification for NMR structural studies in lipid micelles and lipid bilayers. The molecular masses of the purified recombinant FXYD proteins, determined from SDS-PAGE and from MALDI TOF mass spectrometry, reflect monomeric species. The solution NMR and CD spectra in SDS micelles show that they adopt helical conformations. The solid-state NMR spectra in lipid bilayers give the first view of their transmembrane architecture." @default.
• W2034672438 created "2016-06-24" @default.
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• W2034672438 date "2003-01-01" @default.
• W2034672438 modified "2023-10-13" @default.
• W2034672438 title "Expression and characterization of the FXYD ion transport regulators for NMR structural studies in lipid micelles and lipid bilayers" @default.
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• W2034672438 doi "https://doi.org/10.1016/s1570-9639(02)00473-9" @default.
• W2034672438 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2917601" @default.
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• W2034672438 hasPublicationYear "2003" @default.
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