Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034673280> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2034673280 endingPage "59" @default.
- W2034673280 startingPage "54" @default.
- W2034673280 abstract "Developing assays for detecting HCV antigens could be beneficial because viral proteins appear earlier than antibodies and are more stable than RNA in the serum. Monoclonal antibody was prepared by immunization and cell fusion. Subclass, specificity, and efficiency of monoclonal antibodies were determined by ELISA. Epitope specificity of monoclonal antibodies was analyzed by ELISA additivity test. HCV antigen in serum of hepatitis patients was examined by double monoclonal antibody sandwich ELISA. Five hybridoma cell lines were screened and named HCV1, HCV2, HCV3, HCV4, and HCV5. These five monoclonal antibodies had high specificity and efficiency. The additivity test showed that HCV2, HCV4, and HCV5 recognized different epitopes, which can be matched in ELISA. Of 173 anti-HCV positive patients, 37 (21.4%) were positive for HCV antigen. Of 1498 anti-HCV negative patients, 10 (0.67%) were positive for HCV antigen. Fifty normal controls were negative for HCV antigen. HCV antigen detection had moderate agreement and correlation with HCV RNA detection (kappa=0.577, p<0.01; r=0.59, p<0.01). This result indicates that the monoclonal antibody against HCV NS3 may be a potential diagnostic reagent, which would provide a foundation for developing a sandwich ELISA of HCV antigen detection." @default.
- W2034673280 created "2016-06-24" @default.
- W2034673280 creator A5028162489 @default.
- W2034673280 creator A5030159829 @default.
- W2034673280 creator A5053309160 @default.
- W2034673280 creator A5068675597 @default.
- W2034673280 creator A5078825725 @default.
- W2034673280 date "2012-02-01" @default.
- W2034673280 modified "2023-10-16" @default.
- W2034673280 title "Characterization and Application of Monoclonal Antibody Against Hepatitis C Virus Nonstructual Protein Three" @default.
- W2034673280 cites W1908985082 @default.
- W2034673280 cites W1991926991 @default.
- W2034673280 cites W1998093640 @default.
- W2034673280 cites W2006309458 @default.
- W2034673280 cites W2020004919 @default.
- W2034673280 cites W2026533131 @default.
- W2034673280 cites W2040108618 @default.
- W2034673280 cites W2052273800 @default.
- W2034673280 cites W2052519040 @default.
- W2034673280 cites W2059749879 @default.
- W2034673280 cites W2060664918 @default.
- W2034673280 cites W2062202843 @default.
- W2034673280 cites W2071911038 @default.
- W2034673280 cites W2078849853 @default.
- W2034673280 cites W2081031967 @default.
- W2034673280 cites W2083427399 @default.
- W2034673280 cites W2098343844 @default.
- W2034673280 cites W2101084503 @default.
- W2034673280 cites W2135791975 @default.
- W2034673280 cites W2153923196 @default.
- W2034673280 cites W2158974921 @default.
- W2034673280 cites W2159540680 @default.
- W2034673280 cites W2167312953 @default.
- W2034673280 cites W2403078466 @default.
- W2034673280 cites W3030748067 @default.
- W2034673280 doi "https://doi.org/10.1089/hyb.2011.0076" @default.
- W2034673280 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22316486" @default.
- W2034673280 hasPublicationYear "2012" @default.
- W2034673280 type Work @default.
- W2034673280 sameAs 2034673280 @default.
- W2034673280 citedByCount "0" @default.
- W2034673280 crossrefType "journal-article" @default.
- W2034673280 hasAuthorship W2034673280A5028162489 @default.
- W2034673280 hasAuthorship W2034673280A5030159829 @default.
- W2034673280 hasAuthorship W2034673280A5053309160 @default.
- W2034673280 hasAuthorship W2034673280A5068675597 @default.
- W2034673280 hasAuthorship W2034673280A5078825725 @default.
- W2034673280 hasConcept C132379496 @default.
- W2034673280 hasConcept C147483822 @default.
- W2034673280 hasConcept C153911025 @default.
- W2034673280 hasConcept C159047783 @default.
- W2034673280 hasConcept C159654299 @default.
- W2034673280 hasConcept C163864487 @default.
- W2034673280 hasConcept C195616568 @default.
- W2034673280 hasConcept C203014093 @default.
- W2034673280 hasConcept C2522874641 @default.
- W2034673280 hasConcept C2776194381 @default.
- W2034673280 hasConcept C2776408679 @default.
- W2034673280 hasConcept C542903549 @default.
- W2034673280 hasConcept C86803240 @default.
- W2034673280 hasConceptScore W2034673280C132379496 @default.
- W2034673280 hasConceptScore W2034673280C147483822 @default.
- W2034673280 hasConceptScore W2034673280C153911025 @default.
- W2034673280 hasConceptScore W2034673280C159047783 @default.
- W2034673280 hasConceptScore W2034673280C159654299 @default.
- W2034673280 hasConceptScore W2034673280C163864487 @default.
- W2034673280 hasConceptScore W2034673280C195616568 @default.
- W2034673280 hasConceptScore W2034673280C203014093 @default.
- W2034673280 hasConceptScore W2034673280C2522874641 @default.
- W2034673280 hasConceptScore W2034673280C2776194381 @default.
- W2034673280 hasConceptScore W2034673280C2776408679 @default.
- W2034673280 hasConceptScore W2034673280C542903549 @default.
- W2034673280 hasConceptScore W2034673280C86803240 @default.
- W2034673280 hasIssue "1" @default.
- W2034673280 hasLocation W20346732801 @default.
- W2034673280 hasLocation W20346732802 @default.
- W2034673280 hasOpenAccess W2034673280 @default.
- W2034673280 hasPrimaryLocation W20346732801 @default.
- W2034673280 hasRelatedWork W1679554208 @default.
- W2034673280 hasRelatedWork W1973692620 @default.
- W2034673280 hasRelatedWork W1986429294 @default.
- W2034673280 hasRelatedWork W2031966787 @default.
- W2034673280 hasRelatedWork W2034673280 @default.
- W2034673280 hasRelatedWork W2060284918 @default.
- W2034673280 hasRelatedWork W2070226647 @default.
- W2034673280 hasRelatedWork W2078659469 @default.
- W2034673280 hasRelatedWork W2415962821 @default.
- W2034673280 hasRelatedWork W2461002291 @default.
- W2034673280 hasVolume "31" @default.
- W2034673280 isParatext "false" @default.
- W2034673280 isRetracted "false" @default.
- W2034673280 magId "2034673280" @default.
- W2034673280 workType "article" @default.