FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034677367> ?p ?o ?g. }

• W2034677367 endingPage "5185" @default.
• W2034677367 startingPage "5176" @default.
• W2034677367 abstract "The heterodimeric nuclear factor (NF) 90/NF45 complex (NF90/NF45) binds nucleic acids and is a multifunctional regulator of gene expression. Here we report that depletion of NF90/NF45 restores the expression of the p53 and p21 proteins in cervical carcinoma cells infected with high-risk human papillomaviruses (HPVs). Knockdown of either NF90 or NF45 by RNA interference led to greatly elevated levels of p53 and p21 proteins in HPV-derived HeLa and SiHa cells but not in other cancerous or normal cell lines. In HeLa cells, p21 messenger-RNA (mRNA) increased concomitantly but the level of p53 mRNA was unaffected. RNA interference directed against p53 prevented the induction of both proteins. These results indicated that the upregulation of p21 is due to p53-dependent transcription, whereas p53 is regulated post-transcriptionally. Proteasome-mediated turnover of p53 is accelerated by the HPV E6 and cellular E6AP proteins. We therefore examined the hypothesis that this pathway is regulated by NF90/NF45. Indeed, depletion of NF90 attenuated the expression of E6 RNA and inhibited transcription from the HPV early promoter, revealing a new role for NF90/NF45 in HPV gene expression. The transcription inhibition was largely independent of the reduction of P-TEFb (positive transcription elongation factor b) levels caused by NF90 depletion. Consistent with p53 derepression, NF90/NF45-depleted HeLa cells displayed elevated poly ADP-ribose polymerase (PARP) cleavage and susceptibility to camptothecin-induced apoptosis. We conclude that high-risk strains of HPV utilize the cellular NF90/NF45 complex for viral E6 expression in infected cervical carcinoma cell lines. Interference with NF90/NF45 function could assist in controlling cervical carcinoma." @default.
• W2034677367 created "2016-06-24" @default.
• W2034677367 creator A5018004018 @default.
• W2034677367 creator A5022089118 @default.
• W2034677367 creator A5024099273 @default.
• W2034677367 creator A5091074558 @default.
• W2034677367 date "2012-12-03" @default.
• W2034677367 modified "2023-09-30" @default.
• W2034677367 title "Induction of p53, p21 and apoptosis by silencing the NF90/NF45 complex in human papilloma virus-transformed cervical carcinoma cells" @default.
• W2034677367 cites W140061535 @default.
• W2034677367 cites W1557386862 @default.
• W2034677367 cites W1562917910 @default.
• W2034677367 cites W1595083214 @default.
• W2034677367 cites W1650797637 @default.
• W2034677367 cites W1912001846 @default.
• W2034677367 cites W1965047713 @default.
• W2034677367 cites W1965086052 @default.
• W2034677367 cites W1967590733 @default.
• W2034677367 cites W1967925876 @default.
• W2034677367 cites W1968862380 @default.
• W2034677367 cites W1970198307 @default.
• W2034677367 cites W1972654719 @default.
• W2034677367 cites W1973975093 @default.
• W2034677367 cites W1973975456 @default.
• W2034677367 cites W1975922979 @default.
• W2034677367 cites W1982949581 @default.
• W2034677367 cites W1983883289 @default.
• W2034677367 cites W1985102026 @default.
• W2034677367 cites W1989439523 @default.
• W2034677367 cites W1990433648 @default.
• W2034677367 cites W1999787047 @default.
• W2034677367 cites W2003744982 @default.
• W2034677367 cites W2007614033 @default.
• W2034677367 cites W2007699219 @default.
• W2034677367 cites W2011331611 @default.
• W2034677367 cites W2011363727 @default.
• W2034677367 cites W2011499737 @default.
• W2034677367 cites W2018385269 @default.
• W2034677367 cites W2020329201 @default.
• W2034677367 cites W2027243820 @default.
• W2034677367 cites W2028791725 @default.
• W2034677367 cites W2029194165 @default.
• W2034677367 cites W2030922603 @default.
• W2034677367 cites W2034182181 @default.
• W2034677367 cites W2038173255 @default.
• W2034677367 cites W2039382131 @default.
• W2034677367 cites W2040633917 @default.
• W2034677367 cites W2041807155 @default.
• W2034677367 cites W2042695180 @default.
• W2034677367 cites W2045402423 @default.
• W2034677367 cites W2047705692 @default.
• W2034677367 cites W2052651806 @default.
• W2034677367 cites W2053416557 @default.
• W2034677367 cites W2053847776 @default.
• W2034677367 cites W2058217451 @default.
• W2034677367 cites W2066030187 @default.
• W2034677367 cites W2072088194 @default.
• W2034677367 cites W2078532650 @default.
• W2034677367 cites W2078581194 @default.
• W2034677367 cites W2080018502 @default.
• W2034677367 cites W2081056759 @default.
• W2034677367 cites W2081894694 @default.
• W2034677367 cites W2084076352 @default.
• W2034677367 cites W2086782356 @default.
• W2034677367 cites W2092537625 @default.
• W2034677367 cites W2092913179 @default.
• W2034677367 cites W2093710784 @default.
• W2034677367 cites W2094304571 @default.
• W2034677367 cites W2096371557 @default.
• W2034677367 cites W2096521070 @default.
• W2034677367 cites W2097623387 @default.
• W2034677367 cites W2098215046 @default.
• W2034677367 cites W2098791327 @default.
• W2034677367 cites W2100539809 @default.
• W2034677367 cites W2102421724 @default.
• W2034677367 cites W2103237568 @default.
• W2034677367 cites W2109545571 @default.
• W2034677367 cites W2109751513 @default.
• W2034677367 cites W2112682271 @default.
• W2034677367 cites W2114172480 @default.
• W2034677367 cites W2114262960 @default.
• W2034677367 cites W2115856463 @default.
• W2034677367 cites W2120328543 @default.
• W2034677367 cites W2122192041 @default.
• W2034677367 cites W2128337337 @default.
• W2034677367 cites W2129081421 @default.
• W2034677367 cites W2129424750 @default.
• W2034677367 cites W2130946797 @default.
• W2034677367 cites W2133896397 @default.
• W2034677367 cites W2134386213 @default.
• W2034677367 cites W2143677057 @default.
• W2034677367 cites W2146606416 @default.
• W2034677367 cites W2147339550 @default.
• W2034677367 cites W2147373017 @default.
• W2034677367 cites W2148186824 @default.
• W2034677367 cites W2148330261 @default.
• W2034677367 cites W2150049406 @default.
• W2034677367 cites W2150525034 @default.

• next