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• W2034679908 abstract "Hepatitis B virus (HBV) variants with a nonsense mutation in the distal pre-C region have been detected in patients positive for anti-H Be, and the complete nucleotide sequence of one cloned pre-C variant has been determined. Transfection of this HBV variant clone into the human hepatoma cell line HepG2 resulted in the appearance of major HBV transcripts, replicative forms of viral DNA evidenced by both molecular hybridization and endogenous DNA polymerase assay, as well as the expression and secretion of HBcAg and HBcAg particles. Western blotting revealed only the 21-kDa HBcAg but not the 17-kDa HBeAg. These results demonstrate the replication capacity of the HBV variant with a nonfunctional pre-C region despite its inability to express HBeAg." @default.
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• W2034679908 date "1991-04-01" @default.
• W2034679908 modified "2023-09-23" @default.
• W2034679908 title "In vitro replication competence of a cloned hepatitis B virus variant with a nonsense mutation in the distal pre-C region" @default.
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• W2034679908 doi "https://doi.org/10.1016/0042-6822(91)90908-t" @default.
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