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- W2034683558 abstract "2-Thiouracil treatment leads to a marked suppression of incorporation of 32P into duplex viral RNA in TMV-infected tobacco leaves and TYMV-infected chinese cabbage leaves. The same treatment has little effect on the incorporation of 32P into host RNA. 35S-labelled 2-thiouracil is incorporated into the normal RNA of both tobacco and chinese cabbage leaves and into TMV viral duplex RNA. Under similar conditions no incorporation could be detected into TYMV duplex RNA. In leaves infected with TYMV, 2-thiouracil treatment did not lead to the disappearance of duplex viral RNA present at the time treatments were begun. TYMV infection, or 2-thiouracil treatment of either healthy or TYMV-infected leaves had no marked effect on the proportion of leaf ribosomes in polyribosome form. Empty TYMV protein shells produced during 2-thiouracil treatment were found to have an altered ultraviolet absorption spectrum and changed stability to heating. We suggest that 2-thiouracil has two major effects on TYMV synthesis: (i) it prevents RNA synthesis on the duplex RNA, leading to a marked reduction in viral RNA production, (ii) it leads to the production of abnormal viral protein shells through interference with the coding mechanism either by being incorporated into a small amount of viral RNA used for message or into host transfer RNA or both." @default.
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- W2034683558 date "1965-09-01" @default.
- W2034683558 modified "2023-09-27" @default.
- W2034683558 title "Effects of 2-thiouracil on the formation of double-stranded plant viral ribonucleic acid" @default.
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- W2034683558 doi "https://doi.org/10.1016/0005-2787(65)90107-3" @default.
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