FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034687407> ?p ?o ?g. }

• W2034687407 endingPage "103" @default.
• W2034687407 startingPage "95" @default.
• W2034687407 abstract "The fetal lung secretes significant quantities of surfactant during late gestation to prepare for initiation of respiration at birth. However, the mechanism by which this occurs has not been determined. Since Ca2+-phosphatidylserine (PS)-dependent protein kinase C has been implicated in surfactant secretion in adult lung, the present study was done to determine whether this enzyme is also involved in the initiation of surfactant release from fetal type II cells. Type II cells isolated from gestational day-24 fetal rabbits were used. Cells were prelabelled with [32P] and [3H]choline and exposed to 4beta phorbol ester (10(-5) M) for 2 h. Secretion product and subcellular fractions were isolated by removing the culture medium, mixing with homogenate from adult rabbit lung, and subfractionating by centrifugation on a sucrose gradient. Samples of secretion product were also prepared for electron microscopy. Ca2+-PS-dependent protein kinase C was also assayed in some samples, and an add-back technique was used to determine whether enzyme activity in the intracellularly stored surfactant fraction was due to contamination. The results showed that material released by fetal type II cells after exposure to phorbol ester coprecipitated with adult rabbit lung lamellar bodies and microsomes. Morphologically, a range of forms, including lamellar-body-like structures, was detected. The released material originated largely from the lamellar body compartment of the fetal type II cells and displayed immunoreactivity with antibody to surfactant protein A (SP-A) at 35 and 70 kDa apparent molecular mass. Assay of protein kinase C in fetal type II cells showed that exposure to conditioned medium, which induces differentiation, increased activity. Incubation with phorbol ester induced translocation of activity to the microsomal fraction. Add-back assays suggested that protein kinase C activation by treatment with phorbol ester induced translocation of enzyme activity to the lamellar body fraction; none was detected prior to treatment. These results support a role for Ca2+-PS-dependent protein kinase C in initiation of surfactant release by interaction with the developing lamellar body compartment in fetal type II cells." @default.
• W2034687407 created "2016-06-24" @default.
• W2034687407 creator A5020240333 @default.
• W2034687407 creator A5041091125 @default.
• W2034687407 creator A5052229367 @default.
• W2034687407 creator A5053323240 @default.
• W2034687407 creator A5067912247 @default.
• W2034687407 creator A5069706859 @default.
• W2034687407 creator A5072549526 @default.
• W2034687407 date "1999-02-01" @default.
• W2034687407 modified "2023-09-27" @default.
• W2034687407 title "Calcium-PS-dependent protein kinase C and surfactant protein A in isolated fetal rabbit type II alveolar cells and surfactant-related material" @default.
• W2034687407 cites W148785554 @default.
• W2034687407 cites W1588018069 @default.
• W2034687407 cites W1650137855 @default.
• W2034687407 cites W1889564238 @default.
• W2034687407 cites W1973870362 @default.
• W2034687407 cites W1974935223 @default.
• W2034687407 cites W1976662895 @default.
• W2034687407 cites W1977013639 @default.
• W2034687407 cites W1978340202 @default.
• W2034687407 cites W1990623709 @default.
• W2034687407 cites W1992282523 @default.
• W2034687407 cites W1996389622 @default.
• W2034687407 cites W2003065842 @default.
• W2034687407 cites W2004176673 @default.
• W2034687407 cites W2015571904 @default.
• W2034687407 cites W2017069757 @default.
• W2034687407 cites W2018173590 @default.
• W2034687407 cites W2052588188 @default.
• W2034687407 cites W2054682952 @default.
• W2034687407 cites W2056539191 @default.
• W2034687407 cites W2066789314 @default.
• W2034687407 cites W2129224957 @default.
• W2034687407 cites W2129882506 @default.
• W2034687407 cites W2164336695 @default.
• W2034687407 cites W2170372880 @default.
• W2034687407 cites W2172048985 @default.
• W2034687407 cites W2174520807 @default.
• W2034687407 cites W2234034921 @default.
• W2034687407 cites W2287094018 @default.
• W2034687407 cites W2289744294 @default.
• W2034687407 cites W2306890945 @default.
• W2034687407 cites W2409164624 @default.
• W2034687407 cites W52918786 @default.
• W2034687407 doi "https://doi.org/10.1002/(sici)1099-0496(199902)27:2<95::aid-ppul5>3.0.co;2-5" @default.
• W2034687407 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10088932" @default.
• W2034687407 hasPublicationYear "1999" @default.
• W2034687407 type Work @default.
• W2034687407 sameAs 2034687407 @default.
• W2034687407 citedByCount "6" @default.
• W2034687407 crossrefType "journal-article" @default.
• W2034687407 hasAuthorship W2034687407A5020240333 @default.
• W2034687407 hasAuthorship W2034687407A5041091125 @default.
• W2034687407 hasAuthorship W2034687407A5052229367 @default.
• W2034687407 hasAuthorship W2034687407A5053323240 @default.
• W2034687407 hasAuthorship W2034687407A5067912247 @default.
• W2034687407 hasAuthorship W2034687407A5069706859 @default.
• W2034687407 hasAuthorship W2034687407A5072549526 @default.
• W2034687407 hasConcept C145156650 @default.
• W2034687407 hasConcept C153911025 @default.
• W2034687407 hasConcept C172680121 @default.
• W2034687407 hasConcept C184235292 @default.
• W2034687407 hasConcept C195794163 @default.
• W2034687407 hasConcept C2779234561 @default.
• W2034687407 hasConcept C49039625 @default.
• W2034687407 hasConcept C54355233 @default.
• W2034687407 hasConcept C55493867 @default.
• W2034687407 hasConcept C58226133 @default.
• W2034687407 hasConcept C86803240 @default.
• W2034687407 hasConceptScore W2034687407C145156650 @default.
• W2034687407 hasConceptScore W2034687407C153911025 @default.
• W2034687407 hasConceptScore W2034687407C172680121 @default.
• W2034687407 hasConceptScore W2034687407C184235292 @default.
• W2034687407 hasConceptScore W2034687407C195794163 @default.
• W2034687407 hasConceptScore W2034687407C2779234561 @default.
• W2034687407 hasConceptScore W2034687407C49039625 @default.
• W2034687407 hasConceptScore W2034687407C54355233 @default.
• W2034687407 hasConceptScore W2034687407C55493867 @default.
• W2034687407 hasConceptScore W2034687407C58226133 @default.
• W2034687407 hasConceptScore W2034687407C86803240 @default.
• W2034687407 hasIssue "2" @default.
• W2034687407 hasLocation W20346874071 @default.
• W2034687407 hasLocation W20346874072 @default.
• W2034687407 hasOpenAccess W2034687407 @default.
• W2034687407 hasPrimaryLocation W20346874071 @default.
• W2034687407 hasRelatedWork W1513592346 @default.
• W2034687407 hasRelatedWork W1999667493 @default.
• W2034687407 hasRelatedWork W2042903540 @default.
• W2034687407 hasRelatedWork W2048107595 @default.
• W2034687407 hasRelatedWork W2049777592 @default.
• W2034687407 hasRelatedWork W2111232427 @default.
• W2034687407 hasRelatedWork W2113455304 @default.
• W2034687407 hasRelatedWork W2402425702 @default.
• W2034687407 hasRelatedWork W2403238256 @default.
• W2034687407 hasRelatedWork W2608763396 @default.
• W2034687407 hasVolume "27" @default.
• W2034687407 isParatext "false" @default.

• next