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- W2034690879 abstract "Recombinant human mu-calpain whose active site Cys-115 was substituted with Ser was expressed in insect cells using baculovirus system. The mutant mu-calpain, purified using an affinity-column of calpastatin oligopeptides, had no proteolytic activities of autolysis and caseinolysis. The large subunit of the mutant mu-calpain was processed from the 80 kDa form to the 76 kDa form by the wild type calpain, supporting the intermolecular cleavage mechanism of procalpain during activation. Fluorescence polarization analysis revealed that the mutant mu-calpain retained high affinity toward fluorescein-labeled calpastatin domain 1. Fragmentation of the full-length calpastatin by the wild type calpain was enhanced by pre-incubating the inhibitor with the mutant calpain. The recombinant mutant calpain was suggested to retain the integrity of the high ordered structure of the wild type calpain." @default.
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- W2034690879 date "1998-05-01" @default.
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- W2034690879 title "Purification and Characterization of the Active-Site-Mutated Recombinant Human μ-Calpain Expressed in Baculovirus-Infected Insect Cells" @default.
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- W2034690879 doi "https://doi.org/10.1006/bbrc.1998.8686" @default.
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