FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034691945> ?p ?o ?g. }

• W2034691945 endingPage "1682" @default.
• W2034691945 startingPage "1673" @default.
• W2034691945 abstract "The hyperacute rejection caused by preformed natural antibodies in the recipient species reacting with donor species endothelial antigens is one of the major obstacles preventing routine use of clinical xenotransplantation. Based on the known structure and biosynthetic pathway of the major porcine xenoantigen, Gal alpha1,3Gal, we developed a novel strategy aimed at specific removal of human, natural anti-pig antibodies.Cotransfection of COS cells with expression plasmids encoding a secreted mucin/immunoglobulin chimera and the porcine alpha1,3-galactosyltransferase facilitated simple immunoaffinity purification of a highly Gal alpha1,3Gal-substituted mucin/immunoglobulin fusion protein from transfected cell supernatants.Cotransfection of COS cells resulted in a mucin/Ig concentration in the supernatants ranging from 150 to 200 ng/ml. Approximately 300 ng of mucin/Ig chimeras absorbed onto 50 microl of packed anti-mouse IgG agarose beads could completely remove cytotoxic human anti-pig antibodies from 1 ml of human AB serum, as estimated in porcine endothelial cell cytotoxicity assays. Purified human IgG, IgM, and IgA all bound porcine endothelium, but only IgG and IgM were cytotoxic in the presence of rabbit complement. When the cytotoxicity of human IgG at 8 mg/ml and IgM at 1 mg/ml was completely removed by absorption on the mucin/Ig chimera, the binding to porcine endothelium was only partly reduced. Antibodies mediating antibody-dependent cellular cytotoxicity against porcine endothelium were also absorbed, indicating the importance of Gal alpha1,3Gal epitopes for this effect.We describe the construction and production of a new and effective Gal alpha1,3Gal-substituted, mucin domain-containing absorber that can be used in a pretransplant extracorporeal immunoabsorption setting to remove anti-pig antibodies involved in antibody-dependent, complement- and cell-mediated cytotoxicity of pig endothelial cells." @default.
• W2034691945 created "2016-06-24" @default.
• W2034691945 creator A5001911474 @default.
• W2034691945 creator A5047063961 @default.
• W2034691945 creator A5067351861 @default.
• W2034691945 date "1997-06-01" @default.
• W2034691945 modified "2023-10-02" @default.
• W2034691945 title "REMOVAL OF XENOREACTIVE HUMAN ANTI-PIG ANTIBODIES BY ABSORPTION ON RECOMBINANT MUCIN-CONTAINING GLYCOPROTEINS CARRYING THE GAL??1,3GAL EPITOPE1" @default.
• W2034691945 cites W1512073660 @default.
• W2034691945 cites W1966469614 @default.
• W2034691945 cites W1970966609 @default.
• W2034691945 cites W1973173142 @default.
• W2034691945 cites W1974299002 @default.
• W2034691945 cites W1976954156 @default.
• W2034691945 cites W1978424754 @default.
• W2034691945 cites W1980726194 @default.
• W2034691945 cites W1981019503 @default.
• W2034691945 cites W1988632462 @default.
• W2034691945 cites W1990370275 @default.
• W2034691945 cites W2001612894 @default.
• W2034691945 cites W2003121008 @default.
• W2034691945 cites W2009722410 @default.
• W2034691945 cites W2013640301 @default.
• W2034691945 cites W2018204952 @default.
• W2034691945 cites W2020321980 @default.
• W2034691945 cites W2020909382 @default.
• W2034691945 cites W2033430917 @default.
• W2034691945 cites W2037198722 @default.
• W2034691945 cites W2038314259 @default.
• W2034691945 cites W2041963881 @default.
• W2034691945 cites W2044006164 @default.
• W2034691945 cites W2045235394 @default.
• W2034691945 cites W2046975694 @default.
• W2034691945 cites W2047916715 @default.
• W2034691945 cites W2048769067 @default.
• W2034691945 cites W2052847242 @default.
• W2034691945 cites W2054736088 @default.
• W2034691945 cites W2059171126 @default.
• W2034691945 cites W2059645048 @default.
• W2034691945 cites W2067599992 @default.
• W2034691945 cites W2072738331 @default.
• W2034691945 cites W2075350569 @default.
• W2034691945 cites W2079304688 @default.
• W2034691945 cites W2080224134 @default.
• W2034691945 cites W2080932583 @default.
• W2034691945 cites W2083502535 @default.
• W2034691945 cites W2084279863 @default.
• W2034691945 cites W2085403237 @default.
• W2034691945 cites W2086870938 @default.
• W2034691945 cites W2090226527 @default.
• W2034691945 cites W2100837269 @default.
• W2034691945 cites W2101108802 @default.
• W2034691945 cites W2103391840 @default.
• W2034691945 cites W2121996637 @default.
• W2034691945 cites W2155507510 @default.
• W2034691945 cites W2167012006 @default.
• W2034691945 cites W2347063821 @default.
• W2034691945 cites W240431119 @default.
• W2034691945 cites W4231035875 @default.
• W2034691945 cites W2030072395 @default.
• W2034691945 doi "https://doi.org/10.1097/00007890-199706150-00023" @default.
• W2034691945 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9197365" @default.
• W2034691945 hasPublicationYear "1997" @default.
• W2034691945 type Work @default.
• W2034691945 sameAs 2034691945 @default.
• W2034691945 citedByCount "42" @default.
• W2034691945 countsByYear W20346919452012 @default.
• W2034691945 countsByYear W20346919452013 @default.
• W2034691945 countsByYear W20346919452015 @default.
• W2034691945 countsByYear W20346919452018 @default.
• W2034691945 countsByYear W20346919452020 @default.
• W2034691945 countsByYear W20346919452022 @default.
• W2034691945 crossrefType "journal-article" @default.
• W2034691945 hasAuthorship W2034691945A5001911474 @default.
• W2034691945 hasAuthorship W2034691945A5047063961 @default.
• W2034691945 hasAuthorship W2034691945A5067351861 @default.
• W2034691945 hasBestOaLocation W20346919451 @default.
• W2034691945 hasConcept C108625454 @default.
• W2034691945 hasConcept C109316439 @default.
• W2034691945 hasConcept C141071460 @default.
• W2034691945 hasConcept C147483822 @default.
• W2034691945 hasConcept C153911025 @default.
• W2034691945 hasConcept C154317977 @default.
• W2034691945 hasConcept C159654299 @default.
• W2034691945 hasConcept C179264091 @default.
• W2034691945 hasConcept C185592680 @default.
• W2034691945 hasConcept C195616568 @default.
• W2034691945 hasConcept C202751555 @default.
• W2034691945 hasConcept C203014093 @default.
• W2034691945 hasConcept C2778442404 @default.
• W2034691945 hasConcept C2911091166 @default.
• W2034691945 hasConcept C55493867 @default.
• W2034691945 hasConcept C71924100 @default.
• W2034691945 hasConcept C86803240 @default.
• W2034691945 hasConceptScore W2034691945C108625454 @default.
• W2034691945 hasConceptScore W2034691945C109316439 @default.
• W2034691945 hasConceptScore W2034691945C141071460 @default.
• W2034691945 hasConceptScore W2034691945C147483822 @default.

• next